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CDS information : Gelda2_00170


close this sectionLocation

Organism
StrainNRRL 3602 (=NBRC 14620)
Entry nameGeldanamycin
Contig
Start / Stop / Direction69,778 / 68,912 / - [in whole cluster]
58,478 / 57,612 / - [in contig]
Locationcomplement(68912..69778) [in whole cluster]
complement(57612..58478) [in contig]
TypeCDS
Length867 bp (288 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)GdmK
Gene
Gene (GenBank)gdmK
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • similar to FkbK; putative methoxymalonyl-ACP biosynthesis pathway protein; 3-hydroxy-CoA dehydrogenase homolog
Reference
ACC
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
Related Reference
ACC
Q9KIE3
NITE
Asco_00110
PmId
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)
ACC
Q8KUG1
NITE
Ansam_00150
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
ACC
Q9ADL9
NITE
Soraph_00040
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
ACC
Q06BL7
NITE
Gelda_00080
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)

close this sectionSequence

selected fasta
>putative oxidoreductase [GdmK]
MSGDAEAPRLTVLGAGTMGLGITSLVVGHGIPVTVVEVDEAKAGRTRAAVTERLRMAQLM
GALPAGRPQGELTVTASLADGRNATAVVEAVTEDTPTKAKVLEAVAGLTGARVPLISNTS
SIPIDELAGHIADPARLVGTHFMNPPYLIPTVEVIRGPRTGEAVMTAVTDLLRALERKPV
VVGDGPGFVTSRVLHPMINDAIRVVQEGTATVEAVDVLMRDCLGHRTGPLRTADLIGLDN
LADSLRVLQLRTGDARCAPCELLLKKVRDGHLGRKSGRGFYAYGKELA
selected fasta
>putative oxidoreductase [GdmK]
ATGAGCGGAGATGCGGAAGCGCCGCGGCTCACGGTGCTGGGCGCAGGAACGATGGGACTG
GGAATCACCTCTCTCGTCGTCGGGCACGGAATCCCGGTGACGGTCGTCGAGGTGGACGAG
GCCAAGGCCGGCCGAACACGGGCCGCGGTCACCGAACGGCTCCGCATGGCGCAGCTCATG
GGAGCCCTGCCGGCCGGTCGCCCGCAGGGCGAGCTGACGGTCACCGCGTCCCTGGCCGAC
GGCCGGAACGCCACCGCCGTGGTGGAGGCCGTCACCGAGGACACCCCCACCAAGGCCAAG
GTGCTCGAAGCGGTCGCCGGGCTCACCGGAGCGCGGGTGCCGCTGATCTCGAACACCTCC
TCCATCCCGATCGACGAACTGGCCGGTCATATCGCCGATCCGGCACGGCTGGTCGGCACC
CACTTCATGAACCCGCCCTATCTGATCCCGACGGTGGAGGTGATCCGCGGCCCCCGTACC
GGGGAAGCGGTGATGACCGCCGTGACGGATCTGCTGCGTGCGCTGGAGCGCAAGCCGGTC
GTCGTCGGCGACGGGCCGGGGTTCGTCACCAGCCGGGTGCTGCACCCGATGATCAACGAC
GCGATCCGGGTGGTGCAGGAGGGCACGGCGACGGTGGAGGCCGTGGACGTACTCATGCGG
GACTGCCTGGGCCACCGCACCGGGCCGCTGCGCACCGCGGACCTGATCGGCCTGGACAAC
CTCGCCGACTCGCTGCGCGTGCTGCAGCTACGTACCGGCGACGCCCGGTGCGCGCCGTGC
GAACTGCTGCTGAAGAAGGTCCGCGACGGCCACCTGGGCCGCAAGTCGGGCCGCGGGTTC
TACGCATACGGGAAGGAACTGGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
 [187-283]  2.2e-29 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
 [10-184]  4.39999999999997e-47 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
 [187-284]  1.39999892049878e-28 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
 [189-283]  1.69999999999999e-27 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
 [9-188]  2.19999999999997e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
 [1-288]  3.39999972437719e-63 PIRSF000105
PIRSF000105   HCDH
SignalP No significant hit
TMHMM No significant hit