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CDS information : Conca_00100


close this sectionLocation

Organism
StrainATCC 27449 (=NBRC 13477)
Entry nameConcanamycin A
Contig
Start / Stop / Direction77,034 / 77,906 / + [in whole cluster]
77,034 / 77,906 / + [in contig]
Location77034..77906 [in whole cluster]
77034..77906 [in contig]
TypeCDS
Length873 bp (290 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)methoxymalonate biosynthesis protein
Gene
Gene (GenBank)
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • ORF1*
Reference
ACC
PmId
[16207901] Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. (Microbiology. , 2005)
Related Reference
ACC
Q8KUG1
NITE
Ansam_00150
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
ACC
Q9ADL9
NITE
Soraph_00040
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)

close this sectionSequence

selected fasta
>putative oxidoreductase [methoxymalonate biosynthesis protein]
MSENNAGDAPNAHRLTILGAGVMGVGIATLAVGHGVPVALIDVDQAKRAEAEARIAHELR
LAGLMGALPDDVPTGELVISDSLSDAAGSTAVIEAVTEISELKEKVIAEVSVIVSPGTLL
VSNTSSIPIDELASAAARPAELVGTHFMNPSYLIRTVEVIRGARTGEAAMDSVRALLTTL
RRRPVVVRDAPGFVTSRVLHPMINDAARVVQEGTATAEDVDALMQGCLGHATGPLRTADL
IGIDNLVDSLNVLYERTGDSGCRPCDLLLEKVRAGDIGRKSGRGFYEYEG
selected fasta
>putative oxidoreductase [methoxymalonate biosynthesis protein]
ATGTCGGAAAACAACGCGGGTGACGCTCCGAACGCACATCGCCTCACCATCCTGGGCGCC
GGCGTCATGGGCGTGGGCATCGCCACCCTGGCGGTCGGCCACGGCGTACCGGTGGCCCTC
ATCGACGTCGACCAGGCGAAACGCGCGGAGGCGGAGGCCAGGATCGCCCACGAACTGCGG
CTCGCGGGGCTGATGGGCGCGCTGCCGGACGACGTCCCCACCGGGGAACTCGTCATCAGT
GATTCCTTGTCCGACGCGGCCGGTTCCACCGCGGTCATCGAGGCCGTGACAGAAATATCG
GAGCTCAAGGAGAAAGTGATCGCGGAGGTTTCCGTCATCGTTTCACCTGGTACTCTCCTG
GTGTCGAACACGTCGTCCATTCCCATTGACGAATTGGCCTCGGCGGCCGCGCGTCCGGCG
GAACTCGTCGGCACGCATTTCATGAACCCGTCCTATCTGATCCGCACGGTCGAGGTCATC
CGCGGCGCCCGCACCGGCGAGGCCGCCATGGACTCCGTACGGGCCCTGCTCACCACCCTG
CGCCGTCGGCCCGTCGTGGTCCGGGACGCCCCCGGCTTCGTGACCAGCCGGGTCCTGCAC
CCGATGATCAACGACGCGGCCCGGGTGGTACAGGAGGGAACCGCGACCGCCGAGGACGTC
GACGCCCTCATGCAGGGCTGCCTGGGGCACGCCACAGGTCCGTTGCGCACCGCGGACCTC
ATCGGCATCGACAACCTCGTCGACTCGCTGAACGTGCTGTACGAGCGCACCGGTGACAGT
GGCTGTCGTCCGTGCGACCTGCTGCTGGAGAAGGTGCGGGCAGGCGACATCGGCCGGAAG
TCCGGCCGTGGTTTCTACGAATACGAGGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
 [192-288]  7.20000000000003e-29 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
 [15-189]  3.89999999999997e-46 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
 [191-290]  2.800005042611e-29 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
 [194-289]  3.39999999999999e-28 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
 [10-193]  3.09999999999998e-42 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
 [1-289]  5.40001756046195e-59 PIRSF000105
PIRSF000105   HCDH
SignalP
 [1-1]  Signal-anchor
Eukaryota   
TMHMM
 [15-37]  Transmembrane (o-i)
Transmembrane 1