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CDS information : Conca_00120


close this sectionLocation

Organism
StrainATCC 27449 (=NBRC 13477)
Entry nameConcanamycin A
Contig
Start / Stop / Direction78,174 / 79,289 / + [in whole cluster]
78,174 / 79,289 / + [in contig]
Location78174..79289 [in whole cluster]
78174..79289 [in contig]
TypeCDS
Length1,116 bp (371 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)methoxymalonate biosynthesis protein
Gene
Gene (GenBank)
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • ORF3*
Reference
ACC
PmId
[16207901] Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. (Microbiology. , 2005)
Related Reference
ACC
Q9KIE5
NITE
Asco_00090
PmId
[14623185] Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis. (J Mol Biol. , 2003)
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)
ACC
Q8KUG3
NITE
Ansam_00170
PmId
[11960423] Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis. (J Am Chem Soc. , 2002)
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)

close this sectionSequence

selected fasta
>putative oxidoreductase [methoxymalonate biosynthesis protein]
MSEPLAEATASVTGLVGDRAGDWDRSGLLPVELLRKLGAAGQLCTGVPTEYGGLGWDSRT
SGEFTAHVGSLCSSLRSVMTSQGMAAWTVERLGDEAQRRALLPRLTSGELAAVGFSESQA
GSDLSGIDTRITVDGDTVVVDGHKVWMTAAHYADLLIVFGRMGDGAAAVVVPARAPGVHV
QRVADPLGCRAAGHAEVRLDSVRLPADSVLGGEGLSLPLLVTTALAYGRMSVAWGCVGIL
RACLAATTRHAASREQFGQPLARHQLVAKHLADLFVAEQVASRACEHASHRWDNGSPDMV
VATILAKHVSAGHAARGAASAVQVMASAGAQDGHPVARAYRDAKLMEIIEGSNEMCELLL
AQHALTSVEPT
selected fasta
>putative oxidoreductase [methoxymalonate biosynthesis protein]
GTGAGTGAGCCCCTCGCCGAGGCGACGGCATCGGTCACCGGCCTGGTCGGTGACCGGGCC
GGTGACTGGGACCGCTCGGGCCTGCTCCCCGTCGAACTGCTGAGAAAGCTCGGTGCCGCC
GGGCAGTTGTGCACCGGCGTGCCCACCGAGTACGGCGGCCTCGGCTGGGACAGCCGCACC
AGCGGAGAGTTCACCGCCCACGTCGGCAGCCTGTGCAGCTCCCTGCGCAGCGTCATGACC
TCCCAGGGCATGGCCGCCTGGACCGTCGAACGGCTCGGCGACGAGGCGCAGCGCCGCGCG
CTGCTGCCCCGCCTCACCTCGGGCGAACTGGCCGCTGTCGGCTTCAGCGAGAGCCAGGCC
GGCAGCGATCTGTCGGGCATCGACACCCGGATCACCGTCGACGGCGACACCGTCGTGGTC
GACGGCCACAAGGTGTGGATGACCGCTGCCCACTACGCCGACCTGCTCATCGTCTTCGGA
CGGATGGGCGACGGCGCCGCCGCCGTGGTCGTGCCCGCCCGGGCGCCGGGCGTCCACGTA
CAGCGGGTGGCCGACCCCCTGGGCTGCCGCGCCGCCGGACACGCCGAGGTCCGCCTCGAC
TCCGTACGCCTCCCCGCCGACAGCGTGCTGGGCGGCGAGGGCCTGTCCCTGCCGCTGCTC
GTCACCACCGCCCTGGCCTACGGGCGGATGTCGGTCGCCTGGGGATGCGTCGGAATCCTG
CGCGCCTGCCTGGCCGCGACGACCCGGCACGCCGCGTCCCGCGAGCAGTTCGGGCAGCCG
CTGGCCCGCCACCAGCTCGTCGCCAAGCACCTGGCCGACCTCTTCGTCGCGGAGCAGGTC
GCCTCCCGCGCCTGCGAACACGCCAGCCACCGCTGGGACAACGGGTCCCCCGACATGGTG
GTCGCCACCATCCTCGCCAAGCACGTCAGCGCCGGCCACGCGGCCCGCGGGGCCGCGTCC
GCCGTACAGGTCATGGCGTCGGCGGGAGCCCAGGACGGCCATCCGGTGGCCCGCGCCTAC
CGGGACGCCAAGCTGATGGAGATCATCGAGGGCAGCAACGAGATGTGCGAACTCCTGCTC
GCCCAACACGCGTTGACAAGTGTGGAGCCGACATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain)
 [221-365]  4.80000000000001e-31 PF00441
PF00441   Acyl-CoA_dh_1
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain)
 [112-162]  1.3e-13 PF02770
PF02770   Acyl-CoA_dh_M
 [112-203]  1.4e-28 G3DSA:2.40.110.10
G3DSA:2.40.110.10   Acyl_CoA_DH/ox_M
IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain)
 [19-109]  1.9e-14 PF02771
PF02771   Acyl-CoA_dh_N
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain)
 [204-366]  1.09999999999999e-39 G3DSA:1.20.140.10
G3DSA:1.20.140.10   AcylCoA_DH_1/2_C
 [213-366]  3.60000433965927e-32 SSF47203
SSF47203   AcylCoADH_C_like
IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain)
 [5-214]  8.5000302480906e-55 SSF56645
SSF56645   AcylCoA_dehyd_NM
IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain)
 [16-109]  1.99999999999999e-23 G3DSA:1.10.540.10
G3DSA:1.10.540.10   AcylCoA_DH/ox_N
SignalP No significant hit
TMHMM No significant hit