close all open/close all

CDS information : Lasal2_00110


close this sectionLocation

Organism
StrainATCC 31180
Entry nameLasalocid
Contig
Start / Stop / Direction12,046 / 10,490 / - [in whole cluster]
12,046 / 10,490 / - [in contig]
Locationcomplement(10490..12046) [in whole cluster]
complement(10490..12046) [in contig]
TypeCDS
Length1,557 bp (518 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative drug resistance transporter
Product (GenBank)putative resistance protein, transporter
Gene
Gene (GenBank)las2
EC number
Keyword
Note
Note (GenBank)
  • 77 permeases of the major facilitator superfamily
Reference
ACC
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
Related Reference
ACC
B6ZK58
NITE
Lasal_00070
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
ACC
O52557
NITE
Rifam_00370
PmId
[17351715] RifP; a membrane protein involved in rifamycin export in Amycolatopsis mediterranei. (Biotechnol Lett. , 2007)

close this sectionSequence

selected fasta
>putative drug resistance transporter [putative resistance protein, transporter]
MSVSDARRWWAVGALALSALVVGLDTTVLNVALPTLATDLEASTSELQWFANAYNLVFAA
VLLPAGLSGDRYGRKKLLVVALVLFGAASVGCAYSGSTDALIWWRALLGLGAGFIFPLSL
SVLPVMFSDEERPKAIGMVVTASALGLPLGPIVGGWLLDNFWWGSVFLINVPMVALAIVA
VLLLLPDSRSPEPPGLDLVGVLLSSAGLAGITYGAIKAGEKSWGDGTALTMLIGGVVLML
LFVVWQRHLAARPDGKPLVDLSLFRSPAFTWGAVMATVVSFALFGLLFTVPQYYQAVLGA
DALGTGLRLLPIIGGIIVGARVGGKVTAKAGPKRLVSVGFLLMAVGLFMGAVTDTDTSYG
YAATWIAVVGLGTGLALPLAVDSALGALSTERSGVGSAVIQTVRQVGGTIGVALLGTILT
SSYHSHLEVGSIPDRAADAAGESVSAGVLVAQKLHSPALLSSVQESFVHGMNVMLVVCGA
IAVVGTVLAALFMPQQAKREQPGTDPVAPADAQPRGAL
selected fasta
>putative drug resistance transporter [putative resistance protein, transporter]
ATGAGCGTCTCGGATGCCCGACGATGGTGGGCGGTGGGCGCCCTGGCGTTGAGTGCGCTG
GTGGTGGGCCTGGACACGACGGTCCTGAACGTCGCCCTGCCCACGCTCGCCACCGACCTG
GAGGCCTCGACCAGCGAACTGCAGTGGTTCGCCAACGCCTACAACCTGGTCTTCGCCGCG
GTGCTGCTGCCGGCGGGGCTCTCCGGTGACCGCTATGGCCGCAAGAAGCTGCTCGTGGTG
GCACTGGTCCTGTTCGGCGCCGCCTCCGTCGGCTGCGCCTACTCCGGCTCCACGGACGCC
CTGATCTGGTGGCGCGCCCTGCTCGGCCTGGGCGCCGGGTTCATCTTCCCGCTGTCCCTG
TCGGTGCTCCCCGTGATGTTCTCCGACGAGGAGCGACCGAAGGCGATCGGCATGGTCGTG
ACCGCCAGCGCGCTCGGGCTCCCGCTCGGCCCGATCGTCGGCGGCTGGCTGCTGGACAAC
TTCTGGTGGGGCTCGGTCTTCCTCATCAACGTCCCCATGGTCGCCCTCGCGATCGTCGCC
GTCCTGCTGCTGCTGCCCGACTCCCGCAGCCCGGAACCTCCGGGGCTCGACCTGGTGGGC
GTCCTGCTCTCCAGCGCCGGCCTCGCCGGCATCACTTACGGGGCCATCAAGGCCGGCGAG
AAGAGCTGGGGCGACGGCACGGCCCTCACGATGCTGATCGGCGGCGTGGTCCTGATGCTG
CTGTTCGTCGTGTGGCAGCGCCATCTCGCGGCGCGCCCCGACGGCAAGCCCCTGGTCGAC
CTCTCGCTCTTCAGGTCACCCGCGTTCACCTGGGGCGCGGTGATGGCCACAGTCGTGTCG
TTCGCCCTGTTCGGACTGCTGTTCACCGTGCCGCAGTACTACCAGGCCGTCCTCGGCGCC
GACGCCCTCGGCACGGGACTGCGGCTGCTGCCGATCATCGGCGGCATCATCGTCGGCGCC
CGGGTGGGCGGCAAGGTCACCGCCAAGGCGGGGCCCAAGCGGCTGGTCTCCGTGGGCTTC
CTGCTGATGGCCGTCGGACTGTTCATGGGAGCGGTGACCGACACCGACACCTCCTACGGC
TACGCGGCCACCTGGATCGCCGTCGTCGGCCTCGGCACGGGCCTCGCGCTCCCCCTGGCC
GTCGACTCGGCGCTCGGCGCGCTGTCGACCGAACGCAGCGGCGTCGGCTCCGCCGTGATC
CAGACGGTCCGCCAGGTCGGCGGCACCATCGGCGTGGCGCTCCTCGGCACCATCCTGACC
TCCTCCTACCACAGCCACCTGGAGGTGGGTTCGATCCCCGACCGGGCCGCGGACGCGGCA
GGGGAGAGCGTTTCCGCCGGCGTCCTCGTCGCGCAGAAGCTGCACTCGCCCGCCCTGCTC
TCCAGCGTGCAGGAGTCGTTCGTCCACGGCATGAACGTGATGCTGGTCGTCTGCGGCGCC
ATCGCCGTCGTCGGCACCGTGCTCGCGGCGCTGTTCATGCCGCAGCAGGCGAAGCGGGAG
CAGCCGGGCACCGACCCCGTCGCCCCGGCCGACGCCCAGCCGCGGGGCGCGCTCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR004638 Drug resistance transporter EmrB/QacA subfamily (Family)
 [13-490]  3.19999999999996e-80 TIGR00711
TIGR00711   Efflux_EmrB
IPR011701 Major facilitator superfamily (Family)
 [15-411]  7.00000000000005e-54 PF07690
PF07690   MFS_1
IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain)
 [1-502]  2.80000504261099e-80 SSF103473
SSF103473   MFS_gen_substrate_transporter
IPR020846 Major facilitator superfamily domain (Domain)
 [11-497]  PS50850
PS50850   MFS
SignalP No significant hit
TMHMM
 [12-34]  Transmembrane (i-o) [49-68]  Transmembrane (o-i) [77-96]  Transmembrane (i-o) [101-123]  Transmembrane (o-i) [136-158]  Transmembrane (i-o) [163-185]  Transmembrane (o-i) [198-216]  Transmembrane (i-o) [226-245]  Transmembrane (o-i) [268-290]  Transmembrane (i-o) [305-324]  Transmembrane (o-i) [336-353]  Transmembrane (i-o) [363-385]  Transmembrane (o-i) [406-423]  Transmembrane (i-o) [470-492]  Transmembrane (o-i)
Transmembrane 14