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CDS information : Calm_00280


close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction32,797 / 31,589 / - [in whole cluster]
32,797 / 31,589 / - [in contig]
Locationcomplement(31589..32797) [in whole cluster]
complement(31589..32797) [in contig]
TypeCDS
Length1,209 bp (402 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative aminotransferase
Product (GenBank)CalE4
Gene
Gene (GenBank)calE4
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
Related Reference
ACC
P77444
PmId
[10739946] Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions. (J Biochem. , 2000)
[12941942] The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli. (J Biol Chem. , 2003)
Family/Domain
FD
IPR000192

close this sectionSequence

selected fasta
>putative aminotransferase [CalE4]
MAGHGPPLAYLDNAATTQKPRSVLDAVVDFYTTANSNIGRGFYELSRRATGRYEEAREVV
RRFVNAAHADEIVFTRGTTDAVNLLADTFAERIVGAGDDVVVTGMEHNSNLLPWRRLCER
RGARLRIVPSPAGGPVRPEDLAAVLGRRTRLVAVSHVSNVLGTVNPVRELVRVAHDRGVP
VVVDGAQAVAHLPVDVREIGADFYCFSGHKVYAPMGSGVLYGRRELLADLPPYQVGGGTV
KGVSHDAPVSYVPVPARLEAGTPDIGAAVGLAAALTWLDGLDREAVRAHDADLVRYAVDV
LCDVDRVRVVGDPAADPAGIVSIAVDGLHPYDVGGHLDAHGIAVRSGVHCASAFLDDLGL
LGTVRLSFAVYSTRGEVDRVAEALRTVRAGEWTTRYPDIRFR
selected fasta
>putative aminotransferase [CalE4]
GTGGCCGGGCACGGCCCGCCGCTGGCCTACCTCGACAACGCCGCGACGACGCAGAAGCCC
CGGTCGGTCCTGGACGCGGTGGTCGACTTCTACACCACCGCCAACAGCAACATCGGGCGC
GGCTTCTACGAGCTCAGCCGCCGGGCCACCGGCCGCTACGAGGAGGCCCGGGAGGTGGTC
CGGCGGTTCGTGAACGCCGCCCACGCCGACGAGATCGTCTTCACCCGGGGCACGACGGAC
GCGGTGAACCTGCTCGCCGACACCTTCGCCGAGCGGATCGTCGGCGCCGGTGACGACGTC
GTGGTCACCGGCATGGAGCACAACTCCAACCTGCTGCCGTGGCGGCGGCTCTGCGAGCGG
CGCGGGGCGAGGCTGCGGATCGTGCCGTCGCCGGCCGGCGGGCCGGTCCGGCCGGAGGAC
CTGGCCGCCGTCCTGGGCCGCCGGACCCGCCTCGTCGCGGTCAGCCACGTCTCCAACGTG
CTCGGCACCGTCAACCCGGTGCGGGAGCTGGTCCGGGTGGCGCACGACCGGGGCGTGCCC
GTGGTCGTCGACGGCGCGCAGGCGGTCGCGCACCTGCCGGTCGACGTGCGGGAGATCGGC
GCGGACTTCTACTGCTTCTCCGGCCACAAGGTGTACGCCCCGATGGGCTCCGGGGTGCTC
TACGGCCGGCGCGAGCTGCTCGCCGACCTGCCGCCGTACCAGGTGGGCGGCGGGACGGTC
AAAGGGGTCTCGCACGACGCTCCGGTGTCCTACGTGCCGGTTCCGGCGCGGCTGGAGGCC
GGCACCCCGGACATCGGCGCCGCGGTCGGGCTCGCCGCCGCGTTGACCTGGCTGGACGGG
CTCGACCGGGAGGCGGTGCGGGCACACGACGCGGACCTCGTCCGGTACGCCGTCGACGTC
CTGTGCGACGTCGACCGGGTGCGGGTCGTCGGCGACCCGGCCGCCGACCCGGCCGGCATC
GTCTCCATCGCCGTGGACGGCCTGCACCCGTACGACGTGGGCGGGCACCTCGACGCGCAC
GGCATCGCCGTACGGTCCGGCGTGCACTGCGCCAGCGCGTTCCTCGACGACCTGGGTCTG
CTCGGCACGGTGCGGCTCTCGTTCGCGGTCTACAGCACCCGTGGCGAGGTCGACCGGGTC
GCCGAGGCGCTGCGCACCGTGCGGGCGGGGGAGTGGACCACCCGGTACCCCGACATCCGG
TTCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR000192 Aminotransferase, class V/Cysteine desulfurase (Domain)
 [10-380]  1.69999999999998e-121 PF00266
PF00266   Aminotran_5
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
 [17-273]  2.70000000000002e-81 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
 [274-380]  3.7e-14 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
 [4-389]  2.1000026783403e-120 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit