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CDS information : Megalo_00030

Location

Organism	Micromonospora megalomicea subsp. nigra
Strain	NRRL 3275 (=NBRC 14744)
Entry name	Megalomicin
Contig	AF263245
Start / Stop / Direction	2,072 / 3,382 / + [in whole cluster] 2,072 / 3,382 / + [in contig]
Location	2072..3382 [in whole cluster] 2072..3382 [in contig]
Type	CDS
Length	1,311 bp (436 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-L-megosamine glycosyltransferase
Product (GenBank)	TDP-megosamine glycosyltransferase
Gene
Gene (GenBank)	megDI
EC number
Keyword	megosamine
Note
Note (GenBank)
Reference	ACC Q9F839 PmId [10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000) [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)

Sequence

>dTDP-L-megosamine glycosyltransferase [TDP-megosamine glycosyltransferase]
MRVVFSSMAVNSHLFGLVPLASAFQAAGHEVRVVASPALTDDVTGAGLTAVPVGDDVELV
EWHAHAGQDIVEYMRTLDWVDQSHTTMSWDDLLGMQTTFTPTFFALMSPDSLIDGMVEFC
RSWRPDWIVWEPLTFAAPIAARVTGTPHARMLWGPDVATRARQSFLRLLAHQEVEHREDP
LAEWFDWTLRRFGDDPHLSFDEELVLGQWTVDPIPEPLRIDTGVRTVGMRYVPYNGPSVV
PAWLLREPERRRVCLTLGGSSREHGIGQVSIGEMLDAIADIDAEFVATFDDQQLVGVGSV
PANVRTAGFVPMNVLLPTCAATVHHGGTGSWLTAAIHGVPQIILSDADTEVHAKQLQDLG
AGLSLPVAGMTAEHLRGAIERVLDEPAYRLGAERMRDGMRTDPSPAQVVGICQDLAADRA
ARGRQPRRTAEPHLPR

>dTDP-L-megosamine glycosyltransferase [TDP-megosamine glycosyltransferase]
ATGCGCGTCGTGTTTTCATCGATGGCTGTCAACAGCCATCTGTTCGGGCTGGTCCCGCTC
GCAAGCGCCTTCCAGGCGGCCGGACACGAGGTACGGGTCGTCGCCTCGCCGGCCCTGACC
GACGACGTCACCGGTGCCGGTCTGACCGCCGTGCCCGTCGGTGACGACGTGGAACTTGTG
GAGTGGCACGCCCACGCGGGCCAGGACATCGTCGAGTACATGCGGACCCTCGACTGGGTC
GACCAGAGCCACACCACCATGTCCTGGGACGACCTCCTGGGCATGCAGACCACCTTCACC
CCGACCTTCTTCGCCCTGATGAGCCCCGACTCGCTCATCGACGGGATGGTCGAGTTCTGC
CGCTCCTGGCGTCCCGACTGGATCGTCTGGGAGCCGCTGACCTTCGCCGCCCCGATCGCG
GCCCGGGTCACCGGAACCCCGCACGCCCGGATGCTGTGGGGTCCGGACGTCGCCACCCGG
GCCCGGCAGAGCTTCCTGCGACTGCTGGCCCACCAGGAGGTGGAGCACCGGGAGGATCCG
CTGGCCGAGTGGTTCGACTGGACGCTGCGGCGCTTCGGCGACGACCCGCACCTGAGCTTC
GACGAGGAACTGGTGCTGGGGCAGTGGACCGTGGACCCCATCCCCGAGCCGCTGCGGATC
GACACCGGCGTCCGGACGGTGGGCATGCGGTACGTCCCCTACAACGGCCCCTCGGTGGTG
CCCGCCTGGCTGTTGCGGGAACCCGAACGTCGGCGGGTCTGCCTGACCCTCGGCGGTTCC
AGCCGGGAACACGGCATCGGGCAGGTCTCCATCGGCGAGATGTTGGACGCCATCGCCGAC
ATCGACGCCGAGTTCGTGGCCACCTTCGACGACCAGCAGTTGGTCGGCGTGGGCAGCGTT
CCGGCAAACGTCCGTACCGCCGGGTTCGTGCCGATGAACGTCCTGCTGCCCACCTGCGCG
GCCACCGTGCACCACGGCGGCACCGGCAGTTGGCTGACCGCCGCCATCCACGGCGTACCG
CAGATCATCCTCTCGGACGCCGACACCGAGGTGCACGCCAAGCAGCTCCAGGACCTCGGC
GCGGGGCTGTCGCTCCCGGTCGCGGGGATGACCGCCGAGCACCTGCGTGGGGCGATCGAG
CGGGTTCTCGACGAGCCGGCGTACCGCCTCGGTGCGGAGCGGATGCGGGACGGGATGCGG
ACCGACCCGTCGCCGGCCCAGGTGGTCGGCATCTGTCAGGACCTGGCCGCCGACCGGGCG
GCACGCGGCAGGCAGCCGCGTCGAACCGCCGAGCCGCACCTGCCGCGATGA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro Database:interpro:26.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Protein of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-positive Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Megalo_00020 Megalo_00040

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