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CDS information : Gelda2_00210


close this sectionLocation

Organism
StrainNRRL 3602 (=NBRC 14620)
Entry nameGeldanamycin
Contig
Start / Stop / Direction76,433 / 75,240 / - [in whole cluster]
65,133 / 63,940 / - [in contig]
Locationcomplement(75240..76433) [in whole cluster]
complement(63940..65133) [in contig]
TypeCDS
Length1,194 bp (397 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)GdmP
Gene
Gene (GenBank)gdmP
EC number
Keyword
  • C-4,5 desaturation
Note
Note (GenBank)
  • P450 (PikC subfamily)
Reference
ACC
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
Related Reference
ACC
Q06BL3
NITE
Gelda_00120
PmId
[21979086] Novel 4,5-dihydro-thiazinogeldanamycin in a gdmP mutant strain of Streptomyces hygroscopicus 17997. (Biosci Biotechnol Biochem. , 2011)
[22849774] Identification of 4,5-dihydro-4-hydroxygeldanamycins as shunt products of geldanamycin biosynthesis. (J Nat Prod. , 2012)
[23656556] 19-[(1'S,4'R)-4'-Hydroxy-1'-methoxy-2'-oxopentyl]geldanamycin, a natural geldanamycin analogue from Streptomyces hygroscopicus 17997. (J Nat Prod. , 2013)
PmId
[18600054] Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427. (J Microbiol Biotechnol. , 2008)

close this sectionSequence

selected fasta
>putative cytochrome P450 [GdmP]
MDEIRDYPESRAAACPFSPPLGYEELRERSAVTRVRMWDGSTPFLVTGYHEARAALGDSR
FSADGTHKAMPRFVKFEVPAEVFNLGRMDDPEHARIRRMLTANFTIRRTEAMRPMIQGIV
DGLLDRLIAQGPPADLVADFAFPLPSQVIGVMLGVSDADFAEFQQASQGVMDFTASAEEM
GAALGVMVDYVARMCAAKRADPGDDLLSRLIVDQELTGGLTQQQVVATALVLLLAGHETT
ANMIALSTVLLLSHPEQLARLRADAGLMGNAVDELLRYITIVQEGTGRVATEDVEVGGVL
IPGGEGVIINLPSANRDPHFADAHELDLSRPNAREHVAFGFGVHQCLGQTLARVELQIAL
ETLLRRLPTLRLEVPFDDLAFLYESMNFGVARVPVAW
selected fasta
>putative cytochrome P450 [GdmP]
ATGGACGAGATACGCGACTACCCCGAATCGCGGGCTGCTGCATGCCCGTTCTCACCCCCG
CTGGGATACGAGGAGTTGCGCGAGCGGTCCGCCGTCACGCGGGTGCGGATGTGGGATGGC
AGCACCCCGTTTCTCGTCACCGGCTATCACGAGGCGCGGGCCGCGCTCGGCGACAGCAGG
TTCAGCGCCGACGGCACGCACAAGGCGATGCCGCGCTTCGTGAAGTTCGAGGTGCCGGCC
GAGGTGTTCAACCTCGGGAGGATGGACGATCCGGAGCACGCCCGGATCCGCCGCATGCTC
ACCGCGAACTTCACCATCCGGCGCACCGAGGCGATGCGGCCGATGATCCAGGGCATCGTG
GACGGCCTCCTGGACCGGCTGATCGCCCAGGGGCCGCCGGCCGACCTGGTGGCCGACTTC
GCCTTCCCCCTGCCGTCCCAGGTGATCGGTGTGATGCTGGGGGTCTCGGACGCCGACTTC
GCGGAGTTCCAGCAGGCGTCGCAGGGCGTCATGGACTTCACCGCGTCGGCCGAGGAGATG
GGCGCCGCGCTCGGCGTCATGGTGGACTACGTCGCCCGGATGTGCGCGGCCAAGCGCGCC
GACCCGGGAGACGATCTCCTCAGCCGGCTCATCGTCGACCAGGAGCTGACGGGCGGGCTC
ACCCAGCAGCAGGTGGTCGCCACCGCCCTGGTGCTGCTGCTGGCCGGGCACGAGACCACC
GCCAACATGATCGCCCTGTCCACCGTCCTGTTGCTGAGCCACCCCGAACAGCTCGCCCGG
CTGCGGGCGGACGCCGGGCTGATGGGCAACGCGGTGGACGAACTGCTCCGGTACATCACG
ATCGTCCAGGAAGGCACGGGACGGGTGGCCACCGAGGACGTCGAGGTCGGCGGGGTGCTC
ATCCCGGGCGGTGAAGGGGTGATCATCAATCTGCCCAGCGCCAACCGCGACCCCCACTTC
GCGGACGCCCACGAACTGGACCTGAGCCGGCCGAACGCCCGCGAACATGTCGCGTTCGGC
TTTGGAGTGCACCAGTGCCTGGGGCAGACCCTCGCCCGGGTCGAGCTCCAGATCGCCCTG
GAGACCCTGCTGCGCCGGCTGCCGACGCTCCGCCTGGAGGTCCCGTTCGACGACCTGGCG
TTTTTGTACGAGTCGATGAACTTCGGCGTCGCCCGTGTGCCCGTCGCCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
 [235-252]  2.19999900980708e-07 PR00385 [270-281]  2.19999900980708e-07 PR00385 [337-346]  2.19999900980708e-07 PR00385 [346-357]  2.19999900980708e-07 PR00385
PR00385   P450
 [14-397]  4.60000044330866e-93 SSF48264
SSF48264   Cytochrome_P450
 [19-397]  1.20000000000001e-100 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
 [204-367]  1.5e-26 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
 [88-99]  7.79998304125571e-49 PR00359 [135-151]  7.79998304125571e-49 PR00359 [152-167]  7.79998304125571e-49 PR00359 [189-211]  7.79998304125571e-49 PR00359 [270-281]  7.79998304125571e-49 PR00359 [288-315]  7.79998304125571e-49 PR00359 [337-346]  7.79998304125571e-49 PR00359 [346-357]  7.79998304125571e-49 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
 [339-348]  PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit