close all open/close all

CDS information : Chlor_00070


close this sectionLocation

Organism
StrainA82846UV37B
Entry nameChloroeremomycin
Contig
Start / Stop / Direction31,325 / 32,500 / + [in whole cluster]
31,325 / 32,500 / + [in contig]
Location31325..32500 [in whole cluster]
31325..32500 [in contig]
TypeCDS
Length1,176 bp (391 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)PCZA363.6
GenecepE
oxyA
ORF7
Gene (GenBank)
EC number
Keyword
  • 2nd cross-linking
  • D-O-E ring
Note
Note (GenBank)
  • similar to P450 related oxidase/hydroxylase
Reference
ACC
PmId
[9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998)
[12596194] Vancomycin assembly: nature's way. (Angew Chem Int Ed Engl. , 2003)
Related Reference
ACC
O87673
NITE
Balhi_00110
PmId
[10390204] Identification and analysis of the balhimycin biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908. (Antimicrob Agents Chemother. , 1999)
[11353482] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-New Insights into the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323). We thank M. Schierle, Dr. S. Stevanovic and Prof. H.-G. Rammensee for help with Edman degradation and J. Turner, Prof. B. List and Prof. D. Boger (La Jolla, USA) for discussions on the work. (Angew Chem Int Ed Engl. , 2001)
[12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001)
[15651041] The biosynthesis of vancomycin-type glycopeptide antibiotics--a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. (Chembiochem. , 2005)
[16730832] Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. (J Biotechnol. , 2006)

close this sectionSequence

selected fasta
>putative cytochrome P450 [PCZA363.6]
MFAENNAVRGTEIHRREQFDPGPELRSLMAEGPMSILEADDPAEGRTGWLATGYDEIRQV
LGSDKFSAKLLYGGTVAGRIWPGFLNQYDPPEHTRLRRMLTSAFTVRRMQGFRPRIELIV
EATLDDIEATGGPVDFVPRFAWPIATTVTCDFIGIPRDDQADLSRALLASRSERTGKRRV
AAGNKFWTYMSQVAAQARRDPGDNMFGAVVREHGDAITDAELLGVAAFIIGAAGDQVARF
LAAGAWLIAEHPEQFAVLRDKPDTIPDWLNEVARYLTSDEKTTPRIALEDVYIGDQLVKA
GDAVTCSLLAANRRNFPAPEDQFDITRERPAHVTFGHGIHHCLGRPLAEMVFRTAITALT
RRFPTLRLAEPGREIKLGPPPFDVEVLLLDW
selected fasta
>putative cytochrome P450 [PCZA363.6]
GTGTTCGCGGAGAACAACGCCGTCCGGGGCACCGAGATACACCGGAGAGAGCAATTCGAT
CCGGGACCGGAGCTGCGTTCCCTGATGGCCGAGGGCCCCATGTCCATTTTGGAGGCCGAT
GATCCCGCCGAAGGGCGGACCGGGTGGCTTGCCACCGGGTACGACGAGATCCGGCAGGTT
CTCGGCTCGGACAAGTTCAGCGCCAAGCTGCTTTACGGCGGGACCGTGGCCGGCCGCATC
TGGCCGGGCTTCCTCAATCAGTACGACCCGCCGGAGCACACGCGCCTGCGCCGAATGCTG
ACATCCGCGTTCACTGTCCGGCGGATGCAGGGGTTCCGCCCGCGGATCGAGCTGATCGTC
GAGGCCACCCTTGACGACATCGAGGCCACCGGTGGCCCGGTGGACTTCGTGCCCCGGTTC
GCGTGGCCCATCGCGACGACGGTGACTTGCGACTTCATCGGCATTCCCCGTGATGATCAG
GCGGACTTGTCGCGCGCGCTGCTCGCCAGCCGGTCCGAACGGACGGGCAAACGGCGCGTG
GCGGCGGGAAACAAGTTCTGGACGTACATGAGCCAGGTCGCGGCTCAAGCGCGCCGCGAT
CCCGGTGACAACATGTTCGGCGCCGTGGTGCGCGAACACGGCGACGCGATCACCGACGCG
GAGCTGCTTGGGGTGGCCGCATTCATCATTGGCGCGGCTGGGGATCAGGTGGCTCGTTTC
CTCGCGGCGGGCGCGTGGCTGATCGCCGAGCATCCCGAGCAGTTCGCTGTGCTGCGGGAC
AAGCCGGACACCATCCCGGACTGGCTCAACGAGGTGGCCCGATACCTCACCAGCGACGAG
AAGACCACTCCGCGCATCGCGCTGGAGGACGTGTACATCGGTGATCAGCTCGTCAAGGCC
GGCGATGCCGTCACCTGCTCTTTGCTGGCTGCGAACCGCCGGAACTTCCCCGCCCCTGAG
GACCAGTTCGACATCACCCGGGAAAGGCCCGCGCACGTCACGTTCGGGCACGGCATCCAT
CACTGCCTTGGCCGTCCGTTGGCCGAGATGGTGTTCCGGACCGCGATCACGGCTCTGACG
CGCCGCTTTCCCACGCTGCGGCTGGCCGAACCGGGCCGTGAGATCAAGTTGGGCCCGCCG
CCGTTCGACGTCGAAGTCCTGCTGCTCGACTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
 [24-391]  1e-70 SSF48264
SSF48264   Cytochrome_P450
 [269-363]  1.3e-09 PF00067
PF00067   p450
 [15-376]  7.69999999999997e-81 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
 [88-99]  3.89999861795218e-35 PR00359 [135-151]  3.89999861795218e-35 PR00359 [152-167]  3.89999861795218e-35 PR00359 [188-210]  3.89999861795218e-35 PR00359 [267-278]  3.89999861795218e-35 PR00359 [285-312]  3.89999861795218e-35 PR00359 [333-342]  3.89999861795218e-35 PR00359 [342-353]  3.89999861795218e-35 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
 [335-344]  PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit