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CDS information : Nanch_00160

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	67,823 / 68,863 / + [in whole cluster] 67,823 / 68,863 / + [in contig]
Location	67823..68863 [in whole cluster] 67823..68863 [in contig]
Type	CDS
Length	1,041 bp (346 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	NanG4
Gene
Gene (GenBank)	nanG4
EC number
Keyword	4-O-methyl-L-rhodinose
Note
Note (GenBank)	NDP-D-glucose-4,6-dehydratase; NDP-D-glucose-4-epimerase; NDP-D-glucose-4-reductase
Reference	ACC Q7WTE7 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
Related Reference	ACC Q9L4S7 NITE Urd_00230 PmId [11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998)

Sequence

>putative NDP-hexose C-4 ketoreductase [NanG4]
MAASPVPPPRGDEALAGTPVLVLGGSGYLGRHICSAFGAAGAQVVPVSRGARGGVDGDGC
RSVRLDLTAAGPDELARLCAGTGARVLVNASGAVWGGGERQMAEANTELVGRLAGAVARL
PGRPRLIHLGSAYEYGPARPGTAIAEDWPPAPTTVYGRTKLSGSQAVLRAAAELGVAGTV
LRVSVACGPGAPVSSLAGAVAAHLAAGRDELRLAPLRDHRDLVDVRDVADAVVAAAVAPV
AAVTGTVVNIGSGQAVPVRRLVDLMIALSGRPVRVIEDPALRRTRSDAAWQRLDIGRARR
LLGWAPRRTLRESLRDLLAAVGAPQPAAVRAATAIGPRNSHGKDSR

>putative NDP-hexose C-4 ketoreductase [NanG4]
ATGGCCGCCTCTCCGGTGCCCCCGCCCCGCGGTGACGAAGCGCTGGCCGGCACGCCGGTC
CTGGTCCTGGGCGGCTCCGGCTACCTGGGGCGGCACATATGCTCGGCGTTCGGTGCCGCG
GGCGCCCAGGTGGTGCCGGTCTCGCGCGGCGCGCGCGGCGGCGTGGACGGTGACGGCTGC
CGCTCGGTGCGCCTGGATCTGACCGCGGCCGGGCCCGATGAGCTGGCCCGGCTGTGCGCC
GGTACAGGGGCGCGGGTGCTGGTGAACGCCTCGGGCGCGGTCTGGGGCGGTGGCGAACGG
CAGATGGCCGAGGCCAACACCGAGCTGGTCGGCCGGCTGGCCGGGGCCGTCGCCCGATTG
CCGGGCCGGCCGCGGCTGATTCACCTGGGCAGCGCCTACGAGTACGGCCCGGCCCGCCCG
GGGACCGCGATCGCGGAGGACTGGCCGCCCGCCCCGACCACCGTCTACGGCCGCACCAAG
CTGAGCGGCTCGCAGGCCGTCCTTCGGGCCGCCGCCGAGCTCGGTGTCGCCGGAACCGTG
CTGCGGGTCTCCGTCGCCTGCGGCCCGGGCGCCCCGGTGAGCAGCCTCGCCGGGGCGGTG
GCCGCGCACTTGGCGGCCGGCCGCGACGAGTTGCGGCTCGCGCCCCTGCGCGACCACCGC
GACCTGGTGGACGTCCGGGACGTGGCCGACGCGGTGGTCGCGGCGGCCGTGGCACCGGTC
GCCGCCGTCACCGGCACAGTCGTCAACATCGGCAGCGGCCAGGCGGTGCCCGTGCGCCGG
CTGGTCGATCTGATGATCGCCCTCAGCGGGCGCCCGGTGCGTGTCATCGAGGACCCCGCG
CTGCGCCGGACGCGTTCCGACGCGGCCTGGCAGCGACTGGACATCGGACGTGCGCGGCGC
CTGCTGGGCTGGGCGCCGCGCCGAACCCTGCGGGAGTCACTGCGCGATCTGCTCGCCGCC
GTCGGCGCGCCGCAGCCCGCGGCGGTACGAGCGGCGACAGCGATCGGACCCCGGAACAGT
CATGGGAAGGACAGCAGATGA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro Database:interpro:26.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) SSF51735 NAD(P)-bd G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Nanch_00150 Nanch_00170

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