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CDS information : Ansam_00470


close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction87,642 / 88,808 / + [in whole cluster]
4,896 / 6,062 / + [in contig]
Location87642..88808 [in whole cluster]
4896..6062 [in contig]
TypeCDS
Length1,167 bp (388 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative 3-amino-5-hydroxybenzoic acid synthase/UDP-3-keto-D-glucose aminotransferase
putative AHBA synthase/UDP-3-keto-D-glucose aminotransferase
Product (GenBank)3-amino-5-hydroxybenzoic acid synthase
Geneasm43
Gene (GenBank)
EC number4.2.1.144
2.6.1.-
Keyword
  • AHBA
Note
  • bifunctional protein
Note (GenBank)
  • AHBA synthase; ORF6; PLP dependent dehydratase; K190 covalently binds PLP, similar to RifK in the rifamycin biosynthetic gene cluster
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
Related Reference
ACC
O52552
NITE
Rifam_00310
PmId
[9497318] 3-Amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics. (J Biol Chem. , 1998)
[10433690] Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase. (Biochemistry. , 1999)
[12207505] Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: the RifN protein specifically converts kanosamine into kanosamine 6-phosphate. (J Am Chem Soc. , 2002)
[21081954] The biosynthesis of 3-amino-5-hydroxybenzoic acid (AHBA), the precursor of mC7N units in ansamycin and mitomycin antibiotics: a review. (J Antibiot (Tokyo). , 2011)

close this sectionSequence

selected fasta
>putative 3-amino-5-hydroxybenzoic acid synthase/UDP-3-keto-D-glucose aminotransferase [3-amino-5-hydroxybenzoic acid synthase]
MGSSPDAGIDFPAWPQHDDAERAALLRALDQGQWWRVGGSEVDEFEREFAEYHGAGHALA
VTNGTHALELALQVLDVGPGTEVIVPAFTFISSSQAVQRLGAVAVPVDVDPDTYCLDVAA
AEDAVTSRTSAIMPVHMAGQFADMDRLDKLSASTGVPVVQDAAHAHGAHWRGKRVGELGS
IATFSFQNGKLMTAGEGGAVLFADQAQWEKAFVLHSCGRPKGDRGYFHLTSGSNFRMNEF
SAAVLRAQLGRLDSQIATRQARWPVLSALLAGIDGVVPQTVDPRSDRNPSYMAMFRMPGV
TEERRNAVVDELVRRGIPAFMAFRAVYRTQAFWETGAPDLTPEELAARCPVSEEITRDCV
WLHHRVLLGAEEQVRRLAAVVADVVAGA
selected fasta
>putative 3-amino-5-hydroxybenzoic acid synthase/UDP-3-keto-D-glucose aminotransferase [3-amino-5-hydroxybenzoic acid synthase]
ATGGGCAGTTCTCCGGACGCAGGAATCGACTTCCCGGCCTGGCCGCAGCACGACGACGCC
GAGCGCGCGGCCCTGCTGCGCGCGCTGGACCAGGGCCAGTGGTGGCGGGTCGGCGGCAGC
GAGGTCGACGAGTTCGAGCGCGAGTTCGCCGAGTACCACGGCGCGGGCCACGCGCTCGCC
GTCACCAACGGCACGCACGCGCTGGAGCTGGCCCTCCAGGTGCTCGACGTGGGCCCCGGC
ACGGAGGTGATCGTGCCCGCCTTCACGTTCATCTCGTCCTCGCAGGCCGTGCAGCGCCTC
GGCGCGGTCGCCGTGCCCGTGGACGTCGACCCCGACACCTACTGCCTGGACGTCGCCGCC
GCCGAGGACGCCGTCACCTCCCGCACCTCGGCGATCATGCCCGTGCACATGGCGGGCCAG
TTCGCCGACATGGACCGGCTCGACAAGCTGTCCGCGAGCACCGGCGTGCCGGTCGTGCAG
GACGCCGCCCACGCGCACGGCGCCCACTGGCGCGGCAAGCGGGTCGGCGAGCTCGGGTCG
ATCGCCACGTTCAGCTTCCAGAACGGCAAGCTGATGACGGCGGGCGAGGGCGGGGCCGTC
CTGTTCGCCGACCAGGCCCAGTGGGAGAAGGCGTTCGTCCTGCACAGCTGCGGCAGGCCC
AAGGGCGACCGCGGCTACTTCCACCTCACCTCGGGCTCGAACTTCCGGATGAACGAGTTC
TCCGCCGCCGTGCTGCGCGCCCAGCTCGGCAGGCTGGACTCGCAGATCGCGACCAGGCAG
GCCCGCTGGCCGGTGCTGTCCGCGCTGCTGGCGGGGATCGACGGCGTCGTGCCGCAGACC
GTCGACCCGCGCAGCGACCGCAACCCGAGCTACATGGCGATGTTCCGGATGCCCGGCGTC
ACCGAGGAGCGGCGCAACGCGGTGGTGGACGAGCTGGTGCGGCGCGGGATCCCGGCCTTC
ATGGCGTTCCGGGCGGTCTACCGCACCCAGGCGTTCTGGGAGACCGGCGCGCCCGACCTG
ACCCCCGAGGAGCTGGCCGCGCGGTGCCCGGTGTCGGAGGAGATCACCCGCGACTGCGTG
TGGCTGCACCACCGGGTGCTGCTGGGCGCCGAGGAGCAGGTGCGCCGGCTGGCCGCGGTC
GTGGCCGACGTGGTCGCGGGCGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
 [1-388]  PIRSF000390
PIRSF000390   PLP_StrS
 [17-380]  5.50000000000008e-119 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
 [13-250]  4.69999999999992e-75 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
 [251-386]  2.4e-58 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
 [7-388]  1.39999277195148e-101 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit