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CDS information : Chth_00320


close this sectionLocation

Organism
StrainDSM 40725
Entry nameChlorothricin
Contig
Start / Stop / Direction93,554 / 98,305 / + [in whole cluster]
93,554 / 98,305 / + [in contig]
Location93554..98305 [in whole cluster]
93554..98305 [in contig]
TypeCDS
Length4,752 bp (1,583 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)ChlA6
Gene
Gene (GenBank)chlA6
EC number
Keyword
Note
Note (GenBank)
  • type I PKS
Reference
ACC
PmId
[16793515] Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis. (Chem Biol. , 2006)

close this sectionPKS/NRPS Module

A11 malonyl-CoA
KS34..411
AT568..887
kr1225..1402
ACP1495..1564

close this sectionSequence

selected fasta
>polyketide synthase [ChlA6]
MTDQQKLVDYLKWVTADLHRTRQRLEELESGAREPIAIVGMSCRYPGGVRGPDDLWRLLA
EGTDAVSSFPSDRGWNTAELYHPDPGHPGTSYAREGGFLYDAAEFDAEFFGISPREAMAV
DPQQRLLLEASWEALEGARIDPATLRGSGTGVFTGVMYSDYGARLMGSDAQEYEGLVGNG
SAGSVASGRVAYTLGLEGPAVSVDTACSSSLVALHLACQALERGECSLALAGGVTVMATP
MLFVEFSRQRGMAPDGRCKSFAAAADGAGWAEGVGILALERLSDARRNGRTVLAVVRGSA
VNQDGASNGLTAPNGPAQQRLIQQALARAGVPAAEVDVVEGHGTGTTLGDPIEAQALLAT
YGKNRPANRPLWLGSIKSNIGHTQAAAGVAGIIKMVEAMRHEELPRTLHVDAPSPHVDWE
SGGVRLLTEPVPWPRGDRPRRAGVSSFGIGGTNAHVVLEEAPAAEDCAGEERQPVRRGGP
VPWVLSAKSAAALRAQADRLRAFVTASPDHDVTEIGRSLATTRTLLGHRAVLIAEDTEEF
VRRLGFLAEGQPAGNLVQGTASGGGVGFLFSGQGAQRLGMGRALHQAFPVFADALDEVCQ
RLEPLLGRSLKQVMWAARGSAEARLLDQTAHTQAALFAVEVALFRLLHHYGLRPDAVMGH
SVGELAAAHVAGVLSLTDACTLVAARGRLMQDLPPGGAMIAVEAAESEVATALDGLNAYA
SIAAVNGPRAVVVSGDEDVVRSVADGFAAQGRRTSRLRVSHAFHSPRMDPALREYDQVAR
SLTYHAPRIPVVSNVTGALATAGQLRDPAYWVAHARQPVRFADGVATLYAQGVTVFLELG
PDSVLAPLAAGCLPSDVPMPVPVLNARRPEAQTLPAALGHAHAHGAEVDWAVYFADYGTG
TVALPTYAFQHRRYWIDAVPRGTGAGEGAAQEARFWTAVERQDLDTLAADLHIGEEGLAA
LKALLPAFAGWRRRQRRHLGLGWEPVAGTPGTARAGTWLVAVPDDASAERVTRELDGPSL
TVVPVVVDVATASAADLAEALRTSAARTREVDGLLSLLAPVEGEREGVRLGNVTGDGASP
AAPAGRDPEAGPPALPADGDLPAALAATLALADALDASGLAVPLWIGTRGAMAVDRRDRA
PDPAQAMFWGLGQALAAERVHRRFGLVDLPADGDLLAGSAVGLARLLAHESADDQVALRP
SGTRVRRLGPGARPGGDDRRWQPAGTVLVCGSLLSGPGAEVARWVAGHEGARVLAVPPRS
EATAPERLAALRQELGDDLVVAGVDLTDRAAVAGLLASVPDELPLTAVVHLAAPQRDAAP
RPLDPGGVARASADVVAARHLDDLTRDLDLAAYVTVSSLTGVFGLPGFGDTGPAHAALDA
LAAGRRARGLPALSLLVAPWDESVTAGAGGPGVTGAAPEDITALLARAPHLGGHTQMVAD
FDWERLVPRLAGGRPGGLLLGVPEARQALDATPHAEGPKLRGLADVPVSERFDFLLDLIR
RQLAAVLGHAEPDAIADDSDFIALGLSSFTALELSTRLRTVGIEVSTADVYDHPSPAALA
RHLHARLGDADPSPASTDLLEAS
selected fasta
>polyketide synthase [ChlA6]
ATGACGGACCAACAGAAACTGGTCGACTACCTGAAATGGGTCACCGCCGACCTCCACCGG
ACGCGGCAGCGCCTGGAGGAGCTGGAGTCCGGGGCGCGGGAGCCCATCGCGATCGTGGGC
ATGAGCTGCCGCTATCCCGGGGGTGTGCGCGGGCCCGACGATCTGTGGCGGCTGCTGGCC
GAGGGCACCGACGCGGTGTCGTCGTTCCCCTCGGACCGGGGCTGGAACACCGCCGAGCTG
TACCATCCCGACCCCGGGCACCCGGGCACGTCGTACGCGCGCGAGGGCGGATTCCTGTAC
GACGCCGCCGAGTTCGACGCCGAGTTCTTCGGGATCAGCCCGCGCGAGGCGATGGCCGTC
GACCCGCAGCAGCGGCTGCTGCTGGAGGCGTCCTGGGAGGCGCTGGAGGGCGCCCGCATC
GATCCAGCGACCCTGCGCGGCAGCGGCACGGGTGTGTTCACGGGCGTGATGTACAGCGAC
TACGGGGCCCGGCTGATGGGCAGTGACGCCCAGGAGTACGAGGGCCTGGTCGGCAACGGC
AGCGCGGGCAGTGTCGCCTCGGGCCGGGTGGCCTACACTCTCGGTCTCGAAGGGCCCGCC
GTGTCGGTGGACACCGCCTGTTCGTCGTCGCTGGTGGCGCTGCATCTGGCGTGCCAGGCG
CTGGAGCGCGGCGAGTGCTCCCTGGCGCTGGCCGGGGGTGTGACCGTGATGGCGACGCCC
ATGCTGTTCGTGGAGTTCAGCCGGCAGCGCGGCATGGCCCCGGACGGCCGCTGCAAGTCG
TTCGCGGCGGCCGCGGACGGCGCGGGATGGGCCGAGGGCGTGGGCATACTCGCGCTGGAG
CGGCTGTCGGACGCCCGGCGCAACGGCCGTACGGTGCTCGCGGTGGTGCGCGGCAGCGCG
GTCAACCAGGACGGGGCGAGCAACGGTCTGACGGCACCCAACGGGCCGGCGCAGCAGCGG
CTCATCCAGCAGGCCCTCGCCCGGGCCGGGGTGCCGGCCGCCGAGGTCGACGTGGTGGAG
GGCCATGGCACCGGCACGACGCTCGGCGATCCCATCGAGGCGCAGGCGCTGCTGGCGACG
TACGGGAAGAACCGGCCGGCAAACCGGCCGCTGTGGCTGGGCTCGATCAAGTCGAACATC
GGGCACACGCAGGCCGCCGCCGGGGTCGCCGGCATCATCAAGATGGTCGAGGCGATGCGG
CACGAGGAGCTGCCGCGGACCCTGCACGTGGACGCGCCGTCGCCGCATGTGGACTGGGAG
TCGGGCGGGGTACGGCTGCTGACCGAGCCGGTGCCGTGGCCGCGCGGTGACCGCCCCCGG
CGGGCCGGGGTGTCCTCGTTCGGGATCGGCGGCACCAACGCGCATGTCGTCCTGGAGGAG
GCGCCCGCCGCCGAGGACTGCGCTGGGGAGGAGCGGCAGCCCGTCCGGCGCGGTGGTCCC
GTCCCCTGGGTCCTGTCCGCGAAGAGCGCTGCCGCGCTGCGGGCCCAGGCCGACCGGCTG
CGGGCCTTCGTCACCGCCTCCCCGGACCACGACGTCACCGAGATCGGCCGCTCTCTGGCC
ACCACGCGCACCCTGCTCGGCCATCGGGCGGTCCTCATCGCCGAGGACACCGAGGAGTTC
GTGCGGCGCCTGGGCTTCCTCGCCGAGGGACAGCCGGCCGGGAACCTGGTGCAGGGCACG
GCATCGGGGGGCGGCGTCGGGTTCCTGTTCTCCGGGCAGGGCGCGCAGCGGCTCGGCATG
GGCCGCGCGCTGCACCAGGCGTTCCCGGTGTTCGCCGACGCGCTGGACGAGGTGTGCCAG
CGCCTCGAACCGCTGCTCGGCCGGTCGTTGAAGCAGGTGATGTGGGCGGCGCGGGGCAGC
GCCGAGGCCCGGCTCCTTGACCAGACCGCACACACGCAGGCCGCGCTGTTCGCCGTGGAG
GTGGCGCTGTTCCGGCTGCTGCACCACTACGGGCTGCGGCCGGACGCGGTGATGGGCCAT
TCCGTCGGCGAACTGGCCGCCGCGCATGTCGCCGGCGTGCTGTCTCTGACCGACGCGTGC
ACGCTGGTGGCCGCCCGCGGCCGGCTCATGCAGGACCTCCCCCCGGGCGGCGCCATGATC
GCCGTGGAGGCGGCGGAGAGCGAGGTCGCCACCGCGCTCGACGGGCTCAACGCCTACGCG
TCCATCGCGGCGGTGAACGGGCCGCGGGCCGTCGTCGTCTCCGGCGACGAGGACGTCGTG
CGGTCGGTGGCCGACGGCTTCGCCGCGCAGGGCCGCCGGACGAGCAGGCTGCGCGTCTCG
CACGCCTTCCACTCACCACGCATGGACCCGGCGCTGCGCGAGTACGATCAGGTGGCCCGG
TCGCTGACTTATCACGCGCCGCGCATCCCGGTCGTGTCGAACGTGACCGGGGCGCTCGCC
ACCGCCGGCCAGCTGCGCGACCCCGCCTACTGGGTGGCGCACGCCCGGCAGCCCGTACGG
TTCGCCGACGGGGTGGCCACTCTGTATGCCCAAGGGGTCACCGTCTTCCTGGAGTTGGGT
CCGGACTCGGTGCTGGCGCCGCTGGCCGCCGGATGCCTCCCCTCGGACGTGCCGATGCCC
GTGCCGGTGCTGAACGCGCGGCGGCCCGAGGCCCAGACGCTGCCGGCCGCGCTGGGGCAC
GCCCATGCGCACGGGGCGGAGGTGGACTGGGCCGTGTACTTCGCGGACTACGGCACGGGT
ACGGTCGCCCTTCCCACGTACGCCTTCCAGCACCGGCGGTACTGGATCGACGCGGTGCCG
CGCGGCACGGGAGCGGGCGAAGGCGCGGCGCAGGAGGCGCGGTTCTGGACCGCCGTCGAG
CGGCAGGACCTGGACACGCTCGCCGCGGACCTGCACATCGGTGAGGAGGGGCTGGCCGCG
CTCAAGGCCCTGCTGCCCGCGTTCGCGGGGTGGCGGCGCAGGCAGCGGCGGCATCTCGGG
CTCGGCTGGGAGCCGGTCGCCGGCACGCCCGGCACGGCCCGGGCGGGCACCTGGCTGGTG
GCCGTGCCGGACGATGCCTCCGCCGAGCGCGTGACCCGCGAACTGGACGGGCCGTCCCTC
ACGGTGGTGCCGGTGGTCGTCGATGTCGCCACGGCATCGGCAGCGGACCTGGCCGAGGCC
CTGCGCACGTCGGCCGCCCGGACACGGGAGGTGGACGGCCTTCTGTCCCTGCTCGCGCCG
GTCGAGGGTGAGCGGGAGGGGGTGCGGCTGGGGAACGTGACGGGCGACGGGGCTTCCCCG
GCGGCACCGGCCGGCCGGGACCCGGAGGCCGGGCCGCCGGCGCTGCCGGCCGACGGTGAC
CTGCCCGCCGCGCTCGCCGCCACGCTCGCGCTGGCCGACGCCCTCGACGCGTCGGGGCTC
GCGGTCCCCCTGTGGATCGGCACGCGGGGTGCGATGGCCGTGGACCGGCGGGATCGCGCG
CCCGACCCCGCGCAGGCCATGTTCTGGGGCCTCGGGCAGGCCCTCGCCGCCGAGCGGGTG
CATCGCCGCTTCGGACTCGTCGACCTCCCGGCGGACGGTGATCTCTTGGCGGGCTCGGCG
GTCGGGCTGGCCCGGCTGCTGGCCCATGAGAGCGCCGACGACCAGGTGGCGCTGCGTCCG
TCGGGCACCCGGGTGCGGCGGCTCGGGCCGGGCGCCCGCCCCGGCGGTGACGACCGACGG
TGGCAGCCGGCCGGGACCGTGCTGGTCTGCGGCTCGCTGCTCTCCGGTCCGGGGGCCGAG
GTCGCCCGCTGGGTGGCCGGTCACGAGGGCGCCCGGGTCCTGGCCGTACCGCCCCGTTCC
GAGGCGACCGCCCCGGAACGGCTGGCGGCGCTACGGCAGGAGTTGGGCGACGATCTGGTG
GTCGCCGGCGTCGATCTCACCGACCGGGCGGCCGTCGCCGGCCTGCTCGCCTCGGTGCCC
GACGAACTGCCGCTGACGGCGGTGGTGCATCTCGCCGCACCGCAGCGGGACGCGGCGCCG
CGTCCGCTGGACCCGGGGGGTGTGGCGCGGGCCTCGGCCGATGTGGTCGCCGCCCGGCAC
CTGGACGATCTCACCCGCGACCTGGATCTGGCCGCGTACGTCACCGTCTCGTCCCTGACG
GGTGTCTTCGGGCTGCCCGGCTTCGGCGACACGGGACCCGCCCATGCCGCGCTGGACGCC
CTCGCGGCGGGCCGCAGGGCCCGGGGGCTGCCCGCGCTGTCCCTGCTGGTGGCCCCCTGG
GACGAGTCCGTCACCGCCGGCGCGGGCGGCCCCGGGGTGACGGGCGCGGCGCCCGAGGAC
ATCACCGCGCTGCTGGCCCGCGCCCCGCACCTCGGGGGCCACACGCAGATGGTGGCCGAT
TTCGACTGGGAGCGTCTGGTGCCGCGCCTCGCGGGCGGCCGGCCGGGCGGGCTGCTGCTC
GGGGTGCCGGAGGCCCGGCAGGCCCTGGACGCCACCCCGCACGCGGAGGGACCAAAGCTG
CGCGGGCTCGCCGATGTGCCCGTGTCGGAGCGCTTCGACTTCCTCCTCGATCTGATCCGC
CGTCAGCTCGCTGCGGTGCTGGGCCATGCCGAGCCGGACGCGATCGCGGACGACAGCGAC
TTCATCGCCCTCGGCCTGTCGTCGTTCACCGCCCTGGAACTGAGCACCCGGCTGCGCACG
GTGGGCATCGAGGTGTCCACGGCGGACGTCTACGACCATCCGAGCCCGGCCGCCCTCGCC
CGGCATCTGCACGCCCGACTCGGCGACGCCGACCCCTCGCCGGCGTCCACGGACCTTCTG
GAGGCCTCATGA
[11] KS34..411
[11] AT568..887
[11] malonyl-CoA761..765
[11] kr1225..1402
[11] ACP1495..1564
[11] KS100..1233
[11] AT1702..2661
[11] malonyl-CoA2281..2295
[11] kr3673..4206
[11] ACP4483..4692

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [560-693]  7.59999999999998e-77 G3DSA:3.40.366.10 [761-874]  7.59999999999998e-77 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR009081 Acyl carrier protein-like (Domain)
 [1498-1563]  1.1e-11 PF00550
PF00550   PP-binding
 [1493-1569]  5.30000000000001e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1487-1581]  2.10000267834031e-18 SSF47336
SSF47336   ACP_like
 [1495-1564]  PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
 [1225-1402]  1.1e-28 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [34-285]  6.7999999999999e-96 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [293-411]  9.9e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [568-887]  1.6e-62 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [1-27]  7.09999999999999e-14 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [565-871]  1.19999063421924e-71 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [695-760]  2.89999717862184e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [33-296]  3.90000000000001e-87 G3DSA:3.40.47.10 [297-463]  3.60000000000002e-66 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [26-463]  2.0000139277205e-102 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1226-1400]  4.5e-13 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [198-214]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [569-868]  5.79999653854781e-123 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1496-1567]  2.800005042611e-22 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [36-463]  SM00825
SM00825   PKS_KS
SignalP No significant hit
TMHMM No significant hit