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CDS information : Mith_00310


close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction35,655 / 37,256 / + [in whole cluster]
35,655 / 37,256 / + [in contig]
Location35655..37256 [in whole cluster]
35655..37256 [in contig]
TypeCDS
Length1,602 bp (533 aa)
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close this sectionAnnotation

Category3.3 modification reduction
ProductBaeyer-Villiger monooxygenase
Product (GenBank)oxygenase
Gene
Gene (GenBank)mtmOIV
EC number
Keyword
  • ring opening
Note
Note (GenBank)
Reference
ACC
PmId
[9889148] Oxidative cleavage of premithramycin B is one of the last steps in the biosynthesis of the antitumor drug mithramycin. (Chem Biol. , 1999)
[11853433] Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway. (J Am Chem Soc. , 2002)
[12813091] Purification and characterization of a monooxygenase involved in the biosynthetic pathway of the antitumor drug mithramycin. (J Bacteriol. , 2003)
[16351075] Characterization of kinetics and products of the Baeyer-Villiger oxygenase MtmOIV, the key enzyme of the biosynthetic pathway toward the natural product anticancer drug mithramycin from Streptomyces argillaceus. (J Am Chem Soc. , 2005)
[16511225] Crystallization and X-ray diffraction properties of Baeyer-Villiger monooxygenase MtmOIV from the mithramycin biosynthetic pathway in Streptomyces argillaceus. (Acta Crystallogr Sect F Struct Biol Cryst Commun. , 2005)
[19364090] Crystal structure of Baeyer-Villiger monooxygenase MtmOIV, the key enzyme of the mithramycin biosynthetic pathway . (Biochemistry. , 2009)
Related Reference
ACC
Q70J79
NITE
Chro_00190
PmId
[21244022] Characterization of the terminal activation step catalyzed by oxygenase CmmOIV of the chromomycin biosynthetic pathway from Streptomyces griseus. (Biochemistry. , 2011)

close this sectionSequence

selected fasta
>Baeyer-Villiger monooxygenase [oxygenase]
MHNSNADDAALTTDVVVVGGGPVGLMLAGELRAGGVGALVLEKLVEPVGHDRAGALHIRT
VETLDLRGLLDRFLEGTQVAKGLPFAGIFTQGLDFGLVDTRHPYTGLVPQSRTEALLAEH
AREAGAEIPRGHEVTRLRQDAEAVEVTVAGPSGPYPVRARYGVGCDGGRSTVRRLAADRF
PGTEATVRALIGYVTTPEREVPRRWERTPDGILVLAFPPEGGLGPGWSSSSTGHSPAADE
GPVTLEDLGAAVARVRGTPLTLTEPVSWLSRFGDASRQAKRYRSGRVLLAGDAAHVHFPI
GGQGLNTGLQDAVNLGWKLAARVRGWGSEELLDTYHDERHPVAERVLLNTRAQLALMRPD
EQHTTPLRGFVEELLGTDEVNRYFTGMITGTDVRYATFAPAASARPHPWPGRFAGGLVLS
RPSGEPVPVAELLRSARPLLLDLAGRADLREATRPWSDRVSVVAGEATVEPPAQALLVRP
DGYVAWAGSPAATADELRASLARWFGPPANREPVGHQERAGRRGRPLSALKPE
selected fasta
>Baeyer-Villiger monooxygenase [oxygenase]
ATGCACAACAGCAACGCGGACGACGCCGCACTGACGACGGACGTCGTGGTGGTGGGAGGA
GGCCCGGTCGGCCTGATGCTGGCCGGGGAACTGCGGGCCGGCGGGGTGGGGGCCCTGGTG
CTCGAGAAGCTCGTCGAGCCCGTCGGCCACGACCGGGCGGGGGCCCTGCACATCCGGACG
GTGGAGACGCTGGACCTGCGCGGGCTGCTGGACCGGTTCCTGGAGGGAACGCAGGTCGCC
AAGGGGCTGCCCTTCGCCGGGATCTTCACCCAGGGCCTGGACTTCGGGCTGGTGGACACC
CGCCACCCGTACACGGGCCTGGTGCCGCAGTCGCGCACCGAGGCCCTGCTCGCCGAGCAC
GCGCGCGAGGCGGGCGCGGAGATCCCGCGCGGTCACGAGGTGACCCGGCTGCGCCAGGAC
GCGGAAGCGGTGGAGGTTACGGTGGCGGGGCCGAGCGGCCCGTACCCGGTGCGTGCCCGT
TACGGCGTCGGCTGCGACGGCGGGCGCAGCACGGTGCGGCGGCTGGCCGCGGATCGGTTT
CCCGGCACCGAGGCCACCGTCCGCGCCCTGATCGGCTACGTGACCACTCCCGAGCGCGAG
GTGCCGCGCCGCTGGGAGCGCACCCCGGACGGCATTCTGGTGCTGGCGTTCCCGCCGGAG
GGCGGCCTTGGGCCCGGGTGGTCGTCATCGAGTACGGGGCACTCCCCGGCGGCGGACGAG
GGACCGGTGACCCTGGAGGATCTCGGCGCCGCCGTCGCGCGGGTGCGGGGCACCCCCCTG
ACGCTGACCGAACCGGTGTCGTGGCTCTCCCGGTTCGGAGACGCGAGCCGCCAGGCGAAG
CGCTACCGCAGCGGACGGGTGCTGCTGGCCGGTGACGCGGCACACGTGCACTTCCCGATC
GGCGGCCAGGGGCTGAACACCGGTCTGCAGGACGCGGTCAACCTGGGCTGGAAGCTGGCG
GCCCGGGTCCGCGGGTGGGGTTCGGAGGAACTGCTCGACACCTACCACGACGAGCGGCAT
CCGGTGGCCGAGCGGGTCCTGCTCAACACACGGGCGCAGCTCGCCCTGATGCGCCCGGAC
GAGCAGCACACCACCCCGCTGCGCGGCTTCGTCGAGGAGTTGCTCGGCACGGACGAGGTG
AACCGGTACTTCACCGGAATGATCACCGGTACGGACGTGCGGTATGCGACGTTCGCCCCC
GCCGCTTCCGCGCGGCCGCATCCGTGGCCGGGCCGTTTCGCCGGCGGTCTGGTGCTGTCC
CGTCCGTCCGGTGAGCCGGTGCCGGTGGCCGAACTGCTGCGGTCGGCGCGGCCGCTGCTG
CTCGACCTCGCGGGGCGGGCCGATCTGCGGGAGGCGACGCGCCCGTGGTCCGACCGGGTC
TCCGTGGTCGCGGGCGAGGCGACCGTCGAGCCGCCCGCGCAGGCACTGCTGGTCCGCCCG
GACGGCTATGTCGCCTGGGCCGGTTCACCGGCCGCGACGGCGGACGAACTGCGCGCGAGC
CTGGCCCGCTGGTTCGGCCCGCCGGCCAACCGGGAGCCTGTCGGCCACCAGGAGCGCGCC
GGCCGCCGAGGGCGCCCCCTGAGCGCCTTGAAACCCGAATAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR002938 Monooxygenase, FAD-binding (Domain)
 [13-347]  2.99999999999998e-79 PF01494
PF01494   FAD_binding_3
IPR003042 Aromatic-ring hydroxylase-like (Domain)
 [14-36]  3.19999899046351e-45 PR00420 [158-173]  3.19999899046351e-45 PR00420 [284-299]  3.19999899046351e-45 PR00420 [299-315]  3.19999899046351e-45 PR00420 [317-335]  3.19999899046351e-45 PR00420 [335-351]  3.19999899046351e-45 PR00420
PR00420   RNGMNOXGNASE
SignalP No significant hit
TMHMM No significant hit