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CDS information : Balhi_00110


close this sectionLocation

Organism
StrainDSM 5908
Entry nameBalhimycin
Contig
Start / Stop / Direction34,782 / 35,957 / + [in whole cluster]
34,782 / 35,957 / + [in contig]
Location34782..35957 [in whole cluster]
34782..35957 [in contig]
TypeCDS
Length1,176 bp (391 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)P450 monooxygenase
Gene
Gene (GenBank)oxyA
EC number
Keyword
  • 2nd cross-linking
  • D-O-E ring
Note
Note (GenBank)
  • already deposited under Y16952
Reference
ACC
PmId
[10390204] Identification and analysis of the balhimycin biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908. (Antimicrob Agents Chemother. , 1999)
[11353482] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-New Insights into the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323). We thank M. Schierle, Dr. S. Stevanovic and Prof. H.-G. Rammensee for help with Edman degradation and J. Turner, Prof. B. List and Prof. D. Boger (La Jolla, USA) for discussions on the work. (Angew Chem Int Ed Engl. , 2001)
[12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001)
[15651041] The biosynthesis of vancomycin-type glycopeptide antibiotics--a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. (Chembiochem. , 2005)
[16730832] Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. (J Biotechnol. , 2006)

close this sectionSequence

selected fasta
>cytochrome P450 [P450 monooxygenase]
MFEESNALRGTEIHRRDRFAPGPELRSLMGEGTMSILQPPDSPGGRTGWLATGHDEVRQV
LGSDKFSAKLLYGGTVAGRIWPGFLNQYDPPEHTRLRRMVTSAFTVRRMQDFRPRIEQIV
QASLDAIEAAGGPVDFVPRFAWSVATTVTCDFLGIPRDDQADLSRALHASRSERSGKRRV
AAGNKYWTYMTEIAARARRDPGDDMFGAVVRDHGDAITDAELLGVAAFVMGAGGDQVARF
LAAGAWLMVEHPDQFALLREKPDTVPDWLNEVERYLTSDEKTTPRIAQEDVRIGDQLVKA
GDAVTCSLLAANRRKFPAPEDEFDITRERPVHVTFGHGIHHCLGRPLAEMVFRAAIPALA
QRFPKLRLAEPDREIKLGPPPFDVEALLLEW
selected fasta
>cytochrome P450 [P450 monooxygenase]
GTGTTCGAGGAGAGCAACGCCCTCCGGGGCACGGAAATACACCGGAGAGACCGGTTCGCT
CCGGGGCCGGAACTGCGCTCCCTGATGGGCGAGGGCACCATGTCCATCCTGCAGCCCCCG
GATTCCCCCGGCGGGCGGACCGGGTGGCTGGCCACCGGGCACGACGAGGTCCGGCAGGTC
CTCGGCTCGGACAAGTTCAGCGCCAAGCTGCTCTACGGCGGGACCGTGGCCGGCCGCATC
TGGCCGGGCTTCCTCAACCAGTACGACCCCCCGGAGCACACGCGCCTGCGCCGGATGGTG
ACGTCGGCGTTCACCGTCCGGCGGATGCAGGACTTCCGGCCGCGGATCGAGCAGATCGTC
CAGGCGAGCCTGGACGCCATCGAGGCCGCCGGTGGCCCGGTGGACTTCGTCCCCCGGTTC
GCCTGGTCCGTGGCGACGACGGTGACGTGCGACTTCCTCGGCATCCCGCGTGACGATCAG
GCGGACTTGTCGCGCGCCCTGCACGCCAGCCGGTCCGAACGGTCGGGCAAGCGGCGGGTG
GCGGCGGGGAACAAGTACTGGACGTACATGACCGAGATCGCGGCCCGCGCGCGCCGCGAT
CCCGGTGACGACATGTTCGGCGCGGTGGTGCGCGACCACGGCGACGCGATCACCGACGCG
GAACTGCTGGGCGTGGCCGCGTTCGTCATGGGCGCGGGCGGGGACCAGGTGGCCCGGTTT
CTCGCGGCGGGCGCGTGGCTGATGGTCGAGCACCCCGATCAGTTCGCGCTGCTGCGGGAA
AAGCCGGACACCGTCCCGGACTGGCTGAACGAGGTGGAGCGGTACCTCACCAGCGACGAG
AAGACCACTCCGCGCATCGCGCAGGAGGACGTGCGCATCGGTGATCAGCTCGTCAAGGCC
GGCGATGCCGTCACCTGCTCGCTGCTGGCGGCGAACCGCAGGAAGTTCCCCGCCCCGGAG
GACGAGTTCGACATCACCCGGGAACGGCCGGTGCACGTCACGTTCGGCCACGGCATCCAC
CACTGCCTCGGCAGGCCACTGGCCGAGATGGTGTTCCGGGCGGCGATTCCGGCGCTGGCA
CAACGCTTTCCCAAGCTGAGGCTGGCCGAGCCGGACCGCGAGATCAAGCTGGGGCCGCCG
CCGTTCGACGTGGAAGCCCTGCTGCTGGAGTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
 [270-363]  2e-08 PF00067
PF00067   p450
 [16-391]  2.1000026783403e-74 SSF48264
SSF48264   Cytochrome_P450
 [34-388]  7.79999999999999e-82 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
 [88-99]  4.19999771339582e-34 PR00359 [135-151]  4.19999771339582e-34 PR00359 [152-167]  4.19999771339582e-34 PR00359 [188-210]  4.19999771339582e-34 PR00359 [285-312]  4.19999771339582e-34 PR00359 [333-342]  4.19999771339582e-34 PR00359 [342-353]  4.19999771339582e-34 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
 [335-344]  PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit