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CDS information : Kirro_00440


close this sectionLocation

Organism
StrainTü365
Entry nameKirromycin
Contig
Start / Stop / Direction100,994 / 102,361 / + [in whole cluster]
100,994 / 102,361 / + [in contig]
Location100994..102361 [in whole cluster]
100994..102361 [in contig]
TypeCDS
Length1,368 bp (455 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative crotonyl-CoA reductase
Product (GenBank)putative crotonyl-CoA reductase
Gene
Gene (GenBank)kirN
EC number1.3.1.-
Keyword
  • ethylmalonyl-CoA
Note
Note (GenBank)
Reference
ACC
PmId
[18291322] Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. (Chem Biol. , 2008)
Related Reference
ACC
Q53865
PmId
[8521864] Purification of crotonyl-CoA reductase from Streptomyces collinus and cloning, sequencing and expression of the corresponding gene in Escherichia coli. (Eur J Biochem. , 1995)
ACC
Q9RNU6
PmId
[10542184] Role of crotonyl coenzyme A reductase in determining the ratio of polyketides monensin A and monensin B produced by Streptomyces cinnamonensis. (J Bacteriol. , 1999)
ACC
B8XVS5
PmId
[17548827] Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. (Proc Natl Acad Sci U S A. , 2007)
[19458256] Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase. (Proc Natl Acad Sci U S A. , 2009)

close this sectionSequence

selected fasta
>putative crotonyl-CoA reductase [putative crotonyl-CoA reductase]
MQDIIDAVLDPDTRPEDFAALPVPESYRAVTLHADEVEMFVRVAAEDRDPRRSMHLDEVA
VPQVGPGEALVAVMAGSINYNTVWSALFEPLPTFTFLARYGRRSPLARRHDLPYHVIGSD
LSGVVLRTGPGVFAWNPGDEVVAHCLSAELEAPDGHSDTMLDPDQRIWGYETNFGGLAQL
ALVKANQLMPKPAHLTWEEAGTPGLVASTAYRQLVSRNGARLKQGDNVLIWGASGGVGAF
ATQFALAGGANPICVVSSPAKAAICRAMGADAIIDRADEGYTFWKDEYTQDPAEWKRFGR
RIRELTGGEDPDIVFEHPGRDTFGASVYVTRKGGTIVTCASTSGYRHEYDNRYLWMSLKR
IVGSHFANYREAWESNRLIAKGRIHPTLSRTYTLEHAGQAVDDVYRNRHLGKVGVLALAP
HEGLGVRDTAMRERHLDAINRFRQTNRTPTGKGNK
selected fasta
>putative crotonyl-CoA reductase [putative crotonyl-CoA reductase]
ATGCAAGACATCATCGACGCCGTTCTCGACCCGGACACCCGGCCGGAGGACTTCGCCGCG
CTGCCCGTCCCGGAGAGCTACCGCGCCGTGACCCTGCACGCGGACGAGGTCGAGATGTTC
GTCCGGGTCGCGGCCGAGGACCGCGATCCGCGCCGGTCGATGCACCTCGACGAAGTCGCC
GTGCCCCAGGTCGGGCCCGGCGAGGCGCTGGTGGCGGTGATGGCCGGCTCGATCAACTAC
AACACCGTGTGGTCCGCGCTCTTCGAACCGCTGCCGACGTTCACGTTCCTGGCCCGGTAC
GGCCGCCGCTCGCCCCTGGCCCGGCGGCACGACCTCCCCTACCACGTCATCGGCTCCGAC
CTCTCCGGAGTCGTGCTGCGTACCGGGCCCGGCGTGTTCGCGTGGAACCCCGGCGACGAG
GTCGTCGCGCACTGCCTGTCGGCCGAGTTGGAGGCGCCCGACGGGCACAGCGACACGATG
CTCGATCCGGACCAGCGCATCTGGGGGTACGAGACCAACTTCGGCGGCCTGGCACAACTG
GCGCTGGTCAAGGCGAACCAGCTGATGCCCAAACCGGCGCACCTGACCTGGGAGGAGGCC
GGCACACCCGGTCTGGTCGCCTCCACCGCGTACCGGCAACTGGTCTCCCGCAACGGTGCC
CGGCTGAAGCAGGGCGACAACGTGCTCATCTGGGGCGCGTCCGGCGGCGTGGGGGCGTTC
GCGACGCAGTTCGCGCTGGCCGGCGGCGCCAACCCGATCTGTGTGGTGTCCAGCCCGGCC
AAGGCCGCCATCTGCCGGGCGATGGGCGCCGACGCGATCATCGACCGTGCCGACGAGGGC
TACACCTTCTGGAAGGACGAGTACACCCAGGACCCGGCGGAATGGAAACGGTTCGGCCGC
CGGATCCGGGAGCTCACGGGTGGGGAGGATCCCGACATCGTCTTCGAGCATCCCGGCCGG
GACACCTTCGGCGCCTCGGTGTACGTCACCCGCAAGGGCGGCACCATCGTCACCTGTGCC
TCCACCTCCGGCTACCGCCACGAGTACGACAACCGCTACCTGTGGATGTCGCTGAAGCGG
ATCGTCGGATCGCATTTCGCCAACTACCGGGAGGCATGGGAGTCCAACCGGCTCATAGCC
AAGGGCAGGATCCACCCCACCCTCTCCCGCACGTACACCCTGGAACACGCCGGTCAGGCA
GTCGACGACGTGTACCGCAACCGGCACCTCGGCAAGGTCGGCGTGCTGGCCCTGGCACCG
CACGAGGGGCTGGGCGTCCGTGACACCGCGATGCGCGAGCGGCATCTCGACGCGATCAAC
CGCTTCCGGCAGACGAACCGCACGCCTACAGGGAAGGGAAACAAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR010085 Crotonyl-CoA reductase (Family)
 [32-428]  TIGR01751
TIGR01751   Crot-CoA-red
IPR011032 GroES-like (Domain)
 [39-233]  1.99999636034521e-25 SSF50129
SSF50129   GroES_like
IPR013149 Alcohol dehydrogenase, C-terminal (Domain)
 [236-380]  7.00000000000005e-38 PF00107
PF00107   ADH_zinc_N
IPR013154 Alcohol dehydrogenase GroES-like (Domain)
 [115-190]  5.5e-05 PF08240
PF08240   ADH_N
IPR016040 NAD(P)-binding domain (Domain)
 [231-364]  8.49999999999995e-24 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit