close all open/close all

CDS information : Calm_00500


close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction59,091 / 58,105 / - [in whole cluster]
59,091 / 58,105 / - [in contig]
Locationcomplement(58105..59091) [in whole cluster]
complement(58105..59091) [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative drug resistance ABC transporter ATP-binding protein
Product (GenBank)CalT5
Gene
Gene (GenBank)calT5
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
Related Reference
ACC
P32010
NITE
Adria_00130
PmId
[1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991)
[9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997)
[15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005)
[19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
[25721665] Characterization of a novel domain 'GATE' in the ABC protein DrrA and its role in drug efflux by the DrrAB complex. (Biochem Biophys Res Commun. , 2015)

close this sectionSequence

selected fasta
>putative drug resistance ABC transporter ATP-binding protein [CalT5]
MTTNPALAIETRDLVKVFGQTRAVDGLDLVVRAGTIHGVLGPNGAGKTTAIKMLATLMRP
TSGTASVLGHDVVREAAEVRRRIGLTGQTMSVDEDMTGVQNLILAGRLQGLRHASAAARA
EQLMEAFDLTEVGGRLVKTFSGGQRRRIDVAASMVVTPELLFLDEPTTGLDPRSRSEVWE
MIRALVRDGGTVLLTTQYLDEADHLADELTLIDHGRIVAQGTPPELKASRAAGVLDVRLR
DPERRADAGALLAKAVGAAADLDSDPARLSVRVTDPDRAALALGELARAGIHVDDFTLGQ
PSLDTVFLALTGHSTVDASEEEEAEVRA
selected fasta
>putative drug resistance ABC transporter ATP-binding protein [CalT5]
ATGACAACCAATCCGGCCTTGGCCATCGAGACCCGCGATCTGGTGAAGGTCTTCGGCCAG
ACGCGCGCGGTCGACGGACTGGACCTGGTGGTGCGGGCCGGGACGATCCACGGGGTGCTG
GGCCCGAACGGCGCCGGCAAGACGACGGCCATCAAGATGCTCGCCACGCTGATGCGACCC
ACCTCCGGCACCGCGTCCGTGCTGGGGCACGACGTGGTCCGCGAGGCCGCCGAGGTCCGG
CGCCGCATCGGCCTCACCGGCCAGACCATGTCCGTCGACGAGGACATGACCGGCGTGCAG
AACCTGATCCTCGCCGGCCGCCTGCAGGGTCTGCGGCACGCGTCCGCGGCCGCGCGGGCG
GAGCAGTTGATGGAGGCGTTCGACCTCACCGAGGTCGGCGGCCGGCTGGTGAAGACCTTC
TCCGGCGGGCAGCGGCGGCGCATCGACGTGGCCGCGAGCATGGTGGTCACCCCCGAGCTG
CTGTTCCTCGACGAGCCGACCACCGGCCTCGACCCGCGCAGCCGCAGCGAGGTCTGGGAG
ATGATCCGGGCGCTGGTCCGGGACGGGGGCACCGTCCTGCTGACCACGCAGTACCTCGAC
GAGGCGGACCACCTCGCCGACGAGCTGACGCTCATCGACCACGGCCGCATCGTGGCGCAG
GGCACCCCGCCGGAGCTGAAGGCGAGCCGCGCCGCCGGCGTGCTCGACGTGCGGCTGCGT
GACCCCGAGCGCCGGGCCGACGCGGGCGCCCTGCTCGCCAAGGCCGTCGGCGCCGCCGCC
GACCTCGACTCCGATCCGGCGCGGCTGTCGGTGCGGGTGACCGACCCCGACCGGGCGGCG
CTGGCCCTGGGCGAGCTGGCGCGGGCCGGCATCCACGTCGACGACTTCACGCTCGGCCAG
CCCTCGCTCGACACGGTGTTCCTCGCCCTCACCGGTCACTCGACGGTCGACGCCAGCGAA
GAAGAGGAAGCAGAGGTACGGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
 [9-239]  PS50893
PS50893   ABC_TRANSPORTER_2
 [48-168]  4.1e-16 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
 [33-225]  1.20000117458134e-10 SM00382
SM00382   AAA
IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family)
 [16-312]  2.20000000000002e-107 TIGR01188
TIGR01188   DrrA
IPR017871 ABC transporter, conserved site (Conserved_site)
 [140-154]  PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit