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CDS information : Gelda2_00120


close this sectionLocation

Organism
StrainNRRL 3602 (=NBRC 14620)
Entry nameGeldanamycin
Contig
Start / Stop / Direction61,897 / 63,534 / + [in whole cluster]
50,597 / 52,234 / + [in contig]
Location61897..63534 [in whole cluster]
50597..52234 [in contig]
TypeCDS
Length1,638 bp (545 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Productputative hydroxylase
Product (GenBank)GdmM
Gene
Gene (GenBank)gdmM
EC number
Keyword
Note
Note (GenBank)
  • similar to Rif19; putative FAD-dependent monooxygenase
Reference
ACC
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
Related Reference
PmId
[18600054] Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427. (J Microbiol Biotechnol. , 2008)
ACC
Q9AE02
NITE
Rifam_00520
PmId
[10430893] Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively. (Proc Natl Acad Sci U S A. , 1999)
[16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005)
ACC
P77397
PmId
[9603882] Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12. (J Bacteriol. , 1998)
ACC
Q06BM2
NITE
Gelda_00030
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)

close this sectionSequence

selected fasta
>putative hydroxylase [GdmM]
MTGKEAAVDTARETDSLEAEVLIVGYGPVGQLLSVLLAQRGRRVTVVERWPEPYRHPRAV
GFDSEAARLLASAGIGDSLDKFTEPARDHAWQNTKGETLIDHEVADRGHCTWPEALSAYQ
PALESALIEHGETLPPLRILRGYEAVGLADDGDHVTLTVVGPDGEKTDLTALWVVGCDGA
NSLVRTGVGTTMTDLDFSYDWLICDVRLHEHREFRPNNLEICDPARPRTAVSAGPGHRRY
EFMRVPADDPEHFGTVESAWELLRLFDVTPENGVLDRHAVYTFQARWAERWRTGRMVLAG
DSAHLMPPFAGQGMCSGFRDAANLAWKLDLVLGGHAAPTLLDTYTTERRAHVRHAVEMSV
GLGRVVCMADPAAAADRDAAMLAARKRNIGPSAARRSVVRPLVDGLLRQDGQGRPAPYAG
QAGPQWRVCRAGTTGLFDDVVGTGFVLLYAEDVFPALDARRLTFLDSIGTRLVRMVPADT
PPAALGPRDALDVEDRYLSYLSEMDALAVLVRPDFYLFGIAEDEGELLSLVDDLATQLSP
SPTPS
selected fasta
>putative hydroxylase [GdmM]
ATGACCGGGAAGGAGGCGGCAGTGGACACCGCGCGGGAAACGGACAGCCTCGAGGCCGAG
GTGCTGATCGTCGGCTACGGACCGGTGGGCCAGCTACTGTCGGTGCTACTGGCCCAGCGC
GGGCGGCGCGTGACGGTCGTGGAGCGCTGGCCGGAGCCGTACCGGCACCCCCGGGCGGTC
GGGTTCGACAGTGAGGCCGCGCGCCTTCTGGCCTCGGCCGGGATCGGCGACTCGCTCGAC
AAGTTCACCGAACCCGCGCGGGACCACGCCTGGCAGAACACGAAGGGCGAGACGCTGATC
GACCACGAGGTGGCCGACCGGGGGCACTGCACCTGGCCGGAGGCTTTGTCGGCGTATCAG
CCCGCCCTGGAGTCCGCGCTGATCGAGCACGGGGAGACGCTGCCGCCGCTGCGGATCCTG
CGCGGATACGAGGCGGTGGGACTCGCGGACGACGGCGACCATGTGACCTTGACCGTGGTC
GGCCCGGACGGGGAGAAGACGGACCTCACCGCGCTGTGGGTGGTCGGCTGCGACGGCGCG
AACAGCCTGGTAAGGACGGGCGTCGGCACCACCATGACGGACCTCGACTTCTCGTACGAC
TGGCTGATCTGCGATGTGCGGTTGCACGAGCACCGCGAGTTCCGGCCGAACAACCTGGAG
ATCTGCGATCCGGCGCGCCCCCGGACGGCGGTGTCCGCGGGTCCTGGCCACCGGCGGTAC
GAGTTCATGCGGGTGCCCGCGGACGACCCCGAACACTTCGGCACCGTGGAGAGCGCCTGG
GAGCTGCTGCGGCTGTTCGATGTGACGCCCGAGAACGGCGTTCTGGACCGGCACGCGGTC
TACACCTTCCAGGCCCGCTGGGCGGAGCGCTGGCGGACCGGACGGATGGTGCTGGCCGGG
GACTCGGCACACCTCATGCCGCCGTTCGCGGGGCAGGGCATGTGCTCCGGATTCCGTGAC
GCGGCCAATCTGGCCTGGAAACTGGACCTGGTCCTGGGCGGACACGCGGCGCCGACGCTG
CTGGACACCTACACCACCGAGCGGCGGGCACACGTGCGGCACGCGGTGGAGATGTCGGTG
GGCCTGGGCCGGGTGGTGTGCATGGCGGACCCGGCCGCGGCGGCGGACCGTGACGCGGCG
ATGCTGGCCGCGCGCAAACGCAACATCGGCCCGAGTGCCGCCCGCCGTTCCGTGGTGAGG
CCGCTCGTGGACGGGCTGCTACGGCAGGACGGTCAGGGCCGCCCGGCACCGTACGCCGGC
CAGGCGGGCCCCCAGTGGCGAGTGTGCCGCGCGGGAACCACCGGCCTGTTCGACGACGTG
GTGGGCACCGGTTTCGTCCTCCTCTACGCCGAGGACGTGTTCCCCGCGCTGGACGCGCGG
CGGCTGACATTCCTCGACAGCATCGGCACCCGACTGGTGCGCATGGTCCCCGCGGACACG
CCCCCGGCCGCCCTGGGGCCACGGGACGCGCTGGACGTGGAGGACCGGTACCTGTCCTAT
CTGTCGGAGATGGACGCGCTGGCGGTACTGGTACGCCCGGACTTCTACCTGTTCGGCATC
GCGGAGGACGAGGGCGAACTCCTCTCTCTCGTAGACGACTTGGCCACCCAGCTGAGCCCG
TCACCCACTCCTTCGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR002938 Monooxygenase, FAD-binding (Domain)
 [18-358]  6.90000000000001e-64 PF01494
PF01494   FAD_binding_3
IPR003042 Aromatic-ring hydroxylase-like (Domain)
 [20-42]  1.3000049540733e-33 PR00420 [170-185]  1.3000049540733e-33 PR00420 [293-308]  1.3000049540733e-33 PR00420 [308-324]  1.3000049540733e-33 PR00420 [326-344]  1.3000049540733e-33 PR00420 [344-360]  1.3000049540733e-33 PR00420
PR00420   RNGMNOXGNASE
SignalP No significant hit
TMHMM No significant hit