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CDS information : Grana_00280


close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction31,629 / 31,886 / + [in whole cluster]
31,629 / 31,886 / + [in contig]
Location31629..31886 [in whole cluster]
31629..31886 [in contig]
TypeCDS
Length258 bp (85 aa)
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close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein
Gene
Gene (GenBank)gra-orf3
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[2583128] Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tu22. (EMBO J. , 1989)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
[8468280] Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins. (J Bacteriol. , 1993)
[9305965] Utilization of enzymatically phosphopantetheinylated acyl carrier proteins and acetyl-acyl carrier proteins by the actinorhodin polyketide synthase. (Biochemistry. , 1997)
Related Reference
ACC
Q02054
NITE
Actino_00200
PmId
[16285746] Self-malonylation is an intrinsic property of a chemically synthesized type II polyketide synthase acyl carrier protein. (Biochemistry. , 2005)
[19361520] Probing the Interactions of early polyketide intermediates with the Actinorhodin ACP from S. coelicolor A3(2). (J Mol Biol. , 2009)
[19381365] Preliminary kinetic analysis of acyl carrier protein-ketoacylsynthase interactions in the actinorhodin minimal polyketide synthase. (Mol Biosyst. , 2009)

close this sectionPKS/NRPS Module

ACP5..78

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MARLTLDGLRTILVACAGEDDGVDLSGDILDITFEELGYDSLALMESASRIERELGVALA
DGDINEELTPRVLLDLVNGAQAEAA
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
ATGGCTCGGCTCACCCTCGACGGACTGCGCACCATCCTCGTCGCCTGCGCCGGAGAGGAC
GACGGCGTCGACCTCTCCGGCGACATCCTCGACATCACGTTCGAGGAGCTCGGCTACGAC
TCCCTCGCCCTCATGGAGAGCGCCTCCCGCATCGAGCGCGAGCTCGGAGTCGCCCTCGCC
GACGGTGACATCAACGAGGAGCTGACCCCCCGCGTCCTCCTCGACCTCGTCAACGGCGCC
CAGGCCGAGGCGGCCTGA
ACP5..78
ACP13..234

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
 [36-51]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [5-78]  PS50075
PS50075   ACP_DOMAIN
 [1-84]  1.20000117458134e-13 SSF47336
SSF47336   ACP_like
 [5-81]  1.6e-12 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [29-76]  3.6e-08 PF00550
PF00550   PP-binding
SignalP No significant hit
TMHMM No significant hit