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CDS information : Dauno_00100


close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction19,105 / 20,175 / + [in whole cluster]
1,835 / 2,905 / + [in contig]
Location19105..20175 [in whole cluster]
1835..2905 [in contig]
TypeCDS
Length1,071 bp (356 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
ProductC-4 O-methyltransferase
Product (GenBank)carminomycin 4-O-methyltransferase
Gene
Gene (GenBank)dauK
EC number2.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7836284] Analysis of clustered genes encoding both early and late steps in daunomycin biosynthesis by Streptomyces sp. strain C5. (J Bacteriol. , 1995)
[9098063] In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. (J Bacteriol. , 1997)
Related Reference
ACC
Q06528
NITE
Adria_00330
PmId
[8509343] Cloning and sequencing of a gene encoding carminomycin 4-O-methyltransferase from Streptomyces peucetius and its expression in Escherichia coli. (J Bacteriol. , 1993)
[7601857] Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995)
[15273252] Crystal structure of a ternary complex of DnrK, a methyltransferase in daunorubicin biosynthesis, with bound products. (J Biol Chem. , 2004)

close this sectionSequence

selected fasta
>C-4 O-methyltransferase [carminomycin 4-O-methyltransferase]
MTAEPTVAARPQQIDALRTLIRLGSLHTPMVVRTAATLRLVDHILAGARTVKALAARTDT
RPEALLRLIRHLVAIGLLEEDAPGEFAPTEVGKLLADDHPAAQRAWHDLTQAVARADISF
TRLPEAIRSGRPTYESVYGKPFYEDLAGRPDLRASFDSLLACDQDVAFDAPAAAHDWTNV
RHVLDVGGGKGGFAAAIARRAPHVSATVLEMAGTVDTARSYLRDAGLSDRVDVVEGDFFE
PLPRRADAIILSFVLLNWPDHDAVRILTRCAEALEPGGRILIHERDDLHENSFNEQFTEL
DLRMLVFLGGALRTREKWDGLAASAGLVVEEVRQLPSPTIPYDLSLLVLAPASTGA
selected fasta
>C-4 O-methyltransferase [carminomycin 4-O-methyltransferase]
ATGACAGCCGAACCGACGGTCGCGGCCCGGCCGCAGCAGATCGACGCCCTCAGGACCCTG
ATCCGCCTCGGAAGCCTGCACACGCCCATGGTCGTCCGGACGGCTGCCACCCTGCGGCTC
GTCGACCACATCCTGGCGGGGGCCCGCACCGTGAAGGCCCTGGCGGCCAGAACCGACACG
CGGCCGGAAGCACTCCTGCGACTGATCCGCCACCTGGTGGCGATCGGCCTGCTCGAGGAG
GACGCACCGGGCGAGTTCGCCCCGACCGAGGTCGGCAAGCTGCTCGCCGACGACCACCCG
GCCGCTCAGCGTGCCTGGCACGACCTGACGCAGGCCGTGGCGCGTGCCGACATCTCCTTC
ACCCGCCTCCCCGAAGCCATCCGTAGCGGCCGCCCCACGTACGAGTCCGTCTACGGCAAG
CCGTTCTACGAAGATCTGGCCGGCCGCCCTGACCTGCGCGCGTCCTTCGACTCGCTGCTC
GCCTGCGACCAGGACGTCGCCTTCGACGCTCCGGCCGCCGCTCACGACTGGACGAACGTC
CGGCACGTGCTCGACGTGGGGGGTGGCAAGGGGGGGTTCGCCGCGGCCATCGCGCGCCGG
GCCCCACATGTGTCGGCCACCGTGCTGGAGATGGCGGGCACCGTGGACACCGCCCGCTCC
TACCTGAGGGACGCGGGCCTCTCCGACCGTGTCGACGTCGTCGAAGGGGACTTCTTCGAG
CCGCTGCCCCGCAGGGCGGACGCGATCATCCTCTCCTTCGTCCTCCTCAACTGGCCGGAT
CACGATGCTGTCCGAATCCTTACCCGCTGTGCCGAGGCCCTGGAGCCCGGCGGGCGGATC
CTGATCCACGAGCGCGACGACCTCCACGAGAACTCGTTCAACGAACAGTTCACCGAGCTC
GATCTGCGGATGCTGGTCTTCCTCGGCGGTGCCCTGCGCACCCGCGAGAAGTGGGACGGC
CTGGCCGCGTCGGCGGGCCTCGTGGTCGAGGAGGTACGGCAACTGCCTTCGCCGACCATC
CCGTACGACCTCTCGCTCCTCGTCCTCGCCCCCGCGTCCACCGGCGCTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001077 O-methyltransferase, family 2 (Domain)
 [85-322]  2.30000000000004e-67 PF00891
PF00891   Methyltransf_2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
 [23-91]  1.9e-11 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016461 O-methyltransferase, caffeic acid-type (Family)
 [1-334]  3.89999861795218e-13 PIRSF005739
PIRSF005739   O-mtase
SignalP
 [1-35]  0.269 Signal
Bacteria, Gram-positive   
TMHMM No significant hit