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CDS information : A4092_00180


close this sectionLocation

Organism
StrainATCC 39727
Entry nameA40926
Contig
Start / Stop / Direction22,221 / 21,025 / - [in whole cluster]
22,221 / 21,025 / - [in contig]
Locationcomplement(21025..22221) [in whole cluster]
complement(21025..22221) [in contig]
TypeCDS
Length1,197 bp (398 aa)
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close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)putative OxyB monooxigenase
Genedbv12
oxyB
Gene (GenBank)dbv12
EC number
Keyword
  • 1st cross-linking
  • C-O-D ring
Note
Note (GenBank)
Reference
ACC
PmId
[12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003)
[17873036] Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. (J Bacteriol. , 2007)
[25986904] Two Master Switch Regulators Trigger A40926 Biosynthesis in Nonomuraea sp. Strain ATCC 39727. (J Bacteriol. , 2015)
Related Reference
ACC
O87674
NITE
Balhi_00120
PmId
[10390204] Identification and analysis of the balhimycin biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908. (Antimicrob Agents Chemother. , 1999)
[12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001)
[15651041] The biosynthesis of vancomycin-type glycopeptide antibiotics--a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. (Chembiochem. , 2005)
[16730832] Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. (J Biotechnol. , 2006)
[20337711] Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis. (FEMS Microbiol Lett. , 2010)
ACC
Q8RN04
PmId
[12207020] Crystal structure of OxyB, a cytochrome P450 implicated in an oxidative phenol coupling reaction during vancomycin biosynthesis. (J Biol Chem. , 2002)
[15593150] An oxidative phenol coupling reaction catalyzed by oxyB, a cytochrome P450 from the vancomycin-producing microorganism. (Angew Chem Int Ed Engl. , 2004)
[17477533] Oxidative phenol coupling reactions catalyzed by OxyB: a cytochrome P450 from the vancomycin producing organism. implications for vancomycin biosynthesis. (J Am Chem Soc. , 2007)
[18068978] New insights into the first oxidative phenol coupling reaction during vancomycin biosynthesis. (Bioorg Med Chem Lett. , 2008)
[18688478] Exploring the substrate specificity of OxyB, a phenol coupling P450 enzyme involved in vancomycin biosynthesis. (Org Biomol Chem. , 2008)
[20337711] Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis. (FEMS Microbiol Lett. , 2010)
[23073849] Substituent effects on the phenol coupling reaction catalyzed by the vancomycin biosynthetic P450 enzyme OxyB. (Angew Chem Int Ed Engl. , 2012)
ACC
Q8KLL9
NITE
A4793_00070
PmId
[17884639] The biosynthesis of teicoplanin-type glycopeptide antibiotics: assignment of p450 mono-oxygenases to side chain cyclizations of glycopeptide a47934. (Chem Biol. , 2007)
ACC
Q70AY8
NITE
Teicp2_00290
PmId
[25686610] X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. (Nature. , 2015)
[26549530] Sequential In Vitro Cyclization by Cytochrome P450 Enzymes of Glycopeptide Antibiotic Precursors Bearing the X-Domain from Nonribosomal Peptide Biosynthesis. (Angew Chem Int Ed Engl. , 2015)
[27213615] Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. (J Am Chem Soc. , 2016)
ACC
Q6ZZI6
NITE
Teicp_00220
PmId
[25358800] Cytochrome P450 OxyBtei catalyzes the first phenolic coupling step in teicoplanin biosynthesis. (Chembiochem. , 2014)

