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CDS information : Myxa_00070


close this sectionLocation

Organism
StrainSga15
Entry nameMyxalamid
Contig
Start / Stop / Direction35,411 / 29,889 / - [in whole cluster]
35,411 / 29,889 / - [in contig]
Locationcomplement(29889..35411) [in whole cluster]
complement(29889..35411) [in contig]
TypeCDS
Length5,523 bp (1,840 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)MxaD
Gene
Gene (GenBank)mxaD
EC number
Keyword
Note
Note (GenBank)
  • PKS; contains KS, AT, DH, KR, ACP regions
Reference
ACC
PmId
[11182319] Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15. (Chem Biol. , 2001)

close this sectionPKS/NRPS Module

3 methylmalonyl-CoA
not conserved YASHS(Y->V)
KS20..396
AT550..866
DH911..1075
KR1442..1621
ACP1717..1790

close this sectionSequence

selected fasta
>polyketide synthase [MxaD]
MLLALDLHAESEALKRTRSEPIAIVGTSCRVPGGARTPEAFWRLLHGGVDAITEVPRDRW
DAEALFDPDPNKQGRTYARWGGFIDGVDRFDAAFFGVSPREASRMDPQQRMLLEVAWEAL
ERAGQPPDQLAGSRTGVFLGIIGSDYAQLQARLLGDSPDIYHLTGTSLNAAAGRLSYTLG
LQGPCMSIDTACSSSLVALHVACQSLRNRECDLALSAGVNLMLMPDATIALSSSRGLSPD
GRCKTFDAAANGFVRAEGCVVLVLKRLSDALASGDEILSLIAGSAVNQDGASSGLMVPNG
PAQERVIEQALASGGLKPSQISFVEAHGTGTSLGDPIELQALARVLGTGRSAETPLFVGS
VKTNVGHLEATAGLTGVLKTALALRNETIPPNLHFKRLNPDIVLDGAPVVVPTEPRSWPR
AEQPRFAGVSAFGISGTNAHIILQEPPVPAPQAVVPPRGSELLVLSARSPEALRAVAANH
ARWFSEHPDGALREVCATAALSRSHHDHRLALAGRTPAEFVEKLEAFSRGEAAPGAATGR
RTGQARRRVVFVFPGQGSQWVGMGRKLLEEEPSFRAALERCDAAIRACADFSVLGELAAE
AGRGRLHEIDVIQPVLFAMEVALAEMWRAWGIEPSAVVGHSMGEVAAAHVAGTLSLEDAA
KIICRRSRLLRSVSGQGSMLLVDLTMEEAKQALRGFEDRVSVAVSNSVRSTVLSGAPAAI
EEISNNLKQRDVFCRFVKVDVASHSPQMDPLRSELLAALQEIAPRAAHVPICSTVTGGMT
DGASFSANYWADNLREPVLFSKAIERLAVEGHDIFIELSPHPILLPAVEQHLRHLGREGA
VLPSLRRDEDERGSMLSSLGALYAMGHEVDLQRQHPVRGRRVALPTYPWQHERFWVEAPA
RSRRAQAAGHHPLLGAHVALAGQEGSHLWQTELFADSPSYLGDHVVHGEVVLPGTGYLEM
ALAGAAEALGSGKHALEDVIFQEMMVLPREEALSVQMSFVSDDDGAKRFRIFSRPADAGE
GWTLHAEGRIRQGVEAEAFALEEARARCTERMDGETHYQLMKERGVDFGPSFRLLKEMWK
GSGEAVARLELPSSVASEMAAHQLHPALLDACFQVINGAGLGERPGETFVPVAVENLRAY
GRPGRELWGHARVRSGTSSGPGSVEADVTLLDGEGRVLMEARGLMCRRLDAVRRRSADEI
DKWMYLVEWQEEPRGDVAPTPASAPGAWLVLADRQGFGKKLQAVLRERGEQCVLVSQGEE
SRLVEPGHYVVDLSKTEGFARILEAEFGSGRPACRGVVHLFSLDAPRPEEGLQALAKARS
LGAESALHLTQALVASGFRDMPRVWLVTEGVQAVKPAERVAHVEQAPIWGIGRVLSLEHP
EFRCCSVDLGALDEAQARVLADELFSSTLEEQVALRGTARFVARLSRMERSKAAPAELRA
DGTYLITGGLGGLGLKLAEWLVARGARHIALLGRGNGSAEAQQTLEALRAAGAEVRVCKA
DVAVRADLERVFGELAAGMPPLRGVFHAAAVLDDGVLVNLTSERLRAVMEPKIHGAWNLH
ELTASAPLDFFVLFAAAGSLLGSPGQGNYAAANVFLDALASYRRGLGLPALSVDWGSWAG
VGLAAAAEARGERIAQRGVDSMQPAQALEALGRVLESPHARVVVMRFELRQWREFYLTAA
QSPFLSRLAREQANSGESSANRGAFVETLRAAERPKRAQLLEAHLCEQAGHVLRMSPSRI
DPQQPLGSLGLDSLMGLEIRNRLEASLGLRLPATLVWRYPTVAALVEHLAEQLQLSITNK
AEQPQDEAAALEAEIVNNVKQLSDEEAEALLAEKLAALAE
selected fasta
>polyketide synthase [MxaD]
GTGCTGTTGGCGCTTGATCTGCACGCGGAGAGCGAGGCGCTCAAGCGCACGCGTTCAGAG
CCCATTGCCATCGTCGGTACCTCCTGCCGCGTGCCAGGTGGGGCGCGGACGCCGGAGGCG
TTCTGGCGCCTGCTGCACGGAGGCGTGGACGCCATCACCGAGGTGCCGCGTGATCGGTGG
GACGCGGAGGCGCTGTTCGATCCCGATCCCAACAAACAGGGCCGCACGTACGCGCGCTGG
GGTGGATTCATCGACGGCGTGGATCGCTTCGATGCCGCCTTCTTCGGGGTCTCGCCTCGC
GAGGCTTCCCGAATGGATCCCCAGCAGCGGATGCTGCTCGAGGTCGCGTGGGAGGCGCTG
GAGCGCGCAGGTCAGCCACCCGATCAGCTCGCCGGGAGTCGGACGGGCGTGTTCCTGGGG
ATCATTGGGAGCGACTACGCGCAACTCCAGGCGCGACTGCTCGGAGATTCCCCCGACATC
TACCATTTGACGGGCACGTCGCTGAACGCAGCCGCCGGTCGGCTTTCCTACACGCTCGGT
CTTCAGGGACCGTGCATGTCCATCGATACGGCTTGCTCGTCGTCGCTGGTAGCGCTCCAT
GTGGCGTGCCAGAGCCTGCGCAACCGCGAGTGCGATCTGGCGCTCTCCGCGGGCGTCAAC
CTCATGCTGATGCCGGATGCGACGATCGCGCTCTCGAGCAGCCGGGGGCTTTCGCCGGAT
GGGCGTTGCAAGACCTTCGATGCCGCAGCCAATGGCTTCGTGCGTGCCGAGGGGTGCGTC
GTCCTCGTGCTCAAGCGGCTCTCGGATGCGTTGGCCAGTGGTGACGAGATCCTCTCGCTC
ATCGCCGGTTCGGCGGTGAACCAGGATGGCGCCAGCAGTGGACTCATGGTGCCCAATGGC
CCCGCGCAAGAGCGGGTCATCGAGCAGGCGCTGGCCAGTGGTGGGCTCAAGCCGTCGCAG
ATCTCTTTCGTCGAGGCACACGGAACGGGAACCTCGCTGGGTGATCCCATCGAGTTGCAG
GCATTGGCGCGCGTGCTGGGGACGGGGAGGAGCGCGGAAACCCCGCTCTTCGTGGGCTCG
GTCAAGACGAACGTCGGACACCTGGAAGCGACGGCCGGGCTCACGGGCGTCCTGAAGACG
GCGCTTGCGCTGCGCAACGAGACGATTCCTCCGAACCTGCATTTCAAGCGCCTCAACCCG
GACATCGTCCTCGATGGCGCGCCCGTGGTCGTTCCGACCGAGCCGCGCTCGTGGCCGCGC
GCGGAGCAGCCGCGCTTCGCGGGCGTGAGCGCATTCGGCATCAGCGGAACCAACGCACAC
ATCATCCTGCAAGAGCCGCCTGTACCGGCTCCCCAAGCCGTGGTTCCCCCGCGAGGCTCG
GAGCTGCTCGTGCTTTCGGCGCGCAGTCCCGAAGCGCTTCGCGCGGTGGCCGCGAACCAT
GCGCGCTGGTTCTCGGAGCATCCGGACGGGGCGCTCCGCGAGGTGTGTGCCACGGCGGCC
TTGTCGCGTTCCCATCACGATCACCGCCTCGCGCTCGCGGGCCGCACGCCCGCCGAGTTC
GTGGAGAAGCTGGAGGCGTTCTCGCGTGGCGAGGCGGCCCCAGGGGCGGCCACCGGGCGC
AGAACAGGACAAGCCCGCCGTCGGGTGGTCTTCGTGTTCCCCGGCCAGGGCTCGCAGTGG
GTGGGCATGGGCCGGAAGTTGCTCGAGGAGGAGCCATCGTTCCGCGCCGCGCTGGAGCGG
TGTGACGCGGCCATTCGGGCGTGCGCGGACTTTAGTGTCCTCGGGGAGCTCGCGGCCGAG
GCGGGCCGTGGCCGTCTCCACGAGATCGACGTCATCCAGCCCGTGCTCTTCGCCATGGAA
GTGGCGTTGGCGGAGATGTGGCGCGCCTGGGGCATCGAGCCGTCCGCGGTGGTGGGGCAC
AGCATGGGCGAAGTGGCCGCGGCCCACGTGGCGGGCACGCTGAGCCTCGAGGATGCGGCG
AAGATCATCTGCCGCCGCAGTCGGTTGCTGCGGAGCGTGAGTGGCCAGGGCTCCATGCTC
CTGGTGGACCTGACGATGGAGGAGGCGAAGCAGGCCCTGCGAGGCTTCGAGGATCGCGTC
TCCGTCGCGGTGAGCAACAGCGTGCGGTCCACCGTTCTCTCGGGAGCGCCCGCTGCCATC
GAGGAGATCTCCAACAACCTCAAGCAGCGCGATGTCTTCTGCCGCTTCGTCAAGGTGGAC
GTCGCGTCGCACAGCCCGCAGATGGACCCGCTGCGGTCGGAGTTGCTCGCGGCCCTCCAG
GAAATCGCGCCCCGCGCAGCCCATGTCCCCATCTGCTCGACCGTGACGGGGGGGATGACG
GATGGCGCGTCGTTCAGTGCCAACTACTGGGCCGACAACCTGCGAGAGCCCGTGTTGTTC
TCGAAGGCGATCGAGCGGCTCGCGGTCGAAGGGCATGACATCTTCATCGAGCTGAGCCCG
CACCCCATCCTCCTGCCCGCCGTGGAACAACACCTGCGGCACCTCGGGCGTGAGGGCGCG
GTGCTGCCTTCGCTCAGGCGTGATGAGGACGAACGGGGCTCGATGCTGTCCTCATTGGGC
GCGCTGTACGCCATGGGGCACGAGGTAGACCTTCAGCGCCAGCACCCGGTGCGCGGTCGG
CGGGTGGCACTGCCCACCTACCCCTGGCAGCACGAGCGCTTCTGGGTCGAGGCGCCCGCG
CGTTCTCGTCGTGCTCAGGCAGCCGGGCATCACCCGCTGCTAGGAGCCCACGTGGCGCTG
GCGGGGCAGGAGGGGTCCCACCTGTGGCAGACGGAGCTCTTCGCGGACAGCCCCTCGTAC
TTGGGGGACCACGTTGTTCACGGTGAGGTCGTCCTTCCGGGGACTGGGTACCTTGAGATG
GCGCTCGCGGGGGCGGCGGAGGCGTTGGGGTCCGGGAAACACGCGCTCGAGGACGTGATC
TTCCAGGAGATGATGGTGCTGCCTCGCGAGGAGGCCCTCTCGGTGCAGATGAGCTTCGTT
TCGGACGATGACGGGGCGAAGCGTTTCCGGATCTTCAGCCGCCCGGCGGATGCAGGCGAG
GGCTGGACGCTGCACGCGGAGGGGCGGATCCGCCAGGGTGTCGAGGCAGAGGCCTTCGCG
CTCGAGGAGGCGCGTGCGCGGTGCACGGAGCGCATGGACGGGGAGACGCACTATCAGCTC
ATGAAGGAGCGAGGCGTTGACTTCGGCCCGAGCTTCCGCCTGCTGAAGGAGATGTGGAAG
GGTTCGGGAGAAGCGGTGGCCCGCCTGGAGTTGCCATCCTCGGTTGCTTCGGAGATGGCG
GCCCATCAGCTTCACCCAGCGCTGCTGGATGCCTGTTTCCAGGTCATCAATGGAGCGGGG
TTGGGCGAGCGGCCGGGCGAGACGTTCGTTCCGGTGGCGGTCGAGAACCTCCGTGCCTAC
GGGCGGCCGGGCCGTGAGCTATGGGGACACGCCCGCGTTCGGTCCGGTACGAGCAGTGGG
CCAGGCTCGGTCGAGGCGGATGTGACCTTGCTGGATGGGGAAGGTCGCGTCCTCATGGAG
GCCAGGGGCCTGATGTGCCGCCGCCTGGATGCGGTGCGGCGGCGCTCCGCGGACGAGATC
GACAAGTGGATGTACCTGGTCGAATGGCAGGAAGAGCCGCGAGGAGACGTTGCTCCCACC
CCGGCGTCCGCGCCTGGGGCGTGGCTCGTGCTCGCGGATCGCCAGGGTTTTGGCAAGAAG
TTGCAGGCGGTGCTGCGCGAGCGCGGCGAGCAGTGCGTCTTGGTTTCGCAGGGGGAGGAG
TCTCGCCTCGTCGAGCCCGGGCACTACGTGGTCGATCTATCGAAGACGGAAGGGTTCGCC
CGGATTCTCGAGGCCGAGTTCGGCTCCGGGCGACCTGCGTGCCGTGGGGTTGTGCATCTC
TTCAGCCTGGATGCGCCTCGGCCCGAAGAGGGGCTGCAGGCATTGGCGAAGGCGCGGTCG
CTCGGAGCGGAGAGTGCGCTCCACCTCACGCAGGCCCTCGTGGCTTCCGGCTTCCGGGAC
ATGCCCCGGGTGTGGCTGGTGACGGAAGGGGTCCAAGCCGTCAAACCCGCGGAGCGCGTG
GCCCACGTGGAGCAGGCCCCCATCTGGGGAATCGGACGGGTTCTCTCGTTGGAGCATCCC
GAGTTCCGTTGCTGCAGCGTGGACCTGGGGGCTCTGGACGAGGCTCAGGCGCGCGTGCTT
GCCGATGAACTCTTCTCGTCGACCCTGGAAGAGCAGGTCGCGCTTCGGGGGACAGCGCGG
TTCGTGGCGCGGTTGTCGCGCATGGAGCGCAGCAAGGCCGCACCGGCGGAGCTTCGCGCG
GATGGGACCTACCTGATTACCGGCGGTCTTGGTGGCTTGGGCCTGAAGCTCGCGGAGTGG
TTGGTCGCACGCGGCGCCCGCCACATCGCCTTGCTGGGCCGCGGAAACGGATCGGCAGAG
GCACAGCAGACCCTCGAAGCGTTGCGCGCGGCAGGCGCCGAGGTCCGAGTGTGCAAGGCC
GACGTGGCGGTGCGTGCGGACCTCGAACGGGTGTTCGGGGAACTCGCGGCTGGGATGCCT
CCGCTTCGGGGAGTCTTCCACGCGGCGGCGGTCCTCGATGACGGCGTGCTCGTGAACCTC
ACCTCCGAGCGCTTGCGCGCGGTGATGGAGCCCAAGATCCATGGCGCGTGGAACCTGCAT
GAGCTGACGGCGTCGGCACCGCTCGACTTCTTCGTGCTCTTCGCGGCGGCGGGTTCGCTC
CTGGGCTCTCCGGGACAGGGCAACTACGCGGCGGCCAACGTCTTCCTCGATGCGCTGGCG
TCGTACCGGCGGGGCCTCGGGTTGCCCGCGCTGAGCGTCGACTGGGGCTCTTGGGCGGGA
GTGGGTCTGGCGGCCGCGGCGGAGGCCCGCGGTGAGCGCATCGCGCAGCGGGGCGTGGAC
AGCATGCAGCCCGCGCAAGCCCTCGAGGCGTTGGGTCGAGTCCTGGAGAGCCCCCACGCG
CGTGTGGTGGTGATGCGCTTCGAGTTGCGGCAGTGGCGCGAGTTCTACCTCACGGCGGCG
CAGTCGCCGTTCCTGTCACGGCTTGCTCGCGAGCAGGCCAATAGCGGGGAAAGCTCGGCG
AACCGAGGGGCTTTCGTGGAAACGCTCCGGGCAGCGGAAAGGCCGAAGCGGGCGCAGTTG
CTGGAAGCCCACCTGTGTGAGCAGGCAGGCCACGTGCTCCGGATGTCTCCTTCGCGGATC
GATCCCCAGCAGCCGCTTGGCAGCCTGGGGTTGGACTCCCTGATGGGGTTGGAGATCCGC
AACCGACTCGAAGCCAGCCTGGGGCTGCGGCTCCCGGCGACGCTGGTGTGGCGCTACCCG
ACCGTGGCGGCGCTCGTCGAGCATCTGGCCGAGCAACTGCAACTTTCCATCACGAACAAG
GCCGAGCAGCCCCAGGATGAGGCGGCGGCGCTCGAAGCCGAAATCGTCAACAACGTCAAG
CAGCTCTCGGACGAAGAGGCCGAGGCGCTCCTCGCGGAGAAGCTCGCCGCGCTGGCTGAA
TAA
[3] KS20..396
[3] AT550..866
[3] methylmalonyl-CoA not conserved YASHS(Y->V)741..745
[3] DH911..1075
[3] KR1442..1621
[3] ACP1717..1790
[3] KS58..1188
[3] AT1648..2598
[3] methylmalonyl-CoA not conserved YASHS(Y->V)2221..2235
[3] DH2731..3225
[3] KR4324..4863
[3] ACP5149..5370

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [544-673]  2.20000000000002e-78 G3DSA:3.40.366.10 [741-858]  2.20000000000002e-78 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
 [1442-1607]  1.5e-57 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
 [1748-1763]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1717-1790]  PS50075
PS50075   ACP_DOMAIN
 [1725-1789]  5.40000000000001e-11 PF00550
PF00550   PP-binding
 [1720-1793]  1e-19 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1714-1793]  7.10001443313147e-21 SSF47336
SSF47336   ACP_like
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [20-270]  1.60000000000002e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [279-396]  2.99999999999998e-42 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [550-866]  2.50000000000001e-99 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [548-852]  8.89993011983204e-70 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [675-740]  2.59999946718995e-15 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [22-282]  1.10000000000001e-100 G3DSA:3.40.47.10 [283-447]  1.5e-62 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [12-394]  7.0999832511256e-105 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1441-1649]  4.39999999999997e-42 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [183-199]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [552-849]  9.40003071030835e-121 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1722-1793]  3.30000526807186e-30 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
 [911-1075]  2.1000026783403e-62 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [22-448]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1442-1621]  4.90001027782408e-60 SM00822
SM00822   PKS_KR
SignalP
 [1-19]  0.133 Signal
Eukaryota   
TMHMM No significant hit