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Myxo_00060 Myxo_00060   last last

CDS information : Myxo_00070


close this sectionLocation

Organism
StrainDW4/3-1
Entry nameMyxothiazol
Contig
Start / Stop / Direction37,285 / 42,537 / + [in whole cluster]
37,285 / 42,537 / + [in contig]
Location37285..42537 [in whole cluster]
37285..42537 [in contig]
TypeCDS
Length5,253 bp (1,750 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)MtaG
Gene
Gene (GenBank)mtaG
EC number
Keyword
  • glycine
Note
Note (GenBank)
  • NRPS; peptide synthetase domains: Condensation, adenylation, PCP, TE note: motif with homology to monooxygenases located between adenylation core regions A4 and A5; this motif may be involved in the formation of the terminal amide structure
Reference
ACC
PmId
[10601310] New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1. (J Biol Chem. , 1999)
[14583260] Melithiazol biosynthesis: further insights into myxobacterial PKS/NRPS systems and evidence for a new subclass of methyl transferases. (Chem Biol. , 2003)
[16807964] A unique mechanism for methyl ester formation via an amide intermediate found in myxobacteria. (Chembiochem. , 2006)

close this sectionPKS/NRPS Module

8
C55..351
A548..739
Ox760..1069
A1098..1308
PCP1394..1461
TE1483..1729

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [MtaG]
MAELSKQDVTLWVEGERLKYRAPKGALSPETLSLLSTHKAALLPYLRRLAAEGESVHPLS
HGQQALWFVHQLAPDSSAYNTALSIRVVSELDIPALRRACQALIDRHGALRTTVATHQGQ
PIQKVRQRAEVHFEQVDVPGMELDALKQLVVRTYQAPFDLERGPLIRVHLFSRGPKDHVL
LLSIHHIVYDGWSLIVIGDELLRHLYATEKAGVPSGLAPPPVTYVDYVRWQSEMLAGPAG
ERLWDYWSKQLAGSLPVLNLPFARPRPAVQGYSGASVPVSLSAALTRQLKALSEREGSTL
YTTVLAAFMVLMHRYTGEEDILLGSPTLGRTQPQFARVVGNFMNMIPLRGNLSGNPVFRD
FLAQLRQTVVGGLAHQDYPFHLLVERLNPERHSNSSPIFQSVFMLQPAQQGNIYAGDEAN
PAMPGGLVLRPFEIPQQEGQFDLILELTETDSGLSGMLKYSTDLFDATGAQQILGHLETL
LESVVEGPGKRLSDLTLLKPSERHQVLQAWNETAGAFSRITCIHETFEAQAQKAPDAIAL
VSGDNRLTYRELDERGNRLAHRLRALGVGPEVRVGLCVHRGLDMVIGMIGILKAGGAYVP
MDPTYPADRLAFMLSDSQAPVVLTEERLRQRLPDTGAKVVCLDAPEEGFSGALGTPRSGV
HAGNVAYTLYTSGSTGRPKGVMVCHRNAESFFAAMDAPLDSQTPGTWLATTSMSFDISIL
EILFSLTRGFQVVIRGEQGAGLPVSAGHRKAPQFSLFYFASDERESTHGKYQLLLEGARF
ADQHGFTAVWTPERHFHPFGGIFPNPSVVSAALAATTRNIRIRAGSVVLPLHSPIRVAEE
WSIVDNLSNGRVDLSFASGWHPNDFVLAPERFADARSGLAGQIQTFKKLWRGEKVNFRNG
VGTDVAVQALPRPIQPDVAVWLTAAGNPETFRLAGELGTNILTHLLGQNLTELEKKIQIY
RDAWKAAGHGPGEGHVTLMLHTFVGDDAAEVRQKVQGPLRQYLKSSVGLLKTVIGPLAHG
AEFESLSEADIDVLLSRAIERYQDQMGLFGTPESCLPMVAKLRDLGVDEIACLIDFGVDR
ESTLAGLHHLNELRERSTQHGEPEDIPALVARHGVTHFQCTPSMLRMLLLEPGGTEALRP
LKKLLIGGEAFPAALGQQVRPLVAGEVLNMYGPTETTIWSSFHRLRPDEATLPIGRPILN
TQMYLLDRHLQPVPMGVPGELLIGGEGVARGYLDRPELTATRFIPDPFGAEPGARLYRTG
DLARYLPDGRIEFLGRMDQQVKVRGVRIEPGEIESALRLHPEIRQAAVVARADAAGEVSL
AAYVVAGPDAQVAPSELRRFLKDKLPVSMIPDHFIRLDALPLTPNKKLDVRALPAPDAPP
VELSVAYVAPRDALELELVALWEELFDLRPIGVASSFFQLGGHSLLAVRLMSRLRAKFGQ
QLPVSLLFQADTIQKLASVLRQEGLAAARTPLVRIQETGDQPPLFFMHPTGGDVLCYAPL
ARQLGPRQPFYALQAVVDQEAESIEAMAARYLEEVRKVRPKGPYRLGGWSTGGILAQAMA
RQLEEAGEQVELLMLLETWSPTVYQRSEGTTALMAWFATDLLGGAGAAQLEASKLETLDE
AAQLDYLFERASASGALPGVERSEMEQRFRIFAKTARALSRYQSDAYRGKVLFLQAEEAA
TPTAGTLPEPQASWGQSLEQVQMYRMPGNHYTMLQSPHVRAVADRMARALEQLSASAEPP
PGAVRAASAR
selected fasta
>non-ribosomal peptide synthetase [MtaG]
ATGGCAGAGCTGTCCAAGCAGGACGTCACGCTCTGGGTGGAAGGCGAACGGTTGAAGTAC
AGGGCTCCGAAGGGAGCCCTGTCTCCGGAGACCCTGAGCCTGCTCTCCACCCACAAGGCG
GCGCTCCTGCCGTACCTCCGCCGGCTCGCGGCGGAGGGGGAGTCCGTTCATCCGCTCTCT
CATGGGCAACAGGCGCTCTGGTTCGTGCACCAACTGGCGCCGGACAGCTCCGCGTACAAC
ACGGCGCTGTCCATTCGCGTCGTCTCCGAGTTGGACATCCCCGCGCTGCGGCGTGCCTGC
CAGGCGCTCATCGATCGCCATGGCGCGCTGCGCACCACGGTGGCGACCCACCAAGGGCAG
CCGATCCAGAAGGTCCGTCAGCGGGCCGAAGTCCACTTCGAGCAGGTGGACGTCCCGGGG
ATGGAGCTCGATGCACTGAAGCAACTGGTGGTGCGGACCTACCAGGCGCCGTTCGATCTG
GAGCGGGGTCCTCTGATCCGCGTGCACCTGTTCTCGCGCGGTCCCAAGGACCACGTGCTG
CTGCTCTCCATCCACCACATCGTCTATGACGGATGGTCGCTGATTGTCATTGGCGACGAA
CTTCTGCGGCACCTCTATGCCACGGAGAAGGCGGGGGTTCCCTCGGGTCTTGCGCCCCCG
CCGGTGACGTACGTGGACTATGTCCGCTGGCAGTCCGAGATGCTCGCGGGCCCCGCGGGA
GAGCGGTTGTGGGATTACTGGTCCAAGCAACTGGCAGGGTCCCTGCCGGTGCTGAACCTG
CCCTTCGCGCGTCCACGGCCCGCGGTGCAGGGGTACTCGGGCGCCTCGGTTCCTGTCTCG
CTGAGCGCGGCGCTGACCCGGCAGCTGAAGGCGCTCTCCGAGCGCGAGGGCTCCACGCTG
TACACCACGGTCCTCGCGGCGTTCATGGTGCTCATGCACCGCTACACGGGTGAGGAGGAC
ATCCTTCTCGGTTCGCCGACGCTCGGACGGACGCAGCCTCAGTTCGCGCGGGTCGTGGGC
AATTTCATGAACATGATTCCCTTGCGGGGGAATCTGTCTGGCAACCCGGTGTTTCGTGAC
TTCCTCGCGCAGCTTCGGCAGACGGTGGTCGGCGGACTCGCCCACCAGGATTATCCGTTC
CACCTCCTCGTGGAGCGGCTGAACCCGGAGCGTCACTCCAACAGCTCCCCCATCTTCCAG
TCCGTCTTCATGCTGCAGCCCGCGCAGCAGGGGAACATCTACGCCGGAGATGAGGCCAAC
CCCGCGATGCCGGGGGGGCTCGTGCTGAGGCCCTTCGAGATTCCGCAGCAGGAGGGGCAG
TTCGACCTGATCCTGGAGCTGACGGAGACGGACTCGGGCTTGAGCGGCATGCTCAAGTAC
AGCACGGACCTGTTCGACGCCACGGGGGCCCAGCAGATCCTGGGGCACCTGGAGACCCTG
CTGGAGAGCGTCGTCGAAGGGCCCGGCAAGCGCCTGTCCGACCTGACGCTCTTGAAGCCC
TCCGAGCGGCATCAGGTGCTCCAGGCGTGGAATGAGACGGCGGGAGCGTTCTCTCGGATC
ACGTGCATTCACGAGACGTTCGAGGCGCAGGCCCAGAAGGCTCCGGATGCCATTGCCCTG
GTGTCTGGGGACAATCGGCTGACCTACCGCGAGCTGGATGAACGCGGAAACCGTCTGGCC
CACCGCCTTCGCGCGTTGGGAGTGGGGCCCGAGGTCCGGGTGGGCCTTTGCGTTCACCGG
GGCCTGGACATGGTGATCGGCATGATCGGCATCCTGAAGGCAGGGGGCGCCTATGTGCCG
ATGGACCCCACGTATCCCGCCGATCGGCTCGCCTTCATGCTCTCGGATTCGCAGGCGCCG
GTGGTGCTCACCGAAGAGCGGCTCCGGCAGCGCTTGCCGGACACGGGGGCGAAGGTGGTG
TGTCTGGATGCTCCGGAGGAGGGCTTTTCGGGCGCACTGGGCACCCCGCGAAGTGGGGTT
CACGCCGGAAACGTTGCCTATACCCTCTACACTTCGGGCTCCACGGGACGGCCCAAGGGG
GTCATGGTCTGCCACCGGAACGCGGAGAGCTTCTTCGCGGCGATGGATGCGCCCCTGGAC
AGCCAGACGCCAGGGACGTGGCTTGCGACGACGAGCATGTCGTTCGACATCTCCATTCTG
GAGATCCTGTTCTCCCTGACCCGTGGCTTCCAGGTCGTCATCCGAGGGGAGCAGGGGGCG
GGGTTGCCGGTCAGTGCCGGTCACCGCAAGGCGCCCCAGTTTAGCCTGTTCTACTTCGCC
AGCGACGAGCGGGAGAGCACCCACGGCAAGTATCAGCTCTTGCTGGAGGGGGCGCGGTTC
GCGGACCAGCATGGATTCACCGCCGTCTGGACGCCCGAGCGGCACTTCCACCCCTTTGGC
GGCATCTTTCCGAACCCTTCCGTCGTCAGTGCCGCCCTCGCGGCCACGACCCGGAACATC
CGGATCCGCGCGGGCAGCGTCGTGCTTCCGCTTCACAGCCCCATCCGTGTCGCCGAGGAG
TGGTCCATCGTGGACAACCTCTCGAATGGACGCGTCGATCTGTCCTTCGCCTCGGGATGG
CACCCGAACGACTTCGTGCTGGCCCCTGAGCGCTTTGCCGATGCGCGGAGCGGCCTGGCC
GGTCAGATCCAGACGTTCAAGAAGCTCTGGCGCGGAGAGAAGGTGAACTTCCGCAATGGG
GTGGGCACCGACGTCGCGGTTCAAGCGCTCCCTCGCCCGATTCAGCCGGACGTCGCGGTG
TGGTTAACCGCCGCGGGCAATCCCGAGACCTTCCGTTTGGCGGGAGAGCTTGGGACGAAC
ATCCTCACCCACCTGCTCGGCCAGAACCTCACGGAGCTGGAGAAGAAGATCCAGATCTAC
CGGGACGCTTGGAAGGCCGCGGGCCATGGCCCTGGAGAAGGCCACGTCACCCTCATGCTG
CACACGTTCGTGGGGGACGACGCCGCGGAGGTCCGCCAGAAGGTGCAAGGACCGCTTCGC
CAGTACCTGAAGAGCTCCGTGGGCCTGCTCAAGACCGTCATCGGTCCGCTGGCGCACGGT
GCGGAGTTCGAGTCTCTCAGTGAAGCGGACATCGATGTGCTGCTGTCGAGGGCGATTGAA
CGGTACCAGGATCAGATGGGCCTGTTTGGAACCCCCGAGAGCTGTTTGCCGATGGTCGCC
AAGCTCCGGGACCTCGGGGTGGATGAGATTGCCTGCCTGATCGACTTCGGCGTGGACCGC
GAGTCCACGCTGGCGGGGCTCCATCACCTGAACGAGCTGCGCGAGCGGAGCACCCAGCAC
GGTGAGCCCGAGGACATCCCCGCACTGGTGGCCCGGCACGGTGTGACCCACTTCCAGTGC
ACGCCCTCGATGCTGCGCATGCTGCTTCTGGAACCGGGCGGCACCGAGGCGCTTCGGCCG
CTGAAGAAGCTCCTCATTGGAGGCGAGGCCTTCCCCGCGGCGCTGGGGCAACAGGTCCGT
CCCCTGGTGGCAGGCGAGGTGCTCAACATGTATGGGCCGACCGAGACGACCATCTGGTCG
TCCTTCCATCGGTTGAGGCCTGATGAGGCCACGCTGCCCATCGGCCGGCCCATCCTGAAC
ACCCAGATGTACTTGCTGGATCGCCACCTTCAACCCGTTCCCATGGGGGTTCCCGGAGAG
CTGCTCATCGGAGGTGAGGGGGTGGCCCGGGGCTACCTCGACCGTCCCGAGCTGACGGCC
ACACGGTTCATCCCCGATCCCTTCGGTGCCGAGCCGGGGGCCCGTCTGTACAGGACCGGC
GATCTCGCGCGGTACCTTCCCGATGGACGCATCGAGTTCCTGGGCCGCATGGACCAGCAG
GTCAAGGTCCGTGGGGTTCGCATCGAGCCCGGGGAGATCGAATCCGCGCTGCGCCTGCAC
CCGGAGATCCGGCAGGCGGCGGTGGTGGCCCGGGCGGATGCGGCGGGCGAGGTGAGCCTG
GCTGCTTATGTCGTGGCAGGTCCAGACGCTCAGGTGGCCCCCTCGGAGCTGCGGCGCTTC
TTGAAGGACAAGCTGCCGGTCTCGATGATCCCCGATCACTTCATTCGGCTCGATGCGTTG
CCGCTGACGCCGAACAAGAAGCTGGATGTCCGGGCCTTGCCCGCCCCCGACGCGCCCCCG
GTGGAGCTTTCGGTGGCGTACGTCGCGCCACGGGATGCGTTGGAGTTGGAGCTCGTCGCG
CTCTGGGAAGAGCTGTTCGATCTGCGTCCCATCGGCGTGGCCAGCAGCTTCTTCCAGCTG
GGAGGTCACTCCTTGCTGGCGGTCCGGTTGATGTCCCGGTTGCGAGCGAAGTTCGGCCAG
CAGTTGCCCGTCTCGCTTCTGTTTCAGGCCGACACCATCCAGAAGCTGGCCTCCGTGCTG
CGCCAGGAGGGCCTCGCTGCGGCCCGCACGCCGCTCGTGCGCATCCAAGAGACGGGCGAT
CAACCGCCCCTCTTCTTCATGCATCCGACGGGTGGGGACGTGCTCTGTTATGCCCCCCTG
GCCAGGCAGCTTGGTCCCCGGCAGCCGTTCTATGCGCTGCAAGCGGTGGTGGATCAGGAG
GCCGAGTCCATCGAGGCGATGGCGGCGCGCTACCTGGAAGAGGTGCGAAAGGTCCGTCCC
AAGGGCCCCTATCGTCTGGGAGGTTGGTCCACGGGAGGGATCCTCGCGCAGGCCATGGCC
CGCCAACTGGAAGAGGCGGGGGAGCAGGTCGAGCTGCTCATGCTCCTGGAAACCTGGTCT
CCCACGGTCTATCAGCGGTCGGAGGGGACCACTGCCCTCATGGCGTGGTTCGCCACGGAT
CTGCTCGGAGGGGCCGGGGCGGCGCAGCTCGAGGCGTCCAAGCTCGAGACGCTCGACGAG
GCGGCGCAGCTCGACTACCTGTTCGAGCGTGCGAGTGCCTCGGGGGCGCTGCCCGGGGTG
GAGCGCTCCGAGATGGAGCAGCGGTTCCGGATCTTCGCCAAGACTGCCCGGGCGCTGTCC
CGGTACCAGTCTGACGCCTACCGGGGCAAGGTTCTCTTCTTGCAAGCGGAGGAGGCGGCC
ACCCCCACGGCGGGGACTCTTCCGGAGCCGCAGGCCAGCTGGGGCCAATCCCTGGAGCAG
GTTCAGATGTACCGGATGCCAGGAAACCACTACACCATGCTGCAAAGCCCCCACGTGCGT
GCGGTCGCTGACCGGATGGCCCGTGCCCTGGAGCAGCTCAGCGCGTCCGCTGAGCCACCC
CCGGGGGCGGTCCGAGCCGCATCCGCGCGGTAG
[8] C55..351
[8] A548..739
[8] Ox760..1069
[8] A1098..1308
[8] PCP1394..1461
[8] TE1483..1729
[8] C163..1053
[8] A1642..2217
[8] Ox2278..3207
[8] A3292..3924
[8] PCP4180..4383
[8] TE4447..5187

close this sectionFeature

BLASTP
Database:UniProtKB:2010_04 		show BLAST table
InterPro
Database:interpro:26.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[548-739] 2.70000000000002e-44 PF00501 [1098-1308] 1.80000000000002e-64 PF00501
PF00501   AMP-binding
IPR001031 Thioesterase (Domain)
[1483-1729] 3.7e-26 PF00975
PF00975   Thioesterase
IPR001242 Condensation domain (Domain)
[55-351] 7.90000000000016e-70 PF00668
PF00668   Condensation
IPR006163 Phosphopantetheine-binding (Domain)
[1397-1458] 2.1e-09 PF00550
PF00550   PP-binding
IPR009081 Acyl carrier protein-like (Domain)
[1394-1461] PS50075
PS50075   ACP_DOMAIN
IPR010071 Amino acid adenylation (Domain)
[548-1308] 1.19999063421924e-111 TIGR01733
TIGR01733   AA-adenyl-dom
IPR011251 Luciferase-like (Domain)
[752-1092] 2.3e-73 SSF51679
SSF51679   Luciferase_like
[753-1092] 1.39999277195138e-72 G3DSA:3.20.20.30
G3DSA:3.20.20.30   Luciferase_like
IPR016048 Luciferase-like, subgroup (Domain)
[760-1069] 5.30000000000001e-46 PF00296
PF00296   Bac_luciferase
SignalP No significant hit
TMHMM No significant hit
Myxo_00060 Myxo_00060   last last
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