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CDS information : Gelda2_00140


close this sectionLocation

Organism
StrainNRRL 3602 (=NBRC 14620)
Entry nameGeldanamycin
Contig
Start / Stop / Direction67,534 / 66,422 / - [in whole cluster]
56,234 / 55,122 / - [in contig]
Locationcomplement(66422..67534) [in whole cluster]
complement(55122..56234) [in contig]
TypeCDS
Length1,113 bp (370 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative glyceroyl-ACP biosynthesis protein
putative 1,3-bisphospho-D-glycerate phosphatase/glyceroyltransferase
Product (GenBank)GdmH
Gene
Gene (GenBank)gdmH
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • similar to FkbH (putative methoxymalonyl-ACP pathway protein)
Reference
ACC
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
Related Reference
ACC
Q1G727
NITE
Oxzol_00080
PmId
[16895402] The bifunctional glyceryl transferase/phosphatase OzmB belonging to the HAD superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis. (J Am Chem Soc. , 2006)
ACC
Q8KUG4
NITE
Ansam_00180
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
[17076505] On the biosynthetic origin of methoxymalonyl-acyl carrier protein, the substrate for incorporation of "glycolate" units into ansamitocin and soraphen A. (J Am Chem Soc. , 2006)
ACC
Q9KIE6
NITE
Asco_00080
PmId
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)
ACC
Q06BM0
NITE
Gelda_00050
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)

close this sectionSequence

selected fasta
>putative glyceroyl-ACP biosynthesis protein [GdmH]
MTEGKPVSEPPTAVKCLVWDLDNTLWRGTLLEDGEVLPFEWVRDVITTLDERGILQSIAS
KNDHDHAWERLEALGLAEYFVLPHIGWGPKSASLRAIADRLNFADRAMAFVDDQPAERAE
VTYRLPEVRCYAAEDLAGLTRLPEFSPAVVTVDSRQRRNMYQSGFRRDAERAEFSGPDED
FLRTLDIRMGISRATERELSRVEELTLRTSQMNATGVHYPDSALRGLLTDPAHEVLVITM
ADRFGPHGAVGIVLLERHPAVWHLKLLATSCRVVSYGAGSTLLSWLADQAAQAGVHLAAD
FRRTDRNRMMEVAYRFAGFADTTCACTVALAVPDEEGVERLHLTPACQPAPTTLRLTAPE
LADAALPRSR
selected fasta
>putative glyceroyl-ACP biosynthesis protein [GdmH]
ATGACGGAGGGGAAGCCCGTGAGCGAGCCGCCGACGGCCGTCAAATGTCTCGTCTGGGAC
CTGGACAACACACTGTGGCGCGGCACCCTGCTCGAAGACGGCGAGGTGCTGCCGTTCGAG
TGGGTGCGCGATGTCATCACCACCCTCGACGAACGCGGCATTCTCCAGTCGATCGCCAGC
AAGAACGACCACGACCACGCCTGGGAGCGCCTGGAAGCGCTGGGCCTGGCCGAGTACTTC
GTACTGCCGCACATCGGCTGGGGCCCCAAGTCCGCATCGCTGCGCGCCATCGCCGATCGG
CTGAACTTCGCCGACCGCGCCATGGCGTTCGTCGACGACCAGCCCGCCGAACGGGCCGAG
GTCACCTACCGGCTCCCCGAAGTGCGCTGCTACGCGGCCGAGGACCTGGCCGGGCTCACC
CGGCTGCCCGAGTTCAGCCCCGCCGTGGTCACCGTGGACTCGCGGCAGCGCCGGAACATG
TACCAGTCCGGGTTCCGCCGTGACGCCGAGCGGGCCGAGTTCAGCGGCCCCGACGAGGAC
TTTCTGCGCACCCTGGACATACGCATGGGCATCTCCCGCGCCACGGAGCGGGAGCTGTCC
CGGGTCGAGGAACTGACCCTGCGCACCAGCCAGATGAACGCCACCGGTGTGCATTACCCC
GACTCCGCACTGCGCGGACTGCTCACCGACCCCGCACACGAGGTGCTGGTCATCACGATG
GCCGACCGGTTCGGTCCGCACGGGGCCGTCGGCATCGTGCTGCTGGAACGGCACCCCGCG
GTGTGGCATCTGAAACTGCTGGCCACCTCGTGCCGCGTGGTCTCCTACGGCGCCGGGTCC
ACCCTGCTGAGCTGGCTGGCCGATCAGGCGGCGCAGGCCGGGGTGCACCTGGCCGCCGAC
TTCCGGCGCACCGATCGCAACCGGATGATGGAGGTCGCCTACCGCTTCGCCGGGTTCGCC
GACACCACCTGCGCCTGCACCGTCGCCCTCGCGGTCCCCGACGAGGAGGGGGTCGAACGC
CTCCACCTCACCCCCGCGTGCCAGCCGGCTCCGACGACGCTGCGGCTGACGGCGCCTGAG
CTCGCCGACGCGGCGCTGCCGCGCTCCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR010033 HAD-superfamily phosphatase, subfamily IIIC (Domain)
 [15-123]  1e-19 TIGR01681
TIGR01681   HAD-SF-IIIC
IPR010037 FkbH domain (Domain)
 [12-321]  6.49999999999992e-111 TIGR01686
TIGR01686   FkbH
IPR016181 Acyl-CoA N-acyltransferase (Domain)
 [188-296]  1.40000045763982e-06 SSF55729
SSF55729   Acyl_CoA_acyltransferase
 [189-321]  8.89999999999998e-07 G3DSA:3.40.630.30
G3DSA:3.40.630.30   Acyl_CoA_acyltransferase
IPR023214 HAD-like domain (Domain)
 [14-116]  5.40000000000001e-10 G3DSA:3.40.50.1000
G3DSA:3.40.50.1000   HAD-like_dom
 [11-115]  1.69999922284049e-15 SSF56784
SSF56784   HAD-like_dom
SignalP No significant hit
TMHMM No significant hit