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CDS information : Chart_00240


close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction21,796 / 22,581 / + [in whole cluster]
2,216 / 3,001 / + [in contig]
Location21796..22581 [in whole cluster]
2216..3001 [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)ChaZ protein
GenechaE
Gene (GenBank)chaZ
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
Related Reference
ACC
P16542
NITE
Grana_00250
PmId
[1400167] Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterologous genes from a different polyketide pathway. (J Bacteriol. , 1992)
ACC
P16544
NITE
Actino_00170
PmId
[2394677] Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: clarification of actinorhodin gene functions. (J Bacteriol. , 1990)
[15458634] The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding. (Structure. , 2004)
[18205400] Inhibition kinetics and emodin cocrystal structure of a type II polyketide ketoreductase. (Biochemistry. , 2008)
[18691223] Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. (FEMS Microbiol Lett. , 2008)

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [ChaZ protein]
MTDNIERTAIVTGASNGIGRAIAATLAAEGVRVHICGRDAETVEKTVTELRADRGQVSGQ
ACDVTKPDQVTALVADCVARYGPVDILVNNAGRPGGGITANIDNELWYATIDTNLNGVFL
MSKSVLNEGRMTERQNGRIINIASVWGKQGTIGGAPYAAAKHGVIGFSRCLALELAKTGI
TVNAVCPGYVETPMSVNVRACQAGIWQVDEEEALRRLASDIPIGRYSEPEEVAWMVSYLA
SSKAASVTGQALNVCGGFGVH
selected fasta
>putative polyketide C-9 ketoreductase [ChaZ protein]
ATGACGGACAACATTGAGCGGACAGCCATCGTCACCGGCGCGAGCAATGGGATCGGCCGG
GCGATCGCCGCCACACTGGCGGCGGAAGGCGTACGCGTACATATCTGCGGCAGGGACGCC
GAAACCGTCGAGAAGACGGTGACCGAACTGCGGGCGGACAGAGGCCAGGTCAGTGGCCAG
GCGTGCGACGTCACCAAGCCCGACCAGGTGACCGCGTTGGTGGCGGACTGCGTCGCACGG
TACGGACCGGTGGACATCCTGGTGAACAACGCCGGCCGGCCCGGCGGAGGAATCACCGCG
AACATCGACAACGAATTGTGGTACGCCACCATCGACACCAATCTCAACGGTGTTTTCCTG
ATGTCCAAATCCGTGCTGAACGAAGGGCGCATGACGGAGCGGCAGAACGGCCGCATCATC
AACATCGCCTCGGTGTGGGGAAAACAGGGAACGATCGGAGGCGCGCCCTACGCGGCCGCC
AAACACGGCGTCATCGGATTCAGCCGCTGCCTCGCGCTGGAACTCGCGAAGACCGGGATC
ACGGTCAATGCTGTGTGCCCCGGATATGTCGAGACCCCGATGTCGGTCAACGTACGCGCC
TGCCAGGCGGGCATCTGGCAGGTGGACGAGGAGGAGGCCCTGCGCCGCCTGGCCTCCGAC
ATCCCCATCGGCCGGTACAGCGAGCCGGAGGAGGTCGCCTGGATGGTCTCCTACCTCGCC
TCGTCCAAGGCCGCCTCGGTCACCGGCCAGGCGCTCAACGTGTGCGGCGGCTTCGGTGTC
CACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
 [82-93]  2.49999811956465e-14 PR00080 [137-145]  2.49999811956465e-14 PR00080 [157-176]  2.49999811956465e-14 PR00080
PR00080   SDRFAMILY
 [1-247]  7.99999145876753e-17 PIRSF000126
PIRSF000126   11-beta-HSD1
 [8-175]  2.50000000000001e-39 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
 [8-25]  4.10001399699315e-48 PR00081 [82-93]  4.10001399699315e-48 PR00081 [131-147]  4.10001399699315e-48 PR00081 [157-176]  4.10001399699315e-48 PR00081 [178-195]  4.10001399699315e-48 PR00081 [222-242]  4.10001399699315e-48 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
 [6-258]  1.20000000000001e-85 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
 [144-172]  PS00061
PS00061   ADH_SHORT
SignalP
 [1-23]  0.216 Signal
Bacteria, Gram-negative   
 [1-23]  0.343 Signal
Bacteria, Gram-positive   
 [1-23]  0.079 Signal
Eukaryota   
TMHMM No significant hit