close all open/close all

CDS information : Ncarz_00240


close this sectionLocation

Organism
StrainATCC 15944
Entry nameNeocarzinostatin
Contig
Start / Stop / Direction43,345 / 44,670 / + [in whole cluster]
43,345 / 44,670 / + [in contig]
Location43345..44670 [in whole cluster]
43345..44670 [in contig]
TypeCDS
Length1,326 bp (441 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative monooxygenase
Product (GenBank)hydroxylase
Geneorf34
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005)
Related Reference
ACC
B5M9L6
NITE
Lasal_00200
PmId
[22506807] Sequential enzymatic epoxidation involved in polyether lasalocid biosynthesis. (J Am Chem Soc. , 2012)
[23796908] Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton. (Curr Opin Chem Biol. , 2013)
ACC
B5M9L6
NITE
Lasal2_00240
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
ACC
Q846W9
NITE
Monen_00220
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)

close this sectionSequence

selected fasta
>putative monooxygenase [hydroxylase]
MSGYVGDRAVVLGGSIAGSLAARVLADAYREVTVVDRDDLTDRAEPRHATPQGHHIHALL
ARGQQILDEIFPGFTDELVALGVPTGDFGTSLTWYFKGIAIKKAETGLTCVAAGRPLLEN
RIRHRIRRLPNVTLREQTDVLGLVTSPNRRTVTGVRVQSRSHNSAEEVLAADLVVDTTGR
GTRTPRWLAELGYPQVLEERVKMDLTYTTCHFRGPLPFDPIGEDIALLPVATPAMRRGAI
FARMPDRYSLSLTGVLGDRPPTDHEGFLSYAESLPIPEVYKALRDAEPLGPPTSFHFPAS
IRRRYERQARHLGGLLVMGDAACVFNPVYGQGMSVAAIQSLVLRDHLLQGPPEPRAFFRD
LAGAIDGPWDMSAGADLGFPEVQGRRTAKTRLGNAYVTRLQTAAAADRELSKAFLRAAGL
VDPPQALMRPRLISRVLRGAR
selected fasta
>putative monooxygenase [hydroxylase]
ATGAGTGGCTATGTCGGAGATCGTGCCGTGGTCCTGGGAGGCAGCATCGCAGGGTCCCTG
GCGGCACGCGTACTCGCCGACGCCTACCGCGAGGTGACCGTGGTCGACCGGGACGACCTC
ACCGACCGTGCCGAGCCGCGACACGCCACCCCCCAGGGACACCACATCCACGCGCTCCTC
GCCCGTGGTCAGCAGATCCTCGATGAGATCTTCCCCGGCTTCACCGACGAACTGGTCGCC
CTCGGAGTACCCACCGGTGACTTCGGCACCAGCCTGACCTGGTATTTCAAGGGAATCGCG
ATCAAGAAGGCAGAGACGGGGCTGACGTGTGTGGCGGCCGGCCGGCCGCTGCTCGAGAAC
CGAATCCGGCACCGGATCCGGCGCCTGCCCAACGTCACGCTGCGGGAGCAGACGGACGTA
CTGGGGCTCGTGACATCACCGAACCGCCGCACCGTGACCGGGGTCCGGGTGCAGAGCCGT
TCGCACAACTCCGCTGAGGAGGTGCTCGCCGCGGACCTCGTGGTCGACACGACCGGGCGC
GGCACGCGGACCCCGCGATGGCTGGCAGAGCTCGGCTATCCGCAAGTCCTTGAGGAACGG
GTGAAGATGGACCTGACCTATACGACGTGCCACTTCCGCGGTCCTCTGCCGTTCGACCCG
ATCGGCGAAGACATCGCCCTTCTGCCAGTCGCCACCCCGGCCATGCGGCGAGGGGCGATC
TTCGCCCGGATGCCCGACCGCTACTCACTGTCGCTCACAGGGGTCCTCGGCGACCGGCCC
CCCACCGACCACGAGGGATTTCTCTCCTACGCCGAGTCCCTGCCGATTCCAGAGGTGTAC
AAGGCCCTGCGGGACGCGGAGCCGCTCGGTCCTCCCACGTCGTTCCACTTCCCGGCCAGC
ATCCGCCGCCGCTACGAACGGCAGGCGCGCCATCTGGGGGGACTGCTCGTGATGGGCGAT
GCGGCCTGCGTCTTCAACCCGGTGTACGGGCAGGGCATGTCGGTGGCCGCGATCCAGTCG
CTCGTACTGCGTGACCACCTGCTCCAGGGGCCGCCCGAGCCGCGCGCCTTCTTCCGTGAT
CTCGCCGGCGCTATCGACGGGCCCTGGGACATGTCCGCGGGAGCGGACCTCGGTTTTCCG
GAGGTTCAGGGGCGGCGAACGGCGAAGACTCGGCTCGGCAACGCCTACGTCACACGCCTT
CAGACCGCTGCGGCCGCCGACAGGGAGCTGTCCAAGGCCTTCCTGCGCGCCGCCGGGCTC
GTCGACCCGCCCCAGGCGCTCATGCGACCGCGGCTGATCTCTCGCGTCCTGCGCGGCGCT
CGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
No significant hit
SignalP
 [1-26]  0.892 Signal
Eukaryota   
 [1-28]  0.289 Signal
Bacteria, Gram-positive   
TMHMM No significant hit