close all open/close all
Megalo_00030 Megalo_00030   Megalo_00050 Megalo_00050

CDS information : Megalo_00040


close this sectionLocation

Organism
StrainNRRL 3275 (=NBRC 14744)
Entry nameMegalomicin
Contig
Start / Stop / Direction3,462 / 4,634 / + [in whole cluster]
3,462 / 4,634 / + [in contig]
Location3462..4634 [in whole cluster]
3462..4634 [in contig]
TypeCDS
Length1,173 bp (390 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productmycarose O-acyltransferase
Product (GenBank)mycarose O-acyltransferase
Gene
Gene (GenBank)megY
EC number2.3.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000)
Related Reference
ACC
Q56074
PmId
[8074537] Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic. (Appl Environ Microbiol. , 1994)
ACC
Q00718
PmId
[1629172] A macrolide 3-O-acyltransferase gene from the midecamycin-producing species Streptomyces mycarofaciens. (J Bacteriol. , 1992)
ACC
P21542
PmId
[7764361] Cloning and nucleotide sequences of two genes involved in the 4''-O-acylation of macrolide antibiotics from Streptomyces thermotolerans. (Biosci Biotechnol Biochem. , 1993)

close this sectionSequence

selected fasta
>mycarose O-acyltransferase [mycarose O-acyltransferase]
MVTSTNLDTTARPALNSLTGMRFVAAFLVFFTHVLSRLIPNSYVYADGLDAFWQTTGRVG
VSFFFILSGFVLTWSARASDSVWSFWRRRVCKLFPNHLVTAFAAVVLFLVTGQAVSGEAL
IPNLLLIHAWFPALEISFGINPVSWSLACEAFFYLCFPLFLFWISGIRPERLWAWAAVVF
AAIWAVPVVADLLLPSSPPLIPGLEYSAIQDWFLYTFPATRSLEFILGIILARILITGRW
INVGLLPAVLLFPVFFVASLFLPGVYAISSSMMILPLVLIIASGATADLQQKRTFMRNRV
MVWLGDVSFALYMVHFLVIVYGADLLGFSQTEDAPLGLALFMIIPFLAVSLVLSWLLYRF
VELPVMRNWARPASARRKPATEPEQTPSRR
selected fasta
>mycarose O-acyltransferase [mycarose O-acyltransferase]
GTGGTTACCTCCACCAACTTGGACACGACAGCACGGCCGGCACTGAACTCGTTGACCGGG
ATGCGGTTCGTCGCCGCCTTCCTGGTCTTCTTCACGCACGTCCTGTCGAGGCTCATCCCG
AACAGCTACGTGTACGCCGACGGCCTGGACGCCTTCTGGCAGACCACCGGACGGGTGGGG
GTGTCGTTCTTCTTTATTCTCAGCGGTTTCGTGCTGACCTGGTCGGCGCGGGCCAGCGAC
TCGGTGTGGTCGTTCTGGCGCAGACGGGTCTGCAAGCTCTTCCCCAACCACCTGGTCACC
GCCTTCGCCGCCGTGGTGTTGTTCCTGGTCACCGGGCAGGCGGTGAGCGGTGAGGCGCTG
ATCCCGAACCTCCTGCTGATCCACGCCTGGTTCCCGGCCCTGGAGATCTCCTTCGGCATC
AACCCGGTGAGCTGGTCGTTGGCCTGCGAGGCGTTCTTCTACCTGTGCTTCCCGCTGTTC
CTGTTCTGGATCTCCGGTATCCGCCCGGAGCGGCTGTGGGCCTGGGCCGCCGTGGTGTTC
GCCGCGATCTGGGCGGTACCGGTGGTCGCCGACCTCCTGCTGCCGAGTTCCCCGCCGCTG
ATCCCGGGGCTTGAGTACTCCGCCATCCAGGACTGGTTCCTCTACACCTTCCCTGCGACG
CGGAGCCTGGAGTTCATCCTCGGGATCATCCTGGCCCGCATCCTGATCACCGGTCGGTGG
ATCAACGTCGGGCTGCTCCCCGCGGTGCTGTTGTTCCCGGTCTTCTTCGTCGCCTCGCTC
TTCCTGCCGGGTGTCTACGCCATCTCCTCGTCGATGATGATCCTTCCCCTGGTTCTGATC
ATCGCCAGCGGCGCGACGGCCGACCTCCAGCAGAAGCGCACCTTCATGCGTAACCGGGTG
ATGGTGTGGCTCGGCGACGTCTCCTTCGCGCTCTACATGGTCCACTTCCTGGTGATCGTC
TACGGGGCGGACCTGCTGGGGTTCAGCCAGACCGAGGACGCCCCGCTGGGTCTCGCACTC
TTCATGATCATTCCGTTCCTCGCGGTCTCCCTGGTGCTGTCGTGGCTGCTGTACAGGTTC
GTCGAGCTACCCGTCATGCGTAACTGGGCCCGCCCGGCCTCCGCCCGGCGCAAACCCGCC
ACGGAACCCGAACAGACCCCTTCCCGCCGGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2010_04 		show BLAST table
InterPro
Database:interpro:26.0
IPR002656 Acyltransferase 3 (Domain)
[16-358] 1.6e-29 PF01757
PF01757   Acyl_transf_3
SignalP
[1-46] 0.448 Signal
Bacteria, Gram-positive   
[1-36] 0.835 Signal
Eukaryota   
TMHMM
[13-35] Transmembrane (i-o) [50-72] Transmembrane (o-i) [93-115] Transmembrane (i-o) [143-165] Transmembrane (o-i) [172-194] Transmembrane (i-o) [214-236] Transmembrane (o-i) [243-261] Transmembrane (i-o) [265-287] Transmembrane (o-i) [299-321] Transmembrane (i-o) [336-358] Transmembrane (o-i)
Transmembrane 10   
Megalo_00030 Megalo_00030   Megalo_00050 Megalo_00050
Page top