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CDS information : Megalo_00110

Location

Organism	Micromonospora megalomicea subsp. nigra
Strain	NRRL 3275 (=NBRC 14744)
Entry name	Megalomicin
Contig	AF263245
Start / Stop / Direction	11,424 / 10,483 / - [in whole cluster] 11,424 / 10,483 / - [in contig]
Location	complement(10483..11424) [in whole cluster] complement(10483..11424) [in contig]
Type	CDS
Length	942 bp (313 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-4-keto-6-deoxyhexose C-4 ketoreductase
Product (GenBank)	TDP-4-keto-6-deoxyhexose 4-ketoreductase
Gene
Gene (GenBank)	megBIV
EC number
Keyword	mycarose
Note
Note (GenBank)
Reference	ACC Q9F831 PmId [10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000) [15870344] Production of the potent antibacterial polyketide erythromycin C in Escherichia coli. (Appl Environ Microbiol. , 2005) [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)

Sequence

>dTDP-4-keto-6-deoxyhexose C-4 ketoreductase [TDP-4-keto-6-deoxyhexose 4-ketoreductase]
MTRHVTLLGVSGFVGSALLREFTTHPLRLRAVARTGSRDQPPGSAGIEHLRVDLLEPGRV
AQVVADTDVVVHLVAYAAGGSTWRSAATVPEAERVNAGIMRDLVAALRARPGPAPVLLFA
STTQAANPAAPSRYAQHKIEAERILRQATEDGVVDGVILRLPAIYGHSGPSGQTGRGVVT
AMIRRALAGEPITMWHEGSVRRNLLHVEDVATAFTAALHNHEALVGDVWTPSADEARPLG
EIFETVAASVARQTGNPAVPVVSVPPPENAEANDFRSDDFDSTEFRTLTGWHPRVPLAEG
IDRTVAALISTKE

>dTDP-4-keto-6-deoxyhexose C-4 ketoreductase [TDP-4-keto-6-deoxyhexose 4-ketoreductase]
ATGACAAGACATGTCACACTTCTCGGCGTTTCTGGATTCGTCGGAAGCGCCCTTCTTCGC
GAATTTACCACACACCCTCTCCGACTGCGGGCCGTCGCGCGCACCGGGTCTCGCGACCAG
CCACCAGGCAGCGCCGGGATCGAGCACCTTCGAGTCGACCTGCTCGAACCCGGCCGGGTC
GCGCAGGTCGTCGCCGACACCGACGTCGTCGTGCATCTGGTCGCCTACGCCGCCGGCGGG
TCGACCTGGCGCAGCGCCGCCACGGTGCCCGAGGCCGAACGCGTCAACGCGGGCATCATG
CGCGACCTCGTCGCCGCGCTGCGGGCAAGACCCGGACCCGCGCCGGTGCTCCTGTTCGCC
AGCACCACCCAGGCCGCGAACCCCGCCGCCCCCAGCCGGTACGCCCAGCACAAGATCGAG
GCCGAGCGGATCCTGCGTCAGGCCACCGAGGACGGGGTCGTCGACGGGGTGATCCTGCGC
CTGCCCGCGATCTACGGCCACAGCGGCCCGTCGGGGCAGACCGGCCGGGGTGTCGTCACC
GCCATGATCCGGCGAGCCCTCGCCGGTGAGCCGATCACGATGTGGCACGAGGGCAGCGTG
CGCCGCAACCTCCTGCACGTCGAGGACGTGGCCACCGCGTTCACCGCCGCACTGCACAAC
CACGAGGCGCTGGTCGGCGACGTCTGGACGCCGAGCGCGGACGAGGCCCGACCCCTCGGC
GAGATCTTCGAGACCGTCGCCGCCAGCGTGGCACGTCAGACCGGCAACCCTGCGGTGCCC
GTCGTCAGCGTCCCTCCACCGGAGAACGCCGAGGCCAACGACTTCCGCAGCGACGACTTC
GACTCCACCGAGTTCCGGACGCTGACCGGCTGGCACCCCCGGGTGCCGCTGGCCGAGGGC
ATCGACCGGACCGTTGCCGCCCTCATCTCGACCAAGGAGTGA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro Database:interpro:26.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd SSF51735 NAD(P)-bd
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive Eukaryota
TMHMM	No significant hit

Megalo_00100 Megalo_00120

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