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CDS information : Asco_00110


close this sectionLocation

Organism
StrainATCC 14891 (=NBRC 13981)
Entry nameAscomycin
Contig
Start / Stop / Direction12,093 / 11,245 / - [in whole cluster]
12,093 / 11,245 / - [in contig]
Locationcomplement(11245..12093) [in whole cluster]
complement(11245..12093) [in contig]
TypeCDS
Length849 bp (282 aa)
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close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)FkbK
Gene
Gene (GenBank)fkbK
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • acyl CoA dehydrogenase
Reference
ACC
PmId
[10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000)
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)
Related Reference
ACC
Q8KUG1
NITE
Ansam_00150
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
ACC
Q9ADL9
NITE
Soraph_00040
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)

close this sectionSequence

selected fasta
>putative oxidoreductase [FkbK]
MDHQLIVLGAGVMGTGITTLALRHGVPVTLVDTSEEILTAARARIATELRTAQLVAGPAE
ADPGVLRTATSALPAIGTATAVIEAVVEDAEVKAEVLATVSAAAPGIPVITNTSSIPVDE
LADSVARPEDLLGTHFMNPSYLIPTVEVIRGPRTGEKAMAAAGDLLAALRRKPIVVRDAP
GFVTSRLLHPMINDAARIVQEGTASVEDVDALMTGCLGHPTGPLRTADLIGIDNLVDALW
VLHKRTGDERSRPCDLLLGKVREGHHGRKTGRGFYEYEKVFS
selected fasta
>putative oxidoreductase [FkbK]
GTGGACCACCAGCTGATCGTGCTCGGCGCGGGAGTGATGGGCACCGGCATCACCACGCTC
GCGCTGCGCCACGGCGTCCCGGTGACACTCGTCGACACGTCCGAGGAGATCCTTACGGCG
GCGCGGGCGCGGATCGCCACCGAACTGCGGACGGCACAACTCGTGGCCGGGCCCGCCGAG
GCCGACCCCGGTGTGCTGCGGACGGCCACGTCCGCGCTGCCCGCGATCGGTACGGCCACC
GCGGTGATCGAGGCCGTCGTCGAGGACGCCGAGGTCAAGGCGGAGGTACTCGCCACGGTC
TCGGCAGCGGCGCCCGGTATCCCGGTCATCACGAACACGTCGTCGATCCCGGTTGACGAA
CTGGCCGACTCCGTGGCCCGGCCCGAGGACCTGCTCGGCACGCACTTCATGAACCCGTCG
TACCTGATCCCGACGGTCGAGGTGATCCGCGGACCCCGGACCGGTGAGAAGGCCATGGCC
GCCGCCGGGGACCTGCTCGCGGCGCTGCGGCGCAAGCCGATCGTCGTCCGGGACGCGCCC
GGCTTCGTCACGAGCCGGCTGCTCCACCCGATGATCAACGACGCGGCGCGGATCGTGCAG
GAGGGCACCGCGTCCGTCGAGGACGTCGACGCGCTCATGACGGGGTGCCTCGGCCATCCG
ACCGGGCCGCTGCGCACCGCGGACCTGATCGGCATCGACAACCTCGTCGACGCGCTGTGG
GTGCTGCACAAACGCACCGGCGACGAGCGCAGCCGCCCCTGTGACCTGCTGCTGGGCAAG
GTCCGCGAGGGACACCACGGCCGGAAGACCGGCCGGGGCTTCTACGAATACGAGAAGGTG
TTCTCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
 [181-277]  3.7e-30 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
 [6-178]  8.39999999999997e-45 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
 [181-278]  1.29999924468179e-30 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
 [183-278]  2.4e-30 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
 [4-182]  1.09999999999999e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
 [1-279]  4.60000044330866e-67 PIRSF000105
PIRSF000105   HCDH
SignalP No significant hit
TMHMM No significant hit