close this sectionSequence

selected fasta
>putative cytochrome P450 [putative OxyB monooxigenase]
MSGDGARPLHTRRQDLDPADELRAAGTLTRITIGSGADAETTWLATGYTVVRQVLGDHRR
FSTRRRWNERDEIGGRGNFRPRELVGNLMDYDPPEHTRLRQKLTPGFTLRRIRRLKPYIE
QIVTERLDALERAGPPADLVELVADEVPGAVLCELIGVPRDDRAMFMQLCHGHLDASRSQ
KRRAAAGAAFSRYLLAMIARERKDPGEGLLGAVLAEYGDTATDEELRGFCVQVMLAGDDN
ISGMIGLGVLALLRHPEQIAALQGDDQSADRAVDELIRYLTVPYAPTPRVAMEDVTIGGQ
VIKEGETVSCSLPMANRDPALLPDAGRLDVRREPVPHVAFGHGVHHCLGAALARLELRTV
YTALWRRFPTLRLADPDREPSFRLTTPAYGLTSLMVAW
selected fasta
>putative cytochrome P450 [putative OxyB monooxigenase]
TTGAGTGGTGACGGCGCGCGGCCCCTCCATACTCGGCGGCAGGACCTGGATCCGGCGGAC
GAACTGCGCGCCGCCGGAACGCTGACGAGGATCACCATCGGATCAGGAGCGGACGCGGAG
ACCACCTGGCTGGCCACGGGGTACACCGTCGTGCGGCAGGTGCTCGGCGATCACCGGCGG
TTCAGCACCCGGCGCCGCTGGAACGAACGGGACGAGATCGGCGGAAGAGGCAACTTCCGG
CCGCGTGAGCTGGTCGGGAACCTGATGGACTACGACCCGCCTGAGCACACGCGGCTCCGC
CAGAAGCTGACCCCGGGATTCACCCTGCGCCGGATCCGGCGGCTGAAGCCGTACATCGAA
CAGATCGTGACCGAACGGCTCGACGCCCTGGAACGGGCGGGCCCCCCTGCGGATCTGGTC
GAACTCGTCGCCGACGAGGTGCCAGGGGCCGTGCTGTGCGAGCTGATCGGGGTGCCACGG
GACGACCGCGCCATGTTCATGCAGCTGTGTCACGGCCATCTCGACGCCTCGCGCAGCCAG
AAGAGGCGGGCGGCCGCCGGCGCGGCGTTCTCCCGCTACCTGCTGGCCATGATCGCCAGG
GAGCGGAAGGATCCCGGCGAGGGGCTGCTCGGAGCCGTCCTCGCCGAGTACGGTGACACG
GCCACGGACGAGGAGCTGCGGGGCTTCTGCGTTCAGGTGATGCTGGCCGGCGACGACAAC
ATCTCCGGCATGATCGGGCTCGGCGTGCTGGCCCTGCTCAGACACCCCGAGCAGATCGCC
GCGTTGCAGGGCGACGACCAGTCGGCGGATCGGGCGGTCGACGAGCTGATCCGCTACCTG
ACCGTCCCCTATGCGCCGACGCCCCGGGTCGCCATGGAGGACGTCACCATCGGGGGCCAG
GTGATCAAGGAGGGAGAGACCGTCTCCTGCTCTCTGCCCATGGCCAACCGTGACCCTGCC
CTGCTGCCGGATGCCGGCCGCCTCGATGTCCGGCGCGAGCCCGTCCCCCATGTCGCGTTC
GGGCACGGCGTCCATCATTGCCTGGGAGCCGCTCTGGCCCGCCTCGAACTGCGCACGGTC
TACACCGCCCTGTGGCGGCGTTTTCCCACGCTGAGGCTCGCGGATCCCGATCGGGAGCCA
TCGTTCCGGTTGACCACTCCCGCGTATGGATTGACCAGTCTGATGGTCGCCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
 [20-398]  1.60000000000002e-92 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
 [5-398]  2.99998750445706e-86 SSF48264
SSF48264   Cytochrome_P450
 [217-371]  4.2e-16 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
 [91-102]  1.90000694315261e-51 PR00359 [138-154]  1.90000694315261e-51 PR00359 [155-170]  1.90000694315261e-51 PR00359 [192-214]  1.90000694315261e-51 PR00359 [271-282]  1.90000694315261e-51 PR00359 [289-316]  1.90000694315261e-51 PR00359 [317-332]  1.90000694315261e-51 PR00359 [338-347]  1.90000694315261e-51 PR00359 [347-358]  1.90000694315261e-51 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
 [340-349]  PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit