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CDS information : Gelda_00080


close this sectionLocation

Organism
Strain17997
Entry nameGeldanamycin
Contig
Start / Stop / Direction26,954 / 26,088 / - [in whole cluster]
17,554 / 16,688 / - [in contig]
Locationcomplement(26088..26954) [in whole cluster]
complement(16688..17554) [in contig]
TypeCDS
Length867 bp (288 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)GdmK
Gene
Gene (GenBank)gdmK
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • methoxymalonyl-ACP biosynthesis pathway protein; fkbK
Reference
ACC
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)
Related Reference
ACC
Q9KIE3
NITE
Asco_00110
PmId
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)
ACC
Q8KUG1
NITE
Ansam_00150
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
ACC
Q9ADL9
NITE
Soraph_00040
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
ACC
Q84G15
NITE
Gelda2_00170
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)

close this sectionSequence

selected fasta
>putative oxidoreductase [GdmK]
MSGHAEAHRLMVLGAGTMGLGITSLAVGHGIPVTVVEVDEAKAGRTRAAVTERLRMAQLM
RALPADRPPGELTVTASPADGREATAVVEAVTEDTPTKAKVLEAVAGLTGGRVPLISNTS
SIPIDELAGFIADPARLVGTHFMNPPYLIPTVEVIRGPRTGEAAMAAVTDLLRALERKPV
VVGDGPGFVTSRVLHPMINDAIRVVQEGTATAEAVDVLMRDCLGHRTGPLRTADLIGLDN
LADSLRVLHERTGDARCAPSELLLEKVRDGHLGRKSGRGFYAYGKELA
selected fasta
>putative oxidoreductase [GdmK]
ATGAGCGGACATGCGGAAGCGCACCGGCTCATGGTGCTGGGTGCGGGAACAATGGGACTG
GGGATCACCTCTCTCGCCGTGGGGCACGGAATCCCGGTGACGGTCGTCGAGGTGGACGAG
GCCAAGGCCGGCCGGACGCGGGCCGCGGTCACCGAACGGCTCCGCATGGCCCAGCTCATG
CGGGCCCTGCCCGCCGACCGGCCGCCCGGCGAGCTGACGGTCACCGCGTCCCCGGCCGAC
GGCCGGGAGGCCACCGCCGTGGTGGAGGCCGTCACCGAGGACACCCCCACCAAGGCCAAG
GTGCTCGAAGCGGTCGCCGGGCTCACCGGCGGGCGGGTGCCGCTGATCTCGAACACCTCC
TCCATCCCGATCGACGAACTGGCCGGTTTCATCGCCGATCCGGCACGGCTGGTCGGCACC
CACTTCATGAACCCGCCCTATCTGATCCCCACGGTGGAGGTGATCCGCGGCCCCCGTACC
GGGGAAGCGGCGATGGCCGCCGTGACGGATCTGCTGCGCGCGCTGGAGCGCAAGCCGGTC
GTCGTCGGCGACGGGCCGGGGTTCGTCACCAGCCGGGTGCTGCACCCGATGATCAACGAC
GCGATCCGTGTGGTGCAGGAGGGCACGGCGACGGCGGAGGCCGTGGACGTCCTCATGCGG
GACTGCCTGGGCCACCGCACCGGACCGCTGCGCACCGCGGACCTGATCGGCCTGGACAAC
CTCGCCGACTCGCTGCGCGTGCTGCATGAACGCACCGGCGACGCCCGGTGCGCACCGAGC
GAATTGCTGCTGGAGAAGGTCCGCGACGGCCATCTGGGCCGCAAGTCGGGCCGCGGGTTC
TACGCATACGGGAAGGAGCTGGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
 [187-283]  1.7e-29 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
 [11-184]  1.7e-44 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
 [187-284]  1.5e-29 SSF48179
SSF48179   6-phosphogluconate dehydrogenase C-terminal domain-like
IPR013328 Dehydrogenase, multihelical (Domain)
 [189-283]  1.5e-27 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   no description
IPR016040 NAD(P)-binding domain (Domain)
 [6-188]  8.8e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   no description
SignalP
 [1-26]  0.339 Signal
Bacteria, Gram-negative   
 [1-30]  0.791 Signal
Eukaryota   
TMHMM No significant hit