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CDS information : Niger_00130


close this sectionLocation

Organism
StrainDSM 4137
Entry nameNigericin
Contig
Start / Stop / Direction65,980 / 65,513 / - [in whole cluster]
65,980 / 65,513 / - [in contig]
Locationcomplement(65513..65980) [in whole cluster]
complement(65513..65980) [in contig]
TypeCDS
Length468 bp (155 aa)
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close this sectionAnnotation

Category3.4 other modification
Productputative epoxide hydrolase/cyclase
Product (GenBank)NigBIII
GenenigBII
Gene (GenBank)nigBIII
EC number
Keyword
Note
  • Belongs to polyether epoxide hydrolase(PEH)-A group.
Note (GenBank)
  • cyclase; ORF7
Reference
ACC
PmId
[17584617] Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (Chem Biol. , 2007)
Related Reference
ACC
Q846W8
NITE
Monen_00230
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
[16632258] Evidence for the role of the monB genes in polyether ring formation during monensin biosynthesis. (Chem Biol. , 2006)
ACC
B6ZK72
NITE
Lasal_00210
PmId
[18710235] Epoxide hydrolase Lsd19 for polyether formation in the biosynthesis of lasalocid A: direct experimental evidence on polyene-polyepoxide hypothesis in polyether biosynthesis. (J Am Chem Soc. , 2008)
[21375229] Enzymatic epoxide-opening cascades catalyzed by a pair of epoxide hydrolases in the ionophore polyether biosynthesis. (Org Lett. , 2011)
[22388816] Enzymatic catalysis of anti-Baldwin ring closure in polyether biosynthesis. (Nature. , 2012)

close this sectionSequence

selected fasta
>putative epoxide hydrolase/cyclase [NigBIII]
MADEATLKRMALEYARRMNTGDVEAVLDLFSDDIVFEDPVGAPPLIGKDALREHIAWSIE
CQVHETPGRPVTSMDGRRVAVPTTVTVYAPAKLTFSIIGVIELGDDGLVHHAQAFWGITD
TKVGDGPELTGVAHFMAVTQNLAKMVQAKGSPSPM
selected fasta
>putative epoxide hydrolase/cyclase [NigBIII]
ATGGCCGATGAGGCGACCCTCAAGAGGATGGCTCTGGAATACGCGCGGCGCATGAATACA
GGCGATGTCGAAGCCGTATTGGACCTCTTCTCGGACGACATCGTCTTCGAGGACCCGGTC
GGCGCGCCCCCGCTCATCGGAAAGGACGCACTTCGCGAACATATCGCCTGGTCCATCGAA
TGCCAGGTGCATGAGACCCCGGGCCGTCCGGTCACCTCGATGGACGGCCGCAGGGTGGCG
GTGCCGACCACGGTCACGGTGTACGCACCGGCCAAACTCACCTTCAGCATCATCGGCGTG
ATCGAACTCGGTGATGACGGTCTGGTCCATCACGCCCAGGCGTTCTGGGGCATCACGGAT
ACGAAAGTGGGCGACGGCCCCGAACTCACCGGTGTCGCGCATTTCATGGCGGTCACCCAG
AACCTCGCCAAGATGGTCCAGGCCAAGGGCAGCCCCAGTCCGATGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR009959 Polyketide cyclase SnoaL-like domain (Domain)
 [13-110]  7.7e-12 PF12680
PF12680   SnoaL_2
SignalP
 [1-25]  0.077 Signal
Bacteria, Gram-positive   
TMHMM No significant hit