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CDS information : Chalc_00240


close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction54,439 / 59,277 / + [in whole cluster]
54,439 / 59,277 / + [in contig]
Location54439..59277 [in whole cluster]
54439..59277 [in contig]
TypeCDS
Length4,839 bp (1,612 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase subunit
Gene
Gene (GenBank)chmGIV
EC number
Keyword
Note
Note (GenBank)
  • module 6 KS/AT/KR/ACP
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)

close this sectionPKS/NRPS Module

6 methylmalonyl-CoA
KS36..411
AT564..881
KR1162..1341
ACP1454..1524

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase subunit]
MATEHEQKLRDYLKRATTELHKATERLKEVEQRAHEPVAIVGMGCRFPGGASSPEELWDL
VAAETDAVSPFPVDRGWDVTGLYDPDPDAAGRAYVREGGFLHDAGEFDAGFFGISPREAL
AMDPQQRLLLETSWEALERAGIDPHTLRGTRTGVYMGAWNGGYAEGIPQPTAELEAQLLT
GGVVSFTSGRVSYLLGLEGPAVTVDTACSSSLVALHLAVRALRSGECDLALAGGATVMST
PDVFVRFSRQRGVAADGRCKAFSASADGFGPAEGVGVLAVERLSDAVRHGRRVLAVVRGS
AVNQDGASNGLTAPSGRAQALLIRRALADARLGVADVDVVEGHGTGTRLGDPIEAQALLA
TYGQRDAGRPLRLGSLKSNVGHTQAAAGVAGVIKMVMAMRHGVLPKTLHVDEPTAEVDWS
AGAVSLLREQEAWPEVGRLRRAAVSSFGVSGTNAHVVVEEAPVPEDGEAVGGGVPLAVVP
VVVSGRSAGAVAELAGRVSEVAASGRLVDVGLSSVVSRSVFEHRSVVLAGDSAELNAGLD
AVAGGVPSPGVVSGVASGEGGRSVFVFPGQGTQWAGMALGLWAESSVFAESMARCEAAFV
GLVDWRLSQVLSDGSALERVEVVQPASFAVMVSLAELWRSLGVVPDAVVGHSQGEIAAAV
VAGGLSLEDGARVVVLRARLIGRELAGRGGMASVALPVAVVEERLAGWAGRLGVAVVNGP
SATVVAGDVDAVAEFVAACEVEGVRARVLPVDYASHSAHVEDLKAELEQILAGIGPVTGG
IPFYSTSEAAQIDTAGLDAGYWFGNLRRPVRFQETVERLLADGFRVFVECGAHPVLTGAV
QETAESTGRQVCAVGSLRRDEGGLRRFLTSAAEAFVQGVEVSWPALFEGTGARTVDLPTY
PFQRRRYWLESRPPAAPIETAAASGIESWRYRVAWKSLSLSESSRLDGRWLLVVPETLDA
DGTRIAHDIQHALTTHGATVSRLTVDVTTTDRADLSARLTTTAAEDQGPLRGVLSLLSTD
ERQHPDHPGVDRATAGTMLLAQACGDLVVARGVEPRLWVVTRGAVAVSPAERPSSAGAQV
WGLGRCAALELPTRWGGMVDLPPAARDAGRHVRRLVRLLSETCAEDQVALRASGAYGRRL
LPASSPSVSVPRTAKSGYQPRGTVLVTGGTGALGGHLARWLARNGAEHIVLAGRRGEGAP
GAAELSAELKELGAEVTVAACDVADRNALRDMLESLPADRPLSGVFHAAGVPHSAPLAET
DVAGLAAVLPGKVVGARHLHELTREKELDAFVLYASGAGVWGSGGQSAYGAANAALDALA
EQRRAEGLPATSVSWGLWDGGGMAGERGEEFLTALGLRAMEPESAVAALEEALDRGDTCV
SVVDVDWSRFAESFTAFRPSPLIGELPGVRAVPDGSAGGPSDDLADAARHGGAADRGVPA
GLARATGDDRQDILLDLVRRHAAAVLGHPGPQHIEPDAGFRTLGFSSVTAVELANKLGAA
VGTKIPATFAFDHPNARAAASRLDVLLAASSDETAQEAEIRQALRTVPLARLRAAGLLDG
LLELAGLEAEPGLPGDVPDRGAATPDEESALAEVDGLDAEALVDLVLNQSDS
selected fasta
>polyketide synthase [polyketide synthase subunit]
ATGGCGACCGAACACGAGCAGAAGCTCCGCGACTACCTCAAGCGGGCCACCACCGAACTC
CACAAGGCCACGGAACGGCTGAAGGAGGTCGAACAACGCGCTCACGAGCCGGTTGCGATC
GTGGGGATGGGATGCCGGTTCCCGGGCGGGGCGTCCTCGCCTGAGGAGTTGTGGGACCTG
GTGGCGGCGGAGACGGACGCGGTCTCCCCCTTCCCGGTGGACCGGGGGTGGGACGTGACG
GGGCTGTACGACCCGGATCCGGACGCGGCAGGGCGTGCCTACGTCCGCGAGGGCGGGTTC
CTCCACGACGCGGGGGAGTTCGACGCGGGGTTCTTCGGAATCTCTCCGCGTGAGGCGTTG
GCGATGGATCCGCAGCAGCGGTTGCTGCTGGAGACGTCGTGGGAGGCGTTGGAGCGGGCG
GGCATCGATCCGCACACGCTGCGCGGCACGCGGACCGGGGTCTACATGGGTGCCTGGAAC
GGCGGATACGCCGAGGGGATTCCCCAACCCACGGCGGAACTGGAGGCCCAGCTCCTCACC
GGCGGCGTGGTGAGCTTCACCTCGGGCCGTGTGTCCTACCTCCTGGGTCTGGAGGGGCCG
GCGGTGACGGTGGACACGGCGTGTTCGTCGTCGCTGGTCGCGCTGCACCTGGCGGTGCGG
GCGCTGCGCAGTGGTGAGTGCGACCTCGCCCTCGCCGGCGGCGCGACGGTGATGTCGACG
CCCGACGTGTTCGTGCGCTTCTCCCGGCAGCGAGGAGTGGCCGCGGACGGTCGCTGCAAG
GCGTTCTCCGCGTCGGCCGACGGATTCGGACCGGCTGAGGGTGTGGGCGTGCTGGCGGTG
GAGCGGTTGTCGGATGCGGTGCGTCATGGGCGTCGGGTGCTGGCGGTCGTGCGTGGTTCC
GCGGTCAACCAGGACGGTGCGAGCAACGGACTGACGGCGCCGAGCGGACGAGCTCAGGCC
CTTCTGATTCGTCGAGCGTTGGCGGATGCGCGGTTGGGTGTGGCTGATGTGGATGTGGTG
GAGGGGCATGGGACGGGGACGCGTCTGGGTGATCCGATCGAGGCGCAGGCGTTGTTGGCG
ACGTATGGGCAGCGGGATGCGGGTCGGCCGTTGCGGCTTGGTTCGTTGAAGTCGAATGTG
GGGCATACGCAGGCGGCTGCCGGTGTGGCGGGCGTGATCAAGATGGTCATGGCGATGCGG
CACGGTGTCCTGCCGAAGACGCTGCACGTGGATGAGCCGACGGCGGAGGTGGACTGGTCG
GCCGGCGCGGTGTCTTTGCTGAGGGAGCAGGAGGCGTGGCCTGAGGTGGGGCGTCTGCGT
AGGGCTGCGGTGTCTTCGTTCGGTGTGAGTGGGACGAACGCGCATGTGGTGGTGGAGGAG
GCGCCGGTTCCGGAGGACGGGGAGGCGGTCGGGGGCGGTGTGCCTTTGGCTGTGGTGCCG
GTGGTGGTGTCGGGTCGTTCTGCGGGTGCGGTGGCGGAGCTGGCGGGCCGGGTCAGCGAG
GTGGCTGCGTCTGGTCGGTTGGTGGATGTGGGGTTGTCGTCGGTGGTGTCGCGGTCGGTG
TTCGAGCACCGGTCCGTGGTACTGGCGGGGGACTCTGCCGAGCTGAATGCCGGTTTGGAT
GCTGTGGCCGGTGGTGTGCCGTCGCCTGGTGTGGTGTCGGGTGTGGCGTCGGGTGAGGGT
GGCCGGTCGGTGTTCGTGTTCCCTGGTCAGGGGACGCAGTGGGCGGGGATGGCGCTCGGG
TTGTGGGCGGAGTCGTCGGTGTTCGCGGAGTCGATGGCGCGGTGTGAGGCGGCGTTCGTC
GGCTTGGTGGACTGGCGCTTGTCGCAGGTTTTGAGCGATGGGTCGGCGCTGGAGCGGGTG
GAGGTGGTGCAGCCGGCGTCGTTCGCGGTGATGGTGTCGCTTGCTGAGCTGTGGCGGTCG
TTGGGTGTGGTGCCGGATGCGGTGGTGGGGCATTCGCAGGGGGAGATCGCTGCTGCGGTG
GTGGCGGGTGGTTTGTCGCTGGAGGACGGGGCGCGTGTGGTGGTGTTGCGTGCGCGGTTG
ATCGGTCGTGAGCTGGCCGGGCGCGGTGGGATGGCGTCGGTGGCGCTGCCGGTCGCGGTG
GTGGAGGAGCGTCTGGCGGGGTGGGCGGGGCGTCTGGGTGTGGCGGTGGTCAACGGACCG
TCCGCCACGGTCGTCGCGGGTGATGTGGATGCGGTGGCGGAGTTCGTGGCCGCGTGCGAG
GTGGAGGGGGTTCGGGCGCGTGTTCTGCCGGTGGACTACGCCTCGCACTCGGCGCACGTG
GAGGACCTGAAAGCCGAGCTTGAACAGATTCTGGCCGGCATCGGCCCGGTGACCGGTGGG
ATCCCGTTCTATTCGACGTCCGAAGCCGCGCAGATCGACACGGCTGGTCTGGACGCGGGG
TACTGGTTCGGGAATCTGCGTCGGCCGGTGCGGTTCCAGGAGACGGTCGAGCGGTTGTTG
GCGGATGGTTTCCGGGTGTTCGTGGAGTGTGGCGCGCATCCGGTGCTGACGGGGGCGGTG
CAGGAGACCGCGGAATCCACCGGTCGCCAGGTGTGTGCGGTCGGATCCCTGCGTCGTGAC
GAGGGAGGTCTGCGCCGCTTCCTCACCTCGGCCGCGGAGGCATTCGTCCAAGGCGTGGAG
GTGTCCTGGCCGGCACTGTTCGAAGGCACCGGCGCCCGCACGGTCGACCTGCCCACCTAC
CCCTTCCAACGTCGGCGCTACTGGCTGGAGTCGCGCCCTCCCGCGGCGCCGATCGAGACT
GCCGCAGCCTCTGGCATCGAGAGCTGGCGCTACCGCGTGGCGTGGAAGAGCCTGTCGCTG
TCGGAGTCGTCGCGTCTTGACGGCCGGTGGCTGCTCGTCGTGCCCGAAACCCTGGACGCC
GACGGCACGCGGATCGCCCACGACATCCAGCACGCCCTCACCACCCACGGCGCCACGGTC
TCCCGTCTGACGGTCGACGTGACGACGACCGACCGCGCCGACCTGTCGGCGCGGCTCACC
ACCACCGCGGCCGAAGACCAGGGGCCTCTCCGGGGCGTCCTCTCCCTCCTGTCCACCGAT
GAACGGCAGCACCCGGATCATCCCGGTGTCGACCGTGCCACGGCGGGCACGATGCTGCTC
GCCCAGGCGTGCGGGGATCTGGTCGTGGCCCGGGGCGTGGAGCCGAGGCTGTGGGTCGTG
ACCCGCGGGGCGGTCGCGGTGTCCCCCGCCGAGCGTCCGTCGTCAGCCGGCGCCCAGGTC
TGGGGCCTGGGGCGCTGCGCGGCGCTCGAACTTCCCACTCGGTGGGGTGGGATGGTCGAC
CTTCCCCCGGCGGCCCGGGATGCTGGAAGGCACGTACGGCGGCTCGTGCGTCTGCTGTCG
GAGACCTGTGCGGAGGACCAGGTGGCGCTGCGTGCGTCGGGTGCGTACGGCCGCAGGCTG
CTGCCCGCGTCCAGCCCCTCCGTATCCGTCCCCCGGACCGCGAAGAGCGGCTACCAGCCG
CGCGGCACGGTGCTGGTGACCGGCGGAACCGGTGCCCTCGGTGGCCACTTGGCACGGTGG
CTGGCCCGCAACGGCGCCGAGCACATCGTTCTGGCCGGGCGTCGGGGCGAGGGTGCTCCA
GGAGCCGCGGAACTGTCGGCGGAGCTCAAGGAGCTGGGTGCGGAGGTCACCGTCGCGGCC
TGCGACGTGGCGGACCGGAACGCGTTGCGTGACATGCTGGAATCCCTGCCGGCCGACCGG
CCGCTGTCGGGGGTGTTCCACGCTGCCGGTGTCCCGCACTCGGCGCCGCTGGCCGAGACG
GATGTGGCGGGGCTCGCCGCCGTGCTCCCGGGGAAGGTCGTCGGGGCACGGCACCTGCAC
GAACTCACCAGGGAGAAGGAACTGGACGCGTTCGTGCTGTACGCGTCGGGCGCCGGGGTG
TGGGGGAGCGGCGGGCAGAGCGCGTACGGAGCCGCCAACGCCGCACTGGACGCGCTGGCC
GAACAGCGCCGGGCTGAGGGACTGCCCGCCACTTCGGTCTCCTGGGGCCTGTGGGACGGC
GGAGGCATGGCCGGCGAGCGAGGCGAGGAGTTCCTCACCGCCCTCGGCCTGCGGGCCATG
GAGCCCGAGTCGGCTGTCGCCGCCCTGGAGGAGGCCCTGGATCGTGGGGACACCTGCGTG
AGCGTGGTCGACGTCGACTGGTCCCGGTTCGCCGAGTCGTTCACCGCCTTCCGGCCCAGC
CCGCTGATCGGGGAGCTCCCCGGGGTACGTGCCGTGCCCGACGGATCGGCGGGCGGACCG
TCGGACGACCTCGCGGACGCTGCGCGGCACGGCGGGGCAGCCGACCGGGGTGTGCCTGCA
GGGCTCGCCCGGGCGACGGGCGACGACCGGCAGGACATCCTGCTCGATCTCGTACGCCGC
CATGCCGCCGCCGTCCTCGGTCACCCGGGACCGCAGCACATCGAGCCCGACGCCGGTTTC
CGGACCCTGGGGTTCAGTTCGGTCACCGCGGTGGAACTGGCCAACAAGCTCGGTGCGGCC
GTGGGAACGAAGATCCCCGCCACCTTCGCGTTCGACCACCCCAACGCCCGTGCCGCGGCG
TCCCGCCTCGACGTCCTGTTGGCGGCGTCGAGCGATGAGACCGCGCAGGAGGCGGAGATC
CGGCAGGCACTGCGGACTGTGCCGCTGGCCCGGCTGCGGGCTGCGGGGCTCCTCGACGGC
CTGCTCGAACTCGCCGGGCTGGAAGCGGAGCCCGGCCTGCCGGGCGACGTACCGGATCGC
GGTGCGGCCACGCCGGACGAGGAGTCCGCCCTGGCGGAAGTCGACGGCCTGGACGCCGAA
GCACTGGTCGACCTCGTCCTCAACCAGTCCGACTCCTGA
[6] KS36..411
[6] AT564..881
[6] methylmalonyl-CoA753..757
[6] KR1162..1341
[6] ACP1454..1524
[6] KS106..1233
[6] AT1690..2643
[6] methylmalonyl-CoA2257..2271
[6] KR3484..4023
[6] ACP4360..4572

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [556-684]  3.79999999999991e-71 G3DSA:3.40.366.10 [753-870]  3.79999999999991e-71 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
 [1482-1497]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1446-1538]  8.30001403791589e-17 SSF47336
SSF47336   ACP_like
 [1457-1520]  2.3e-09 PF00550
PF00550   PP-binding
 [1451-1524]  4.6e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1454-1524]  PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
 [1162-1340]  2.99999999999998e-65 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [36-286]  2.30000000000004e-99 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [294-411]  3.40000000000004e-46 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [564-881]  1.9e-102 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [5-29]  3.8e-09 PF08990
PF08990   Docking
 [6-36]  2.59999946718995e-09 SSF101173
SSF101173   Polyketide_synth_docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [562-862]  7.90003079443025e-64 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [687-752]  3.30000526807186e-18 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [38-298]  5.40000000000002e-92 G3DSA:3.40.47.10 [299-462]  2.1e-62 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [28-460]  7.0999832511256e-105 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1163-1347]  2.50000000000001e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [199-215]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [566-861]  1.09999184471405e-112 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1455-1527]  2.70000183580794e-23 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [38-463]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1162-1341]  7.90003079443025e-54 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit