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CDS information : Herb_00240


close this sectionLocation

Organism
StrainAM 3672
Entry nameHerbimycin A
Contig
Start / Stop / Direction76,137 / 75,271 / - [in whole cluster]
76,137 / 75,271 / - [in contig]
Locationcomplement(75271..76137) [in whole cluster]
complement(75271..76137) [in contig]
TypeCDS
Length867 bp (288 aa)
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close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)HbmK
Gene
Gene (GenBank)hbmK
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • similar to FkbK (methoxymalonyl-ACP biosynthesis pathway protein)
Reference
ACC
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
Related Reference
ACC
Q8KUG1
NITE
Ansam_00150
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
ACC
Q9ADL9
NITE
Soraph_00040
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
ACC
Q9KIE3
NITE
Asco_00110
PmId
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)

close this sectionSequence

selected fasta
>putative oxidoreductase [HbmK]
MSGDAEAPRLTVLGAGTMGLGITSLAVGHGIPVTVVEVDEAKAGRTRAAVTERLRMAQLM
GALPAGRPRGELTVTASLADGRNATAVVEAVTEDTPTKAKVLEAVAGLTGARVPLISNTS
SIPIDELAGHIADPARLVGTHFMNPPYLIPTVEVIRGPRTGEAVMTAVTDLLRALERKPV
VVGDGPGFVTSRVLHPMINDAIRVVQEGTATVEAVDVLMRDCLGHRTGPLRTADLIGLDN
LADSLRVLHERTGDSRCAPSELLLKKVRDGHLGRKSGRGFYAYGKELA
selected fasta
>putative oxidoreductase [HbmK]
ATGAGCGGAGATGCGGAAGCGCCGCGGCTCACGGTGCTGGGCGCAGGAACGATGGGACTG
GGAATCACCTCTCTCGCCGTCGGGCACGGAATCCCGGTGACGGTCGTCGAGGTGGACGAG
GCCAAGGCCGGCCGAACACGGGCCGCGGTCACCGAACGGCTCCGCATGGCGCAGCTCATG
GGAGCCCTGCCGGCCGGCCGCCCGCGGGGCGAGCTGACGGTCACCGCGTCCCTGGCCGAC
GGCCGGAACGCCACCGCGGTGGTGGAGGCCGTCACCGAGGACACCCCCACCAAGGCCAAG
GTGCTCGAAGCGGTCGCCGGGCTCACCGGAGCGCGGGTGCCGCTGATCTCGAACACCTCC
TCCATCCCGATCGACGAACTGGCCGGTCATATCGCCGATCCGGCACGGCTGGTCGGCACC
CACTTCATGAACCCGCCCTATCTGATCCCCACGGTGGAGGTGATCCGCGGTCCCCGGACC
GGGGAAGCGGTGATGACCGCCGTGACGGATCTGCTGCGTGCGCTGGAGCGCAAGCCGGTC
GTCGTCGGCGACGGGCCGGGGTTCGTCACCAGCCGGGTGCTGCACCCGATGATCAACGAC
GCGATCCGGGTGGTGCAGGAGGGCACGGCCACGGTGGAGGCCGTGGACGTACTCATGCGG
GACTGCCTGGGCCACCGCACCGGGCCGCTGCGCACCGCGGACCTGATCGGCCTGGACAAC
CTGGCCGACTCGCTGCGCGTGCTGCACGAGCGCACCGGCGACTCCCGGTGCGCGCCGAGC
GAACTGCTGCTGAAGAAGGTCCGCGACGGCCACCTGGGCCGCAAGTCGGGCCGCGGGTTC
TACGCATACGGGAAGGAACTGGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
 [187-283]  4.6e-30 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
 [10-184]  5.00000000000001e-47 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
 [187-284]  1.89999859865865e-29 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
 [189-283]  5.30000000000001e-29 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
 [9-188]  5.69999999999998e-42 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
 [1-288]  1.70000295590053e-64 PIRSF000105
PIRSF000105   HCDH
SignalP
 [1-26]  0.319 Signal
Bacteria, Gram-negative   
 [1-30]  0.553 Signal
Eukaryota   
TMHMM No significant hit