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CDS information : Dynm_00460


close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction59,389 / 58,388 / - [in whole cluster]
59,389 / 58,388 / - [in contig]
Locationcomplement(58388..59389) [in whole cluster]
complement(58388..59389) [in contig]
TypeCDS
Length1,002 bp (333 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative drug resistance ABC transporter ATP-binding protein
Product (GenBank)DynT5
GenedynT5
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • putative ABC transporter ATP-binding protein; daunorubicin resistance ABC transporter ATPase subunit; similar to CalT5 protein in GenBank Accession Number AAM70341
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
Related Reference
ACC
P32010
NITE
Adria_00130
PmId
[1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991)
[9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997)
[15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005)
[19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
[25721665] Characterization of a novel domain 'GATE' in the ABC protein DrrA and its role in drug efflux by the DrrAB complex. (Biochem Biophys Res Commun. , 2015)

close this sectionSequence

selected fasta
>putative drug resistance ABC transporter ATP-binding protein [DynT5]
MGLSKSFGKVVALNGLDVRLESGQVAAILGPNGAGKTTFLRMISTLTRPDGGTLRVCGID
VTAEPARVRQLIGLAGQHATVEPALTGRENLQMIARLFGQDRRTARASAEETLERLRLGE
AGDRLVRTYSGGMRRRLDLGASLVGRPQVLLLDEPTTGLDPQSRIELWDTIRALTTEGTD
VLLTTQYLEEADRLANTILIIDHGQVIASGTAKELKSQAGRDIVEIHVRDREDLDPAVRA
LTRIGAETPVVDHGSLMASVAVDSGPERLREALHEVSELGVHVEDVALRRPTLDEVFLSL
TGSTPAGEPRGAEPRSPESPAGIEPETVSRSSR
selected fasta
>putative drug resistance ABC transporter ATP-binding protein [DynT5]
GTGGGCCTCAGCAAGTCCTTCGGGAAGGTCGTCGCCCTCAACGGGCTCGACGTGCGCCTG
GAGTCCGGCCAGGTCGCCGCGATCCTCGGCCCCAACGGGGCCGGTAAGACCACCTTCCTG
CGCATGATCTCCACGCTCACCCGGCCGGACGGCGGCACGCTGCGGGTCTGCGGCATCGAC
GTGACCGCCGAGCCGGCCCGGGTCCGCCAGCTCATCGGCCTCGCCGGGCAGCACGCGACG
GTCGAGCCCGCGCTGACCGGGCGGGAGAACCTCCAGATGATCGCCCGGCTGTTCGGCCAG
GACCGGCGTACCGCCCGGGCCAGCGCCGAGGAGACGCTGGAGCGCCTGCGCCTCGGCGAG
GCCGGCGACCGGCTGGTCCGCACCTACTCCGGCGGCATGCGGCGCCGGCTCGACCTGGGC
GCCAGCCTGGTCGGCCGCCCGCAGGTGCTGCTGCTCGACGAGCCCACCACCGGGCTCGAC
CCGCAGAGCCGGATCGAGCTGTGGGACACCATCCGGGCGCTCACCACCGAGGGCACCGAC
GTGCTGCTCACCACGCAGTACCTGGAGGAGGCGGACCGGCTCGCCAACACCATCCTGATC
ATCGACCACGGGCAGGTGATCGCCTCCGGCACGGCCAAGGAGCTGAAGAGCCAGGCCGGC
CGCGACATCGTGGAGATCCACGTCCGCGACCGGGAGGATCTCGACCCGGCCGTGCGGGCG
CTGACCCGGATCGGCGCCGAGACGCCTGTCGTCGACCACGGCTCGCTGATGGCCTCGGTG
GCGGTCGACTCCGGTCCGGAGCGGCTCCGCGAGGCGTTGCACGAGGTCAGCGAGCTGGGC
GTGCACGTCGAGGACGTGGCCCTGCGCCGGCCCACGCTCGACGAGGTGTTCCTGTCGCTG
ACCGGGTCGACCCCGGCGGGCGAGCCCCGTGGCGCCGAGCCGCGGTCGCCCGAGTCCCCC
GCGGGCATCGAGCCCGAGACCGTGTCGAGGAGTTCCCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
 [37-157]  5.09999999999998e-19 PF00005
PF00005   ABC_tran
 [1-228]  PS50893
PS50893   ABC_TRANSPORTER_2
IPR003593 AAA+ ATPase domain (Domain)
 [22-238]  3.50000235434314e-12 SM00382
SM00382   AAA
IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family)
 [5-302]  1.10000000000001e-100 TIGR01188
TIGR01188   DrrA
IPR017871 ABC transporter, conserved site (Conserved_site)
 [129-143]  PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit