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CDS information : Gelda2_00130


close this sectionLocation

Organism
StrainNRRL 3602 (=NBRC 14620)
Entry nameGeldanamycin
Contig
Start / Stop / Direction66,360 / 64,312 / - [in whole cluster]
55,060 / 53,012 / - [in contig]
Locationcomplement(64312..66360) [in whole cluster]
complement(53012..55060) [in contig]
TypeCDS
Length2,049 bp (682 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative carbamoyltransferase
Product (GenBank)GdmN
Gene
Gene (GenBank)gdmN
EC number2.1.3.-
Keyword
Note
Note (GenBank)
  • putative carbamoyltransferase
Reference
ACC
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
Related Reference
PmId
[15355082] Inactivation of the carbamoyltransferase gene refines post-polyketide synthase modification steps in the biosynthesis of the antitumor agent geldanamycin. (J Am Chem Soc. , 2004)
[18600054] Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427. (J Microbiol Biotechnol. , 2008)
ACC
Q06BM1
NITE
Gelda_00040
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)
[18667782] A new post-PKS modification process in the carbamoyltransferase gene inactivation strain of Streptomyces hygroscopicus 17997. (J Antibiot (Tokyo). , 2008)
[20683449] 7-O-descarbamoyl-7-hydroxygeldanamycin, a minor component from the gdmN disruption mutant of Streptomyces hygroscopicus 17997. (J Antibiot (Tokyo). , 2010)
ACC
Q8KUG9
NITE
Ansam_00230
PmId
[14624546] The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. (J Am Chem Soc. , 2003)
[22195559] Dual carbamoylations on the polyketide and glycosyl moiety by asm21 result in extended ansamitocin biosynthesis. (Chem Biol. , 2011)

close this sectionSequence

selected fasta
>putative carbamoyltransferase [GdmN]
MLVLGLNGNFSAADTDVVPQLGEVFFHDSAASLIRDGELVAAVEEERLNRIKKTTKFPLN
AVRECLALAGARPEDVDAVGYYFPENHIDTVLNHLYTEYPRAPLRYSRELIRQRLKEGLG
WDLPDEKLVYVPHHEAHAYSSYLHSGMDSALVLVLDGRGELHSGTVYRAEGTRLEKLADY
PVPKSLGGLYLNATYLLGYGFGDEYKVMGLAPWGNPETYRDTFAKLYTLQDNGEYELHGN
IMVPNLVSPLFYAEGFRPRRKGEPFTQAHRDFAAALQETVEKIVLHILEYWAKTSGHSRL
CFGGGVAHNSSLNGLILKSGLFDEVFVHPASHDAGAGEGAAYAAAASLGTLERPGKRLLS
ASLGPALGGREQIRARLADWAPLIDVEFPDDAVETAAGLLAEGQVLGWAYGRSEFGPRAL
GHRSIVADARPEENRTRINAMVKKREGFRPFAPVVTAEAARDYFDLSGADGNHEFMSFVV
PVLPERRTELGAVTHVDGTARVQVVSAESGERFHRLVRRFGELTGTPVLLNTSFNNNAEP
IVQSLDDVVTSFLTTDLDVLVVEDCLVRGKASPDLGVLVPRFRPVTRLVERRTAGPDASA
GAKTHEIHLDYDGGPSAKVSPELYELLGAVDGTTTLGDLAKTVGGLSDALATEVFALWEQ
RFLTLAPAGDIGPLADDGTRGH
selected fasta
>putative carbamoyltransferase [GdmN]
GTGCTTGTGCTCGGGCTCAACGGCAACTTCTCCGCCGCGGACACCGATGTGGTGCCGCAG
CTCGGAGAGGTGTTCTTTCATGACTCGGCGGCTTCCTTGATCCGCGACGGCGAACTCGTG
GCCGCCGTGGAGGAGGAGCGGCTCAACCGGATCAAGAAGACAACCAAATTTCCCCTCAAC
GCGGTCCGTGAGTGCCTGGCCCTGGCCGGTGCGCGGCCCGAGGACGTCGACGCGGTGGGC
TACTACTTTCCCGAGAACCACATCGACACCGTCCTCAACCACCTCTACACCGAATATCCG
AGGGCGCCCCTGCGCTACTCCCGGGAGCTGATCCGGCAGCGGCTGAAGGAGGGCCTGGGC
TGGGACCTGCCGGACGAGAAGCTGGTGTACGTGCCGCACCACGAGGCGCACGCGTACTCC
TCGTATCTGCACTCCGGCATGGACTCCGCACTGGTCCTGGTGCTGGACGGCCGTGGCGAA
CTGCACTCCGGCACCGTCTACCGCGCCGAGGGCACGCGGCTGGAGAAGCTGGCCGACTAC
CCGGTGCCCAAGTCGCTCGGCGGGCTCTACCTGAACGCCACCTATCTGCTCGGCTACGGC
TTCGGCGACGAGTACAAGGTGATGGGTCTGGCCCCCTGGGGCAACCCGGAGACCTACCGC
GACACCTTCGCCAAGCTCTACACCCTCCAGGACAACGGCGAGTACGAGCTGCACGGCAAC
ATCATGGTGCCGAACCTGGTCAGCCCGCTGTTCTACGCCGAGGGCTTCCGGCCGCGCCGC
AAGGGCGAGCCGTTCACCCAAGCGCACCGCGACTTCGCCGCCGCGCTCCAGGAGACGGTC
GAGAAGATCGTGCTGCACATCCTCGAATACTGGGCGAAGACCAGCGGCCACTCCCGCCTG
TGCTTCGGCGGTGGCGTCGCCCACAACTCCAGCCTCAACGGGCTGATCCTCAAGTCCGGA
CTCTTCGACGAGGTGTTCGTGCACCCCGCCTCGCACGACGCGGGCGCGGGCGAGGGCGCC
GCCTACGCCGCGGCGGCGAGCCTTGGCACGCTGGAGCGCCCGGGGAAGCGGCTGCTCAGC
GCGAGCCTCGGCCCGGCACTGGGCGGCCGGGAGCAGATCAGGGCACGGTTGGCCGACTGG
GCGCCGCTGATCGATGTGGAGTTCCCGGACGACGCCGTGGAGACCGCGGCCGGACTCCTC
GCCGAGGGACAGGTGCTCGGCTGGGCGTACGGCCGCTCCGAGTTCGGCCCCCGCGCCCTG
GGCCACCGCAGCATCGTCGCGGACGCACGCCCCGAGGAGAACCGGACCCGCATCAACGCG
ATGGTGAAGAAGCGCGAGGGCTTCCGGCCGTTCGCCCCGGTGGTCACCGCCGAAGCCGCC
CGCGACTACTTCGACCTCTCCGGCGCGGATGGCAACCACGAGTTCATGTCCTTCGTGGTG
CCGGTGCTGCCGGAGCGGCGTACGGAACTCGGCGCGGTCACCCACGTGGACGGCACCGCC
CGGGTACAGGTCGTCTCCGCCGAGTCCGGCGAGCGGTTCCACCGCCTGGTGCGGCGATTC
GGCGAACTGACCGGCACCCCCGTGCTCCTCAACACCTCCTTCAACAACAACGCCGAACCC
ATCGTGCAGAGCCTCGACGACGTGGTCACCAGCTTCCTGACCACCGACCTGGACGTTCTG
GTGGTGGAGGACTGCCTGGTACGCGGCAAAGCCTCGCCCGACCTGGGCGTTCTGGTGCCG
CGGTTCCGCCCGGTGACCCGGCTGGTCGAGCGCAGGACGGCCGGTCCGGACGCCTCGGCG
GGAGCCAAGACCCACGAGATCCACCTCGACTACGACGGCGGCCCGTCCGCGAAGGTGTCG
CCCGAGCTGTACGAACTGCTCGGCGCGGTCGACGGCACCACCACCCTCGGGGATCTGGCC
AAGACCGTGGGCGGGCTGTCGGACGCACTGGCCACCGAGGTGTTCGCCCTGTGGGAGCAG
CGGTTCCTCACCCTGGCCCCGGCCGGGGACATAGGGCCGTTGGCCGACGACGGTACGCGG
GGGCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR003696 Carbamoyltransferase (Family)
 [139-503]  1.5e-87 PF02543
PF02543   CmcH_NodU
SignalP No significant hit
TMHMM No significant hit