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CDS information : Acla_00050


close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction4,867 / 4,082 / - [in whole cluster]
4,867 / 4,082 / - [in contig]
Locationcomplement(4082..4867) [in whole cluster]
complement(4082..4867) [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)AknA
Gene
Gene (GenBank)aknA
EC number
Keyword
Note
Note (GenBank)
  • polyketide ketoreductase
Reference
ACC
PmId
[12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002)
Related Reference
ACC
Q8VWB5
PmId
[8157619] Nucleotide sequence of the aknA region of the aklavinone biosynthetic gene cluster of Streptomyces galilaeus. (J Bacteriol. , 1994)
ACC
Q56166
NITE
Jado_00110
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
ACC
Q54812
NITE
Adria_00250
PmId
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [AknA]
MPPASERVAIVTGATSGIGLAVARSLAEGGARVFICARDGDRVAHTVKELREAGHDVDGA
SCDVRDTARVRAFVQEARDRFGPVDALVNNAGRSGGGHTAQIADELWLDVIETNLNSVFR
MTREVLTTGGMLERGAGRIVNIASTGGKQGVALGAPYSASKHGVVGFTKALGLELAKTGI
TVNAVCPGYVETPMAERVRQGYAGAWDITEDEVLERFEAKIPLGRYSTPDEVAGLVGYLL
SSTAASITAQAMNVCGGLGNY
selected fasta
>putative polyketide C-9 ketoreductase [AknA]
ATGCCGCCTGCATCAGAACGCGTCGCCATCGTCACGGGTGCCACCAGCGGCATCGGTCTC
GCCGTGGCCAGGAGCCTGGCCGAAGGCGGTGCGCGGGTCTTCATCTGCGCCCGCGACGGC
GACCGGGTCGCCCACACCGTCAAGGAGTTGCGCGAGGCGGGCCACGACGTCGACGGCGCC
TCCTGCGACGTACGCGACACCGCGCGGGTACGCGCCTTCGTCCAGGAGGCCAGGGACCGG
TTCGGGCCCGTCGACGCCCTGGTGAACAACGCCGGCCGCAGCGGCGGCGGGCACACCGCG
CAGATTGCCGACGAGTTGTGGCTCGACGTGATCGAGACCAACCTCAACAGCGTCTTCCGG
ATGACCCGCGAGGTGCTGACGACCGGCGGGATGCTGGAGCGCGGCGCCGGCCGGATCGTC
AACATCGCCTCGACGGGCGGCAAGCAGGGCGTGGCCCTGGGGGCGCCGTACTCGGCGTCG
AAGCACGGCGTGGTCGGCTTCACCAAGGCCCTGGGCCTCGAGCTGGCGAAGACGGGCATC
ACCGTCAACGCGGTCTGCCCGGGTTACGTCGAGACACCGATGGCCGAGCGGGTGCGCCAG
GGATACGCCGGCGCCTGGGACATCACCGAGGACGAGGTGCTGGAGAGGTTCGAGGCGAAG
ATCCCCCTCGGCCGCTACTCCACGCCGGACGAGGTCGCCGGACTCGTCGGCTACCTGCTC
TCCTCGACGGCCGCGTCGATCACCGCCCAGGCGATGAACGTCTGCGGCGGGCTCGGCAAC
TACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
 [82-93]  4.99999811956429e-14 PR00080 [137-145]  4.99999811956429e-14 PR00080 [157-176]  4.99999811956429e-14 PR00080
PR00080   SDRFAMILY
 [3-248]  2.00000075217457e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
 [8-175]  1.09999999999999e-37 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
 [8-25]  4.39999001237725e-48 PR00081 [82-93]  4.39999001237725e-48 PR00081 [131-147]  4.39999001237725e-48 PR00081 [157-176]  4.39999001237725e-48 PR00081 [178-195]  4.39999001237725e-48 PR00081 [222-242]  4.39999001237725e-48 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
 [6-258]  1.69999999999998e-82 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
 [144-172]  PS00061
PS00061   ADH_SHORT
SignalP
 [1-27]  0.891 Signal
Bacteria, Gram-positive   
 [1-27]  0.52 Signal
Bacteria, Gram-negative   
 [1-23]  0.727 Signal
Eukaryota   
TMHMM No significant hit