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CDS information : Lasal_00180


close this sectionLocation

Organism
StrainATCC 35851
Entry nameLasalocid
Contig
Start / Stop / Direction65,864 / 71,578 / + [in whole cluster]
65,864 / 71,578 / + [in contig]
Location65864..71578 [in whole cluster]
65864..71578 [in contig]
TypeCDS
Length5,715 bp (1,904 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)putative polyketide synthase
Gene
Gene (GenBank)lsd16
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
Related Reference
ACC
B5M9L3
NITE
Lasal2_00210
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
[22025396] Insights into lasalocid A ring formation by chemical chain termination in vivo. (Angew Chem Int Ed Engl. , 2011)

close this sectionPKS/NRPS Module

10 methylmalonyl-CoA
KS58..434
AT604..921
dh971..1135
KR1462..1642
ACP1747..1817

close this sectionSequence

selected fasta
>polyketide synthase [putative polyketide synthase]
MSNEPNEPQDSHEPNVRSAVREGALSNEERLRDYLKWVTTDLHDTRRRLREVQDAQSEPI
AIVGMACRFPGGADSPDTFWELIAEGRDAISGMPTDRGWDLDALYDDDPSHAGTSYAREG
GFLPDAGHFDAALFGISPREALAMDPQQRLVLEASWEALERAGINPQSLRGSRTGVFVGH
VASGYGAHVTDAPDGVEGYLGTGTSASVASGRVAYTLGLEGPAVTVDTACSSSLVALHWA
EQALRARECSLAIVAGVTVLPSPLAFIEFSRQRGLAADGRCKPFSAAADGFGFAEGVGAL
LVQRLTDARREGREVLAVIRGSAVNQDGASNGLTAPNGPSQQRVIRQALTNARLTPGDID
MVEAHGTGTALGDPIEAQALLATYGQDRPQDRPLRLGSVKSNIGHTQAAAGMAGLIKTVL
AMRHGVMPRTLHLEEPTPHVDWTSGRVSLLTEQTAWPASDQTAWPASDRPRRAGVSSFGM
SGTNAHVILEEAPQPSTADDADDAADGPARQEDGSAPVPWVLSGKSPEALRAQAARLRAH
VVASPAPVADLGLSLATTRAALEHRGVILATDLTDAVQGLDALASGEPAPGVVSGSAHPG
SGVVFVFPGQGSQWTGMGRELLDSSPEFAAYIAECDAALSDFVDWSLTDVLRGTEGAPGY
DRVDVVQPALFAVMVSLARLWQHHGIHPDAVIGHSQGEIAAAHIAGALTLNDAARIVALR
SQALLPLAGLGGMTSLALPHDQALNLIQPWGQDLSIASVNGPHSTVVSGTTHALDQLHTT
CGTQGVRARRIPVDYASHSAQVESIRDTVFQAATGINPQPTTIPLYSTVTGQPIDGTRLD
ADYWYTNLRHTVRFEETVRTLLSHGHRHFIETTAHPVLTLALEETAQATTTDPTITGTLR
RDHGDLTQLHTALATAWTHGLPVDWASLPAFRSARPVALPTYAFQRERYWLEEGHGGPAD
VEAAGLASPGHPLLGAMVRPAGGDPLVLTGRLSLRTHPWLADHVALGTVLLPGAAFVELA
LRAGDEVGCECLEELTLETPLVLPERGAVRTQVVIGEADGTGCRSLHVYSSPEDETDESG
PWTRHASGVLAKQADVTPFEFAVWPPADAEPVDLDGFYEGLAEVGHGYGPVFRGLRAAWR
LGPDVYAEVALPEEVGDEAGKFGLHPALLDASMHAIGVGDFFADTERVRLPFAWSDVSLH
AVGARTLRVRLSPADTDAVALQLADATGRQVASVGSMVSRPVSPEQLPAARQSERDLFSQ
VWEPVRASGAAPDPDTDWVVLGADTGPLVAAVSATGVRTAVHGDVVDLLAALDAGGAVPA
VALLFCDPVATGDVAADARACTSAVLSEVQTWLSDSRLESSQLVVVTRGAVTTGAEAEVT
DLVQAPVWGLLRSAQSENPARFTVVDLDDRPASHATLPGAVATALAVNEPQVALRDGVVV
VPRLARTDGPAQPVVPDWDPSGTVLITGGTGTLGALFARHLVTRYGVRHLLLTSRRGAEA
PGASELVAELAESGAAAEVVACDVADRGALAGVLAGIDPDHPLRAVVHTAGVLDDGVIGT
MTPERIDWVMRPKTDAAVHLHELTREQDLTAFVLFSSIAGLFGNPGQANYAAANVFTDAL
ARHRHAQGLPALSLAWGLWADATGMTGHLDEGERRRMSRSGVVPLSAEHGLALFDAACAL
DEPCVAPVQLDPPALRGLADAGVLPGVLRGLVRATARRAADHGGGDGGAVLVRRLAGLAE
ADRHRELLDLVRTQAANVLGHASSASIDPDHAFNELGFDSLTAVELRNLLNAATGLRLPP
SLVFDYPTPTRLAEHLRAGLEPEIGAVPHVDPEEAAVREALASLSLQQLREAGLVDVLLS
LAGGDDAMAGTGSEAEEETLSIATMDVDDLVQLALDTPETEFRK
selected fasta
>polyketide synthase [putative polyketide synthase]
ATGTCGAATGAGCCGAACGAGCCGCAGGACTCCCACGAGCCGAACGTGAGGTCGGCCGTG
CGCGAGGGTGCGCTCTCGAACGAGGAGAGGCTCCGCGACTACCTGAAGTGGGTCACCACC
GACCTGCACGACACGCGCCGCCGGCTGCGCGAGGTCCAGGACGCCCAGAGCGAACCGATC
GCGATCGTGGGGATGGCGTGCCGGTTCCCCGGCGGCGCCGACTCCCCGGATACCTTCTGG
GAGTTGATCGCCGAGGGCCGTGACGCGATATCGGGGATGCCCACCGATCGCGGCTGGGAC
CTGGACGCCCTCTACGACGACGACCCCAGCCACGCCGGGACGTCGTACGCACGCGAGGGC
GGCTTCCTGCCCGACGCCGGTCACTTCGACGCGGCCCTCTTCGGGATCTCACCCCGTGAG
GCCCTCGCGATGGACCCGCAGCAGCGGCTCGTCCTCGAGGCATCCTGGGAGGCACTGGAG
CGGGCCGGTATCAACCCCCAGTCGCTGCGCGGCAGTCGTACCGGCGTCTTCGTCGGCCAT
GTGGCCTCCGGATACGGCGCACACGTCACGGACGCTCCCGACGGGGTGGAGGGGTACCTG
GGTACCGGCACCTCCGCCAGTGTCGCCTCGGGCCGCGTCGCCTACACCCTGGGGCTGGAA
GGCCCGGCGGTCACCGTCGACACCGCCTGTTCCTCCTCGCTGGTGGCGCTGCACTGGGCC
GAGCAGGCCCTGCGGGCCCGCGAGTGCTCGCTGGCGATCGTCGCCGGGGTGACGGTGCTG
CCGTCCCCGCTGGCGTTCATCGAGTTCAGCCGGCAGCGCGGACTCGCCGCCGACGGCCGC
TGCAAGCCCTTCTCGGCGGCGGCCGACGGCTTCGGTTTCGCGGAGGGCGTGGGCGCCCTG
CTCGTGCAACGCCTGACGGACGCGCGCCGCGAGGGCCGCGAGGTCCTGGCGGTGATCCGC
GGCTCGGCCGTGAACCAGGACGGCGCCAGCAACGGCCTCACCGCACCCAACGGCCCCTCC
CAGCAGCGGGTGATCCGCCAGGCCCTCACCAACGCCCGCCTCACGCCCGGCGACATCGAC
ATGGTGGAGGCCCACGGCACCGGCACCGCGCTCGGCGACCCGATCGAGGCGCAGGCGTTG
CTGGCGACGTACGGCCAGGACCGCCCGCAGGACCGCCCGCTGCGGCTGGGGTCCGTGAAG
TCCAACATCGGGCACACCCAGGCGGCCGCGGGCATGGCCGGGCTCATCAAGACCGTCCTC
GCGATGCGGCACGGGGTGATGCCCCGCACCCTGCACCTCGAGGAACCCACCCCTCACGTC
GACTGGACCTCAGGCCGCGTCTCCCTGCTCACCGAGCAGACGGCCTGGCCCGCGTCGGAC
CAGACGGCCTGGCCCGCGTCGGACCGTCCGCGCCGGGCCGGTGTCTCCTCCTTCGGCATG
AGCGGCACCAACGCCCACGTGATTCTCGAGGAGGCCCCGCAGCCCTCCACCGCCGACGAC
GCCGACGACGCCGCTGACGGGCCCGCTCGCCAGGAGGACGGGTCGGCGCCCGTCCCCTGG
GTCCTGTCCGGCAAGAGTCCCGAGGCCCTGCGTGCCCAGGCCGCACGCCTGCGTGCGCAC
GTGGTGGCGTCCCCCGCTCCGGTCGCCGACCTCGGCCTGTCCCTGGCGACGACGCGCGCT
GCGCTGGAACACCGTGGGGTGATCCTGGCGACGGACCTCACCGACGCCGTCCAGGGGCTG
GACGCGCTGGCGTCAGGTGAGCCCGCGCCGGGAGTCGTGTCCGGCTCCGCACATCCGGGG
TCCGGGGTGGTGTTCGTGTTCCCGGGTCAGGGTTCGCAGTGGACCGGCATGGGACGCGAA
CTCCTCGACAGCTCGCCCGAGTTCGCCGCGTACATTGCCGAGTGCGATGCCGCGCTCAGC
GACTTCGTGGACTGGTCGCTCACCGACGTCCTGCGCGGCACCGAAGGAGCCCCCGGCTAC
GACCGCGTCGACGTCGTCCAGCCCGCCCTCTTCGCCGTCATGGTCAGCCTCGCCCGCCTC
TGGCAACACCACGGCATCCACCCCGACGCCGTCATCGGCCACTCCCAAGGCGAAATCGCC
GCCGCCCACATCGCCGGAGCCCTCACCCTCAACGACGCCGCCCGCATCGTCGCCCTACGC
TCCCAAGCCCTCCTCCCCCTCGCCGGCCTCGGCGGCATGACCTCCCTCGCCCTCCCCCAC
GACCAAGCCCTCAACCTCATCCAGCCCTGGGGCCAAGACCTGTCCATCGCCTCCGTCAAC
GGACCCCACTCCACCGTCGTCTCCGGCACCACCCACGCCCTCGACCAACTCCACACCACC
TGCGGCACCCAAGGAGTTCGGGCCCGCCGCATCCCCGTCGACTACGCCTCCCACTCCGCC
CAAGTCGAAAGCATCCGCGACACCGTCTTCCAAGCAGCCACCGGCATCAACCCACAGCCG
ACCACCATCCCCCTCTACTCGACGGTCACCGGCCAACCCATCGACGGCACACGCCTCGAC
GCCGACTACTGGTACACCAACCTCCGCCACACCGTCCGCTTCGAGGAGACGGTCCGCACC
CTCCTCAGCCACGGCCACCGCCACTTCATCGAGACCACCGCCCACCCCGTCCTCACCCTC
GCCCTCGAGGAGACGGCTCAGGCCACCACCACCGACCCGACCATCACCGGCACCCTCCGC
CGCGACCACGGCGACCTCACCCAACTCCACACCGCCCTCGCCACCGCCTGGACCCACGGT
CTCCCCGTCGACTGGGCGTCGCTGCCCGCGTTCCGATCCGCCCGGCCCGTGGCGCTGCCG
ACCTACGCGTTCCAGCGCGAACGGTACTGGCTGGAGGAGGGGCACGGCGGTCCCGCGGAC
GTCGAAGCGGCGGGACTCGCCTCTCCCGGACATCCGCTGCTCGGTGCCATGGTGCGACCC
GCCGGAGGTGACCCGTTGGTCCTCACCGGGCGGCTGTCCCTGCGCACGCACCCCTGGCTG
GCGGACCACGTGGCATTGGGCACCGTACTGCTGCCGGGTGCCGCTTTCGTGGAACTGGCG
TTGCGGGCGGGCGACGAGGTCGGGTGCGAGTGCCTGGAGGAACTCACCCTCGAGACGCCG
CTGGTCCTGCCGGAGCGCGGCGCGGTGCGCACGCAGGTGGTGATCGGCGAGGCCGACGGG
ACCGGGTGCCGGTCGCTGCACGTGTACTCCTCCCCCGAGGACGAGACCGATGAGTCAGGT
CCGTGGACGCGGCACGCGAGCGGTGTGCTGGCCAAGCAGGCCGACGTGACGCCGTTCGAG
TTCGCGGTGTGGCCGCCCGCGGACGCCGAACCGGTCGATCTGGACGGCTTCTACGAGGGT
CTGGCCGAGGTGGGACACGGCTACGGGCCGGTGTTCCGTGGGCTGCGCGCGGCCTGGCGG
CTGGGGCCGGACGTCTACGCGGAGGTGGCCCTGCCGGAAGAGGTCGGGGACGAGGCGGGG
AAGTTCGGGCTGCATCCGGCGCTGCTCGACGCCTCGATGCACGCCATCGGTGTAGGGGAC
TTCTTCGCCGACACCGAGCGGGTGCGGCTGCCGTTCGCATGGAGCGACGTGTCCCTCCAT
GCCGTGGGCGCCCGGACCTTGCGGGTGCGGCTGTCGCCGGCCGACACGGACGCGGTGGCA
CTGCAGTTGGCGGACGCGACAGGCCGCCAGGTCGCGTCCGTCGGGTCCATGGTGTCGCGT
CCTGTCTCGCCCGAGCAGCTGCCCGCCGCCCGACAGTCGGAGCGGGACTTGTTCAGCCAG
GTCTGGGAGCCGGTGCGGGCTTCGGGTGCCGCGCCGGATCCGGACACGGACTGGGTCGTC
CTCGGGGCGGACACGGGCCCCTTGGTCGCGGCCGTCTCGGCGACGGGCGTACGGACGGCG
GTCCACGGTGACGTCGTAGACCTGTTGGCGGCTCTGGACGCGGGTGGGGCCGTGCCGGCC
GTGGCCTTGCTGTTCTGCGATCCGGTGGCCACCGGTGACGTGGCCGCGGACGCCCGTGCC
TGCACGTCCGCGGTGTTGAGCGAGGTGCAGACCTGGCTGTCCGACTCCCGGCTGGAGTCG
TCGCAGTTGGTCGTGGTCACGCGAGGCGCGGTCACCACTGGTGCGGAGGCCGAGGTCACC
GACCTGGTGCAGGCTCCGGTGTGGGGGCTGCTGCGGTCGGCGCAGTCCGAGAACCCCGCA
CGCTTCACGGTGGTCGACCTGGACGACCGTCCTGCCTCTCACGCGACGCTGCCGGGCGCG
GTGGCCACCGCGCTCGCCGTGAACGAGCCGCAGGTCGCCCTGCGTGACGGGGTGGTGGTC
GTGCCGCGGCTCGCCCGGACGGACGGACCGGCGCAGCCTGTGGTCCCGGACTGGGACCCT
TCCGGCACCGTGCTGATCACCGGCGGTACGGGCACCCTCGGTGCCCTCTTCGCCCGGCAT
CTGGTAACGCGGTACGGCGTGCGGCACCTGCTGCTGACCAGCCGTCGCGGCGCGGAGGCT
CCCGGGGCGTCCGAACTGGTCGCGGAGCTGGCCGAGTCGGGGGCGGCGGCCGAGGTGGTC
GCGTGCGACGTGGCCGACCGTGGGGCGCTGGCCGGCGTGCTGGCGGGCATCGACCCCGAC
CACCCGCTGCGGGCGGTCGTGCACACGGCGGGTGTGCTGGACGACGGTGTGATCGGCACC
ATGACCCCGGAGCGGATCGACTGGGTCATGCGGCCCAAGACGGACGCCGCCGTCCACCTC
CACGAACTGACGCGGGAGCAGGACCTGACGGCGTTCGTGCTGTTCTCCTCGATCGCCGGG
CTGTTCGGCAACCCCGGTCAGGCCAACTACGCGGCAGCGAACGTGTTCACGGACGCGCTG
GCCCGGCACCGCCACGCACAGGGTCTGCCGGCGCTGTCCCTCGCCTGGGGTCTGTGGGCC
GACGCGACCGGCATGACAGGGCACCTCGACGAGGGCGAACGACGGCGGATGTCCCGAAGC
GGTGTGGTGCCGCTGTCGGCCGAGCACGGGCTGGCCCTCTTCGACGCGGCCTGCGCTCTG
GACGAGCCCTGTGTGGCCCCGGTCCAGCTCGACCCGCCGGCCCTGCGCGGTCTGGCCGAT
GCCGGGGTGCTGCCCGGCGTCCTGCGCGGCCTGGTCCGCGCCACGGCGCGGCGGGCCGCG
GACCATGGCGGCGGTGACGGCGGTGCGGTGCTGGTCCGGCGGCTCGCCGGTCTGGCCGAG
GCGGACCGCCACCGCGAACTCCTCGACCTCGTGCGCACGCAGGCGGCGAACGTCCTCGGC
CACGCCTCTTCCGCCTCCATCGACCCCGACCACGCCTTCAACGAGCTCGGCTTCGACTCG
CTCACGGCCGTCGAGCTGCGCAACCTGCTCAACGCCGCCACCGGGCTGCGGCTGCCGCCC
AGCCTCGTCTTCGACTACCCGACCCCGACCCGCCTCGCCGAGCACCTGCGCGCCGGCCTG
GAACCGGAGATCGGCGCCGTACCGCACGTCGACCCGGAGGAGGCCGCCGTGAGGGAGGCG
CTGGCCTCGCTGTCCTTGCAGCAGTTGCGCGAGGCCGGACTGGTCGACGTGCTGCTGAGC
CTCGCCGGGGGCGACGACGCCATGGCCGGCACCGGGAGCGAGGCGGAGGAGGAGACGCTG
TCGATCGCCACGATGGACGTCGACGATCTCGTGCAGCTCGCGCTCGACACCCCCGAGACG
GAGTTCCGCAAATGA
[10] KS58..434
[10] AT604..921
[10] methylmalonyl-CoA795..799
[10] dh971..1135
[10] KR1462..1642
[10] ACP1747..1817
[10] KS172..1302
[10] AT1810..2763
[10] methylmalonyl-CoA2383..2397
[10] dh2911..3405
[10] KR4384..4926
[10] ACP5239..5451

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [600-727]  5.69999999999998e-75 G3DSA:3.40.366.10 [795-912]  5.69999999999998e-75 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
 [1775-1790]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1745-1819]  1.1e-22 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1739-1834]  3.69999438558106e-23 SSF47336
SSF47336   ACP_like
 [1747-1817]  PS50075
PS50075   ACP_DOMAIN
 [1750-1816]  7.7e-14 PF00550
PF00550   PP-binding
IPR013968 Polyketide synthase, KR (Domain)
 [1462-1641]  1.40000000000001e-64 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [58-308]  2.59999999999995e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [316-434]  3.40000000000004e-50 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [604-921]  6.60000000000002e-103 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [26-51]  2e-13 PF08990
PF08990   Docking
 [26-58]  1.49999977583352e-06 SSF101173
SSF101173   Polyketide_synth_docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [602-891]  5.60004908290974e-67 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [729-794]  5.29999657550189e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [57-319]  1.10000000000001e-89 G3DSA:3.40.47.10 [320-495]  1.60000000000002e-67 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [50-432]  1.19999063421924e-103 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1462-1645]  4.6e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [221-237]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [606-903]  7.49999605851445e-124 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1748-1820]  2.69998997787809e-36 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
 [971-1135]  2.2999842048857e-87 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [60-494]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1462-1642]  4.39999001237724e-64 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit