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CDS information : Mith_00150


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Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction17,923 / 19,149 / + [in whole cluster]
17,923 / 19,149 / + [in contig]
Location17923..19149 [in whole cluster]
17923..19149 [in contig]
TypeCDS
Length1,227 bp (408 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)mtmK protein
Gene
Gene (GenBank)mtmK
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[8654997] Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin. (Gene. , 1996)
Related Reference
ACC
Q54496
NITE
Nogl_00380
PmId
[8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996)
ACC
Q54174
NITE
Urd_00130
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)

close this sectionPKS/NRPS Module

CLF7..364

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [mtmK protein]
MSADASQAVITGIGVAAPNGLSVKAWWDAVLRGESGIRRLSRFDPGRYPARLAGEIRDFV
DADHVPGRLLPQTDRVTRLSLAVAREAVEDAGVDLERLPRYAAGVSTASSAGGFEFGQRE
LQALWSKGGQYVSAYQSFAWFYAVNTGQISIGYDLRGPSGVLVTEQAGGLDAVAQARRLL
RRGSELMVTGGVDSALCPWGWTAHLAGGRLSTGDDPARAYLPFSADADGEVVGEGGALLV
LERAAAAPSRGARVYGVFAGYAATFDPPPGRGGTPQLARAAELALVDAGMTPDQVDVVFA
DAAGERAADRAEADALTRVFGARGVPVTAPKTMTGRLSAGGASLDLAAALLALRDQVVPP
TVNVTEPADDCPVDLVTGRPRPLPLRAALVLARGRGGFNAAAVVRALS
selected fasta
>polyketide synthase chain length factor subunit [mtmK protein]
ATGAGCGCCGACGCCTCGCAGGCGGTCATCACCGGCATCGGTGTCGCCGCGCCCAACGGG
CTGTCCGTCAAGGCCTGGTGGGACGCCGTGCTGAGGGGTGAGAGCGGGATCCGCAGGCTC
AGCCGGTTCGACCCCGGCCGCTATCCGGCACGGCTCGCCGGCGAGATCCGCGACTTCGTG
GACGCCGACCACGTTCCGGGCCGGCTGCTGCCCCAGACCGACCGGGTGACCAGGCTGTCC
CTCGCCGTGGCCCGGGAGGCCGTCGAGGACGCGGGCGTCGACCTGGAGCGGCTGCCCCGG
TACGCGGCCGGTGTCAGCACCGCGAGCTCGGCGGGCGGGTTCGAGTTCGGGCAGCGGGAG
CTGCAGGCCCTGTGGAGCAAAGGCGGGCAGTACGTCAGCGCGTACCAGTCGTTCGCCTGG
TTCTACGCCGTCAACACCGGGCAGATCTCGATCGGCTACGACCTGCGCGGTCCCAGCGGC
GTGCTGGTCACCGAGCAGGCCGGGGGGCTCGACGCGGTCGCCCAGGCCCGCCGCCTGCTG
CGCCGGGGCAGCGAGCTGATGGTCACCGGAGGCGTCGACTCCGCGCTGTGCCCCTGGGGC
TGGACCGCCCATCTGGCGGGCGGCCGGCTGTCCACCGGCGACGACCCGGCGCGTGCCTAC
CTGCCGTTCTCGGCGGACGCCGACGGGGAGGTCGTCGGTGAGGGAGGCGCCCTCCTGGTG
CTGGAGCGGGCCGCCGCGGCCCCGTCCCGCGGCGCCCGCGTCTACGGTGTCTTCGCCGGG
TACGCGGCCACCTTCGACCCGCCGCCCGGGCGGGGCGGCACCCCGCAGCTCGCCCGGGCG
GCCGAACTCGCCCTGGTCGACGCCGGGATGACCCCGGACCAGGTGGACGTCGTGTTCGCC
GACGCCGCGGGCGAGCGCGCCGCCGACCGAGCCGAGGCCGATGCCCTGACCCGGGTGTTC
GGCGCCCGTGGGGTGCCGGTCACCGCGCCGAAGACCATGACCGGGCGGCTCTCGGCCGGC
GGCGCCTCCCTGGACCTGGCGGCCGCCCTGCTGGCACTGCGCGACCAGGTCGTACCGCCC
ACCGTGAACGTCACCGAACCCGCCGACGACTGTCCCGTCGACCTGGTCACCGGCCGCCCC
CGGCCGCTGCCGCTGCGCGCCGCCCTCGTGCTGGCCAGGGGCCGCGGCGGATTCAATGCC
GCGGCCGTCGTGCGGGCCTTGTCCTGA
CLF7..364
CLF19..1092

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [7-245]  1.40000000000001e-53 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [255-364]  4.2e-26 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
 [6-259]  1.5e-55 G3DSA:3.40.47.10 [275-406]  1.09999999999999e-39 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [5-252]  1e-61 SSF53901 [215-407]  1.3000049540733e-48 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit