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CDS information : Niger_00070


close this sectionLocation

Organism
StrainDSM 4137
Entry nameNigericin
Contig
Start / Stop / Direction52,139 / 57,244 / + [in whole cluster]
52,139 / 57,244 / + [in contig]
Location52139..57244 [in whole cluster]
52139..57244 [in contig]
TypeCDS
Length5,106 bp (1,701 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)NigAVI
Gene
Gene (GenBank)nigAVI
EC number
Keyword
Note
Note (GenBank)
  • modular polyketide synthase; ORFF
Reference
ACC
PmId
[17584617] Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (Chem Biol. , 2007)

close this sectionPKS/NRPS Module

9 methylmalonyl-CoA
KS35..417
AT587..909
kr1226..1405
ACP1542..1616

close this sectionSequence

selected fasta
>polyketide synthase [NigAVI]
MSGTEEKLRQYLKRVTVDLGQARQRLREMEERSQEPVAIVSMACRYPGGVGCPEELWELV
ASGGDGITAFPTDRGWDLDGLYHPDPDHPGTSYVRHGGFLGGTDRTDGTDRFDAEFFGIS
PREALAMDPQQRLLLEVSWELFERAGVDPVTTKGSRTGVYAGVSSQDYLSRMPRVPEGFE
GYATTGSLTSVVSGRVAYTFGLEGPAVTVDTACSSSLVAMHLAAQALRQGECDLALAGGV
TALTTPTAFAEFSRQRGLAPDGRCKSFAAGADGTGFSEGVGLVLLERLSDARRNGHRVLA
LIRGSAINQDGASNGLTAPNDVSQERVIRQALAGARLAPDQVDAVEAHGTGTSLGDPIEA
HALLATYGQDRPGERPLWLGSLKSNIGHAQAAAGVAGVIKMVMALRHETLPVTLHIDEPT
PHVEWEGGGVRLLTEPVPWPTGERPRRAGVSSFGISGTNAHLILEQAPEPSSPAASEEAV
PEEPAAATPEESGTAMVPWVLSARGAQALRGQAAALVRRTADEPGPSIADVSWSLATTRS
VFDHRAVAVGETRAELVAAVEALATGGTHPGLVHSGGAAVAGELGPVLVFPGQGSQWPGM
GAELLSTSAVFAARVAECERALAPYVDWSLTDVLRGADGAADLGRVDVVQPVLWAVMVSL
AAVWAGHGVRPAAVIGHSQGEIAAAVVAGALSLEDGAKVVALRSKALRRLAGGGAMASLG
VSRERARTLLAGLGDRAVAVGVAAVNGPSSTVISGPPEQVAHVVAACREAGDRARPIEVD
YASHSPQVDEIADELAEALAGVRPVASAVAFYSTVTVGRIDTTRLDTGYWVANLREPVRF
ADTVQALLADGHRVFIEGSTHPVLTVGMQESFEEAGVEAVTVPTLRRDHGGQARLLQSLA
LAFTAGVAVDWTTLHPTGPAAPRTVELPTYAFQRERYWLADLVSFDTASPADEEESRFWA
AVEGEDLGALSETLNLPDGTGHRTSLGTVLPALSTWRRGRRERSAVDSWRHRVEWRPVPD
TAPTTAGSWLVVHPKSASDGWTDACVRALGADGGQVYLLELDGDTDREGLAALLRSRYAE
ETPPTGVLSLLALDEGTPPLPGVAPGLTGTLTLLQALVDAAVPAPLWCATRGAVSIDDSD
PLDAPHQAQLWGLGRVAALEHPDRWGGLVDLPASPDSLDARRLCALLTGACGEDEAALRP
AGAYGRRLIPDPIGGRAPRRIWKPRGTVLITGAADGPAAHVARCLAADGAAHIVMLGPGG
GDAPAAVELKAELDALGTGLTVADHAPADREATAHLLERVAQASGRIGTIVHTAASGELA
PLMELTPRRLAEEIHAHLDDTGRPLDELCGMGPEDTVVYFSTVAASWGSKDHGSYAAATA
CLDALARRDRAEGRPTVSIAWGLWDLPYGGDATDVPYGGDTPDAPYGGDTPDTPDTPDAA
ETPSAPHTERSRRQGLSPLDPRLALTALRQALDHDDPSVTIADVRWERFAPLFTLARPSR
LFDGVLAARQAIETARGPVEDTVGDEASALRRELGALPEDERGERLLALVRAHVAAVLHY
PAPESVEPDRPFKELGFDSIAAVELRNRLRAATGLSLPTTVVFDYPTPRTLAGHLLTEVD
PGEAGAAAHPVSGHLDELEAALAGLSAGDPRRAGLVNRLQALVWKYTATARESDEPEADE
QDLTAATADEVFALIDRELGV
selected fasta
>polyketide synthase [NigAVI]
ATGTCGGGTACGGAAGAGAAACTCCGCCAGTATCTGAAGCGGGTCACGGTGGATCTCGGG
CAGGCCCGTCAGCGGCTGCGCGAGATGGAGGAGCGCTCCCAGGAGCCAGTGGCCATCGTG
AGCATGGCCTGCCGCTATCCCGGTGGTGTCGGCTGCCCCGAGGAGCTGTGGGAGCTGGTG
GCGTCCGGTGGCGACGGCATCACCGCGTTCCCCACCGATCGCGGCTGGGATCTGGACGGC
CTCTACCATCCGGACCCCGACCACCCCGGCACCAGCTACGTCCGGCACGGCGGATTCCTG
GGCGGTACGGACCGTACGGACGGCACCGACCGTTTCGACGCGGAGTTCTTCGGGATCAGC
CCGCGCGAGGCCCTGGCGATGGATCCACAGCAGCGGCTGCTGCTGGAGGTGTCCTGGGAG
CTGTTCGAGCGCGCGGGTGTCGATCCGGTGACGACGAAGGGCAGTCGGACCGGGGTGTAC
GCGGGCGTGTCCAGCCAGGACTATCTGTCCAGGATGCCCCGGGTCCCCGAGGGCTTCGAG
GGCTATGCCACGACCGGCAGCCTCACCAGTGTGGTCTCCGGACGGGTGGCGTACACCTTC
GGCCTGGAGGGCCCGGCGGTGACGGTGGACACGGCGTGTTCGTCGTCGCTGGTGGCGATG
CATCTGGCGGCGCAGGCGCTGCGGCAGGGCGAATGCGATCTGGCGCTCGCCGGCGGAGTC
ACCGCGCTCACCACACCGACCGCCTTCGCCGAGTTCTCCCGGCAGCGCGGGCTGGCCCCG
GACGGCCGCTGCAAGTCCTTCGCGGCGGGCGCGGACGGCACGGGCTTCTCCGAGGGCGTC
GGCCTGGTCCTGCTGGAGCGGCTCTCCGACGCGCGGCGCAACGGACATCGGGTACTGGCC
CTGATCCGGGGCTCGGCCATCAACCAGGACGGCGCCAGCAACGGACTGACCGCGCCCAAC
GACGTATCGCAGGAACGGGTGATCCGCCAGGCGCTGGCCGGCGCACGCCTCGCCCCCGAC
CAGGTCGACGCGGTGGAGGCCCATGGCACCGGAACCTCCCTCGGCGACCCCATCGAGGCA
CACGCCCTGCTGGCCACCTACGGACAGGACCGGCCCGGTGAACGGCCCCTGTGGCTGGGC
TCGCTCAAGTCCAACATCGGCCACGCCCAGGCGGCCGCTGGTGTGGCCGGTGTGATCAAG
ATGGTGATGGCGCTCCGGCACGAGACGCTGCCGGTCACCCTGCACATCGATGAGCCGACC
CCGCATGTGGAGTGGGAGGGCGGCGGGGTACGGCTGCTGACCGAGCCGGTGCCGTGGCCG
ACGGGCGAACGCCCCCGCAGGGCCGGGGTGTCCTCCTTCGGGATCAGCGGCACCAACGCC
CATCTGATCCTGGAACAGGCCCCCGAGCCCAGCTCCCCGGCCGCATCAGAGGAGGCGGTG
CCGGAGGAGCCAGCCGCCGCCACGCCCGAGGAGTCGGGGACGGCGATGGTGCCCTGGGTG
CTGTCCGCCCGTGGCGCCCAGGCCCTGCGGGGGCAGGCGGCCGCGCTCGTCCGGCGGACA
GCCGACGAACCGGGGCCGTCCATCGCCGATGTGAGCTGGTCGCTGGCCACCACCCGGTCG
GTGTTCGACCACCGGGCCGTGGCCGTGGGCGAGACCCGTGCGGAACTGGTGGCGGCCGTG
GAGGCGCTGGCCACCGGCGGAACGCACCCGGGCCTGGTCCACTCCGGTGGTGCCGCGGTG
GCCGGGGAGCTCGGCCCGGTGCTGGTGTTCCCCGGCCAGGGCTCACAGTGGCCGGGCATG
GGCGCCGAACTGCTCAGCACCTCGGCCGTGTTCGCGGCTCGCGTGGCGGAGTGCGAACGG
GCGCTGGCACCGTACGTCGACTGGTCCCTCACCGATGTGCTGCGAGGTGCCGACGGGGCG
GCCGACCTGGGCCGGGTCGATGTGGTGCAGCCCGTCCTGTGGGCCGTGATGGTGTCGCTC
GCCGCGGTGTGGGCGGGTCACGGCGTACGTCCCGCGGCGGTGATCGGGCACAGCCAGGGC
GAGATCGCGGCGGCCGTGGTGGCCGGGGCCCTGTCGCTGGAGGACGGTGCCAAGGTCGTG
GCGCTGCGCAGCAAGGCGCTGCGGCGGCTCGCCGGTGGCGGGGCGATGGCCTCCCTGGGG
GTGAGCCGGGAGCGGGCCCGTACGCTGCTGGCCGGGCTGGGCGACCGGGCGGTGGCCGTC
GGGGTGGCGGCCGTCAACGGCCCCTCGTCCACGGTGATTTCGGGGCCGCCCGAGCAGGTG
GCGCATGTCGTGGCGGCGTGTCGGGAGGCGGGCGACCGGGCCCGGCCGATCGAGGTGGAC
TACGCCTCGCACAGCCCCCAGGTGGACGAGATCGCCGACGAGCTGGCGGAGGCTCTGGCG
GGTGTCCGGCCGGTCGCGTCCGCGGTGGCGTTCTACTCCACGGTCACCGTCGGCCGGATC
GACACCACCCGGCTGGACACCGGTTACTGGGTTGCCAACCTCCGTGAGCCGGTGCGGTTC
GCCGACACCGTTCAGGCGCTGCTGGCCGACGGCCACCGCGTGTTCATCGAGGGCAGCACC
CACCCCGTGCTGACCGTGGGAATGCAGGAGAGCTTCGAGGAAGCCGGGGTCGAGGCGGTC
ACCGTACCCACGCTCCGCCGCGACCACGGCGGCCAGGCGCGGCTGCTCCAGTCCCTCGCG
CTGGCCTTCACGGCGGGTGTCGCGGTCGACTGGACCACGCTCCACCCCACCGGTCCGGCC
GCCCCGCGCACCGTCGAACTGCCCACCTACGCCTTCCAGCGCGAGCGGTACTGGCTGGCC
GACTTGGTGTCCTTCGACACCGCGTCCCCGGCGGACGAGGAGGAGTCCCGGTTCTGGGCC
GCGGTGGAGGGCGAGGACCTGGGCGCGCTCTCCGAGACGCTGAACCTCCCGGACGGCACC
GGCCACCGGACCTCGCTGGGCACCGTGCTGCCCGCGCTCTCCACATGGCGTCGCGGGCGG
CGTGAGCGCTCCGCCGTGGACTCCTGGCGCCACCGCGTCGAGTGGCGGCCCGTCCCCGAC
ACCGCCCCCACCACCGCGGGCTCCTGGCTCGTGGTCCACCCGAAGAGTGCCTCCGACGGC
TGGACGGACGCCTGCGTACGGGCGCTGGGCGCGGACGGCGGCCAGGTGTACCTGCTGGAA
CTGGACGGGGACACCGACCGGGAGGGCCTCGCCGCGCTGCTGCGCTCCCGGTACGCCGAG
GAGACACCACCGACCGGCGTACTGTCGCTGCTGGCCCTGGACGAGGGCACACCGCCTCTC
CCCGGTGTGGCCCCCGGCCTCACCGGCACGCTCACCCTGCTCCAGGCGCTGGTGGACGCG
GCGGTCCCCGCGCCCCTGTGGTGCGCCACCCGCGGCGCGGTCTCCATCGACGACTCCGAC
CCCCTCGACGCTCCCCACCAAGCCCAGCTGTGGGGGCTCGGCAGAGTGGCCGCCCTGGAA
CACCCCGACCGTTGGGGTGGGTTGGTGGACCTGCCCGCGAGCCCGGACAGCCTGGACGCG
CGTCGGCTGTGTGCCCTGCTCACCGGGGCCTGTGGCGAGGACGAGGCGGCGCTGCGCCCG
GCGGGAGCGTATGGACGTCGGCTGATTCCCGACCCGATCGGCGGCCGCGCCCCGCGGCGC
ATCTGGAAACCCCGGGGCACCGTGCTCATCACCGGAGCCGCCGACGGACCCGCCGCCCAT
GTCGCCCGCTGCCTTGCCGCCGACGGCGCGGCACACATCGTGATGCTGGGCCCCGGAGGC
GGCGACGCGCCCGCCGCCGTGGAGCTGAAGGCCGAACTCGACGCGCTCGGCACCGGACTC
ACCGTGGCGGACCACGCGCCCGCCGACCGGGAAGCCACCGCACACCTCCTCGAGCGGGTG
GCGCAGGCGAGCGGACGGATCGGGACGATCGTCCACACCGCGGCCTCCGGTGAGCTGGCC
CCGCTGATGGAACTCACCCCGCGGCGGCTGGCCGAGGAGATCCACGCCCATCTGGACGAC
ACCGGGCGGCCATTGGACGAACTGTGCGGCATGGGTCCCGAGGACACCGTGGTCTACTTC
TCCACCGTCGCCGCGTCCTGGGGCAGCAAGGACCACGGCTCCTACGCGGCCGCCACCGCC
TGCCTCGACGCACTGGCGCGGCGCGACCGGGCCGAGGGCCGGCCCACCGTCTCGATCGCG
TGGGGCCTGTGGGACCTGCCCTACGGCGGAGACGCAACGGATGTGCCGTACGGGGGTGAC
ACTCCAGACGCGCCGTACGGTGGTGACACCCCGGACACCCCGGACACCCCGGACGCCGCA
GAAACCCCGTCGGCCCCGCACACCGAGCGGTCCCGGCGGCAGGGCCTGTCCCCGCTGGAC
CCACGGCTGGCGCTCACCGCACTGCGCCAGGCGCTCGACCACGACGATCCGAGCGTCACG
ATCGCCGATGTGCGATGGGAGCGCTTCGCGCCGCTGTTCACCCTCGCCCGCCCCAGCAGG
CTCTTCGACGGTGTCCTGGCCGCCCGCCAGGCCATCGAGACCGCGCGGGGCCCCGTGGAG
GACACCGTCGGCGACGAGGCGTCGGCGCTACGGCGGGAGCTGGGCGCCTTGCCGGAGGAC
GAGCGGGGCGAGCGGCTGCTGGCGCTGGTACGGGCCCATGTGGCCGCCGTACTGCATTAC
CCGGCGCCGGAGTCGGTCGAGCCGGACCGCCCGTTCAAGGAGCTGGGGTTCGACTCCATC
GCGGCCGTCGAACTCAGGAACCGGCTGCGCGCCGCCACGGGGCTGAGCCTCCCGACCACG
GTGGTCTTCGACTATCCGACACCTCGGACGCTGGCGGGCCATCTGCTGACCGAGGTGGAC
CCGGGGGAGGCCGGGGCGGCAGCACATCCGGTGTCCGGACACCTCGACGAGCTCGAGGCC
GCACTGGCCGGGCTGTCGGCCGGCGACCCGCGGCGGGCCGGTCTGGTGAACCGGTTGCAG
GCGCTGGTGTGGAAGTACACCGCCACCGCGCGGGAGTCCGATGAGCCGGAGGCGGATGAG
CAGGATCTGACGGCGGCCACCGCCGATGAGGTGTTCGCCCTCATCGACCGCGAACTCGGC
GTCTGA
[9] KS35..417
[9] AT587..909
[9] methylmalonyl-CoA781..785
[9] kr1226..1405
[9] ACP1542..1616
[9] KS103..1251
[9] AT1759..2727
[9] methylmalonyl-CoA2341..2355
[9] kr3676..4215
[9] ACP4624..4848

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [587-710]  7.69999999999997e-72 G3DSA:3.40.366.10 [781-898]  7.69999999999997e-72 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
 [1574-1589]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1550-1615]  9.30000000000002e-16 PF00550
PF00550   PP-binding
 [1545-1618]  6.40000000000001e-25 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1538-1633]  7.19999557654549e-24 SSF47336
SSF47336   ACP_like
 [1542-1616]  PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
 [1226-1405]  1.09999999999999e-35 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [35-291]  5.20000000000001e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [299-417]  4.39999999999997e-46 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [587-909]  6.99999999999989e-103 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [4-28]  3.5e-12 PF08990
PF08990   Docking
 [4-35]  5.50000546509999e-11 SSF101173
SSF101173   Polyketide_synth_docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [585-890]  3.39999972437719e-65 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [712-780]  4.79999776836274e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [37-303]  8.70000000000003e-89 G3DSA:3.40.47.10 [304-470]  2.79999999999994e-65 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [27-469]  8.70005082121738e-100 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1227-1412]  1.1e-19 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [204-220]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [589-889]  2.2999842048857e-118 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1547-1619]  4.39999001237724e-39 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [37-469]  SM00825
SM00825   PKS_KS
SignalP No significant hit
TMHMM No significant hit