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CDS information : Megalo_00180

Location

Organism	Micromonospora megalomicea subsp. nigra
Strain	NRRL 3275 (=NBRC 14744)
Entry name	Megalomicin
Contig	AF263245
Start / Stop / Direction	47,403 / 46,660 / - [in whole cluster] 47,403 / 46,660 / - [in contig]
Location	complement(46660..47403) [in whole cluster] complement(46660..47403) [in contig]
Type	CDS
Length	744 bp (247 aa)

Annotation

Category	1.4 Other
Product	putative type II thioesterase
Product (GenBank)	TEII
Gene
Gene (GenBank)	megH
EC number
Keyword	type II thioesterase
Note
Note (GenBank)	putative thioesterase
Reference	ACC Q9F824 PmId [10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000)
Related Reference	ACC Q6W5Q5 NITE FR008_00110 PmId [18836004] Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008. (Appl Environ Microbiol. , 2008)

Sequence

>putative type II thioesterase [TEII]
MNTWLRRFGSADGHRARLYCFPHAGAAADSYLDLARALAPEVDVWAVQYPGRQDRRDERA
LGTAGEIADEVAAVLRDLVGEVPFALFGHSMGALVAYETARRLEARPGVRPLRLFVSGQT
APRVHERRTDLPDEDGLVEQMRRLGVSEAALADQGLLDMSLPVLRADHRVLRSYAWQAGP
PLRAGITTLCGDTDPLTTVEDAQRWLPYSVVPGRTRTFPGGHFYLADHVGEVAESVAPDL
LRLTPTG

>putative type II thioesterase [TEII]
ATGAACACCTGGTTGCGGCGGTTCGGGTCCGCCGACGGACACCGGGCCCGGCTGTACTGC
TTCCCGCACGCGGGAGCCGCCGCCGACTCGTACCTCGACCTCGCCCGGGCACTGGCCCCG
GAGGTCGACGTGTGGGCGGTGCAGTACCCGGGCCGGCAGGACCGACGTGACGAGCGGGCT
CTCGGCACCGCCGGGGAGATCGCCGACGAGGTCGCGGCCGTGCTGCGCGACCTCGTCGGC
GAGGTTCCGTTCGCCCTGTTCGGTCACAGCATGGGCGCCCTGGTCGCCTACGAGACCGCC
CGTCGCCTGGAGGCTCGTCCGGGGGTGCGACCGCTGCGGTTGTTCGTCTCCGGGCAGACC
GCCCCCCGGGTCCACGAGCGGCGCACCGACCTGCCCGACGAGGACGGACTCGTCGAGCAG
ATGCGCAGGCTCGGGGTCAGCGAGGCCGCATTGGCCGACCAGGGGCTGTTGGACATGTCG
CTGCCGGTGCTGCGGGCCGACCACCGGGTGCTGCGCTCCTACGCCTGGCAGGCCGGGCCG
CCGCTGCGGGCCGGCATCACCACCCTCTGCGGTGACACCGACCCGTTGACGACGGTGGAG
GACGCCCAACGCTGGCTGCCGTACTCGGTAGTCCCCGGCCGTACCCGGACCTTCCCGGGC
GGGCACTTCTACCTCGCCGACCACGTCGGGGAGGTGGCCGAGTCGGTGGCGCCGGACCTG
CTGCGGCTCACCCCCACCGGCTGA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro Database:interpro:26.0	IPR001031 Thioesterase (Domain) PF00975 Thioesterase
SignalP	No significant hit
TMHMM	No significant hit

Megalo_00170 Megalo_00190

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