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CDS information : Gelda2_00150


close this sectionLocation

Organism
StrainNRRL 3602 (=NBRC 14620)
Entry nameGeldanamycin
Contig
Start / Stop / Direction68,643 / 67,531 / - [in whole cluster]
57,343 / 56,231 / - [in contig]
Locationcomplement(67531..68643) [in whole cluster]
complement(56231..57343) [in contig]
TypeCDS
Length1,113 bp (370 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)GdmI
Gene
Gene (GenBank)gdmI
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • similar to FkbI; putative methoxymalonyl-ACP biosynthesis pathway protein; acyl-CoA dehydrogenase homolog
Reference
ACC
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
Related Reference
ACC
Q9KIE5
NITE
Asco_00090
PmId
[14623185] Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis. (J Mol Biol. , 2003)
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)
ACC
Q8KUG3
NITE
Ansam_00170
PmId
[11960423] Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis. (J Am Chem Soc. , 2002)
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q06BL9
NITE
Gelda_00060
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)

close this sectionSequence

selected fasta
>putative oxidoreductase [GdmI]
MTDAATSHTELVSGLIGDRADAWDLAGELPRDLLVKLGASGVLCAQVGAEHGGTGLDSHA
NGELTAAVGARCSSLRSVMTSQGMAAWTVRRLGGAEQWGTFLPRLTSGDLAAVGFSEPGA
GSDLAAMETEIADDGTHVVVTGRKVWITAAHYADLLVVFGKYRGGAAAVVVPAQAPGVRI
TRVPDPLGCRAAGHADITLDAVRVPAGHVLGGTGLPLPLATTAALTYGRMSVAWGCVGIL
RACLDAAATHTASREQSGRKLAEHQLVARHLAELYVAERHATRACEHASASWDTGSPDMA
VDAVHAKYVASREAAEGAARAVQLLASAAASDGHVVARAYRDAKLMEVIEGTSEICQLVL
AQHTRKKVQA
selected fasta
>putative oxidoreductase [GdmI]
GTGACCGACGCCGCCACCAGCCATACGGAGCTGGTCAGCGGGTTGATCGGGGACCGGGCG
GACGCCTGGGACCTGGCCGGGGAGCTGCCCCGCGACCTCCTGGTCAAACTCGGCGCCTCC
GGTGTGCTGTGCGCGCAGGTCGGCGCCGAGCACGGCGGCACCGGACTGGACAGCCATGCC
AACGGGGAGCTCACCGCGGCGGTCGGCGCCCGGTGCAGCTCGCTGCGCAGCGTGATGACG
TCCCAGGGCATGGCGGCATGGACCGTACGGCGGCTGGGCGGTGCGGAGCAGTGGGGCACC
TTTCTGCCCCGGCTGACCTCCGGTGACCTGGCGGCGGTCGGATTCAGCGAGCCCGGGGCG
GGCAGCGACCTGGCGGCGATGGAGACCGAGATCGCCGACGACGGCACACACGTGGTCGTC
ACCGGGCGCAAGGTGTGGATCACCGCCGCCCACTACGCCGACCTGCTGGTGGTGTTCGGG
AAGTACCGCGGCGGCGCCGCGGCCGTGGTCGTGCCCGCCCAGGCCCCCGGCGTCCGCATC
ACGCGGGTGCCGGACCCGCTGGGCTGCCGCGCCGCCGGCCACGCGGACATCACCCTGGAC
GCGGTCCGGGTACCCGCCGGCCACGTACTCGGTGGCACCGGACTGCCGCTGCCCCTGGCG
ACCACCGCGGCGCTCACCTACGGGCGCATGTCCGTGGCGTGGGGGTGCGTCGGCATCCTG
CGCGCCTGCCTGGACGCCGCCGCCACGCACACCGCCAGCCGGGAACAGTCCGGCCGCAAA
CTCGCCGAGCACCAGCTGGTGGCCCGGCACCTGGCCGAACTGTACGTCGCGGAGCGGCAC
GCCACCCGGGCCTGCGAACACGCCAGCGCCTCCTGGGACACCGGCTCGCCCGACATGGCG
GTCGACGCGGTGCACGCCAAGTACGTCGCGTCCCGCGAGGCCGCCGAGGGCGCGGCACGC
GCCGTACAGCTCCTGGCGTCGGCCGCGGCATCCGACGGCCATGTGGTGGCCCGGGCCTAC
CGCGACGCGAAGCTGATGGAAGTCATCGAGGGCACCAGCGAGATCTGCCAGCTCGTCCTC
GCCCAGCACACGCGGAAGAAGGTGCAAGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain)
 [222-363]  1.99999999999999e-29 PF00441
PF00441   Acyl-CoA_dh_1
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain)
 [112-161]  1.4e-14 PF02770
PF02770   Acyl-CoA_dh_M
 [112-203]  7e-28 G3DSA:2.40.110.10
G3DSA:2.40.110.10   Acyl_CoA_DH/ox_M
IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain)
 [18-109]  1.9e-11 PF02771
PF02771   Acyl-CoA_dh_N
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain)
 [213-368]  2.70000183580794e-30 SSF47203
SSF47203   AcylCoADH_C_like
 [204-366]  9.8e-36 G3DSA:1.20.140.10
G3DSA:1.20.140.10   AcylCoA_DH_1/2_C
IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain)
 [12-214]  6.39998633403535e-51 SSF56645
SSF56645   AcylCoA_dehyd_NM
IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain)
 [15-109]  9.40000000000003e-22 G3DSA:1.10.540.10
G3DSA:1.10.540.10   AcylCoA_DH/ox_N
SignalP No significant hit
TMHMM No significant hit