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CDS information : Niger_00150


close this sectionLocation

Organism
StrainDSM 4137
Entry nameNigericin
Contig
Start / Stop / Direction72,278 / 66,450 / - [in whole cluster]
72,278 / 66,450 / - [in contig]
Locationcomplement(66450..72278) [in whole cluster]
complement(66450..72278) [in contig]
TypeCDS
Length5,829 bp (1,942 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)NigAIX
Gene
Gene (GenBank)nigAIX
EC number
Keyword
Note
Note (GenBank)
  • modular polyketide synthase; ORFI
Reference
ACC
PmId
[17584617] Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (Chem Biol. , 2007)

close this sectionPKS/NRPS Module

14 methylmalonyl-CoA
KS34..410
AT575..896
DH946..1121
KR1492..1672
ACP1775..1845

close this sectionSequence

selected fasta
>polyketide synthase [NigAIX]
MTDDEKLRSYLKRATADLRLARRQLREVEDRAREPIAIIGIACHFPGDVATPEDLWRVVD
EGVDVISSFPEDRGWDLDSLYDPDPEHAGTSSAREGGFLRDVADFDAEFFGISPREAAAM
DPQQRLLLETAWEAFERAGLTREALNGSRTGVFAGVDSYHYLSLIGQTTGDSAGYVATGN
LGSVVSGRVSYSFGLEGPAVTVDTACSSSLVATHLAVQALRQDECSLALAGGVTVMATPG
GFTEFSRQRALSPDGRCKAFAAAADGTGFSEGVGLVLLERLSDARRNGHRVLAVIRGSAV
NQDGASNGLTAPNGPSQQRVIRQALANAGLSPSEVDAVEAHGTGTTLGDPIEAQALLATY
GQDRPEGRPLWLGSIKSNIGHTQAAAGVASVIKMVQALRHEVLPVSLHIDAPTPHVDWGA
GEVRLLSEPVEWAANEQPRRAGVSSFGISGTNAHLILEQAPAQPVEAARAPDSSRGGVPA
GGVVPWVVSGRGAKALRGQARALAERVATDPDASPAEVGWSLISTRSVFDHRAVIVGEHR
DELLAGLNALATGDTHPTLTEPTTTGPGAGGMGPVLVFPGQGSQWLGMGAGLLDTSPVFA
TRIAECEHALTPHVDWSLTEVLRGGVNAADLARVDVVQPVLWAVMISLAAVWDHHGLTPT
AVVGHSQGEIAAACVAGALSLDEGARIVALRAHALRRLAGHGAMASLTTSQTHTEELLTN
LGEQADAVGVAAVNGPGSVVISGPPEQVTHAITACEQAGHRARLIDVDYASHSAQVDEIA
EELNQLLAGVKPVSAQVAFYSTVTGARMDTSGLDTAYWIKNLRERVRFADAVRALLHDGH
RVFIEASTHPVLTIGMQESIEEARVDAVTIPTLRRDHGGPDQLARSVARAFTAGLSVDWT
RWFPTDPAPRTIDLPTYAFQRERYWLDGEGGPGGDPAGLGLTAAGHPLLGAAVELADGTT
HVLTGRISARSHPWLAEHVVAGAVLAPGAVLVEWALRAADAVGCGGVEELALQVPLALPE
SGGLRVQVVVGAAAEDGRREVRMYSSPDHPNSLDPGLDSGADTGWVCHAVGVLAPAAEAP
APVEGLGGAWPPPGAEPLDVGAFYEQVLAAGYGYGPAYQGLKAAWRQGGDVLAEVALPDA
AGGRDGYGIHPALLDAALHPSLLMDRPEGQEDDGQVWLPFAWNGVSLWATEAATVRVRLS
RDEERQSLRLTVADTVGAPVLTVDSLVTRPAAADQLCSAAAARAVDGLFTLDWMAVPAPA
TAPSPASVVGEGSWVVLGEDRLGLAEPVAAGLGADATVSHPDLEALIAAVDAGEPAPAVV
LTHPYTAEGGHGDGLGAVEELLGLVRSWLRRPALADARLVVVTRGAVAAGRAGDATDDAD
DTALDPSGVDPSGAGAWGLIRSAQSENPGRFVLLDLDEGHEGRAGLVEAVVRAVESHEPQ
MAVRDGRVLVPRMVRAMAAPGPTTDDRGESTTGLDENTTGLDGGATGLDTDGTVLITGGT
GTLGTLVAEHLVRAWGVRHLLLVSRSGPEAAGAGELTAALTELGAEVRIAAVDVTDPDAV
AALVAGVDSAHPLTGVVHAAGVLDDGVLTTQTPERLARVWRPKATAAAHLHAATAELPLS
LFVMFSSTAGTLGASGQSNYAAANAYCDALAARRHALGLPGLSVAWGLWADASGMTGHLG
EADRARMTRSGIGAMSSERALGLLDAAMRHGHHHLIAIDLDVRALTGRPALTLPAPLRAL
AGGGTARRTAATARPHTDWAGHLAALPLEEQRRTLLNLVLSHAAAALGHSDPGRIQTERG
FLELGFDSLTAIELRNRLTAVTGLRLATTLIFDHPNPAALAAHLHSELAPEDADPLAPML
GEIDRMESALLSVAQDETAHEALLERLRTTLSKLGALHGGGTEEGPAAGRIQDATADEIF
QFIDEDLGRSESNGEHAHGGTR
selected fasta
>polyketide synthase [NigAIX]
ATGACGGATGACGAGAAGCTCCGCAGCTATCTCAAGCGGGCGACCGCCGATCTGCGTCTG
GCCAGGCGGCAGTTGCGCGAGGTCGAGGACCGCGCCCGGGAGCCCATCGCCATCATCGGG
ATCGCCTGCCACTTCCCCGGCGATGTGGCGACCCCCGAGGACCTGTGGCGGGTGGTGGAC
GAGGGTGTGGATGTCATCTCCTCGTTCCCCGAGGACCGTGGCTGGGACCTGGACAGCCTC
TATGACCCGGACCCCGAGCACGCCGGTACCAGCAGCGCCCGCGAGGGCGGCTTCCTGCGC
GATGTGGCCGATTTCGACGCCGAGTTCTTCGGGATCAGCCCGCGGGAGGCCGCGGCGATG
GATCCGCAGCAGCGGCTTCTGCTGGAGACGGCGTGGGAGGCGTTCGAACGCGCCGGACTG
ACACGGGAGGCGCTGAACGGCAGCCGCACCGGGGTGTTCGCCGGAGTGGACTCGTACCAC
TACCTGTCGCTCATCGGCCAGACGACCGGTGACTCGGCGGGCTATGTGGCCACCGGCAAC
CTCGGCAGTGTGGTCTCGGGCCGGGTGTCGTACTCATTCGGTCTGGAGGGGCCCGCGGTC
ACGGTGGACACCGCGTGCTCGTCGTCGCTGGTGGCCACGCATCTGGCGGTGCAGGCGCTA
CGGCAGGACGAGTGCTCGCTGGCCCTCGCGGGCGGCGTGACGGTGATGGCCACGCCCGGC
GGATTCACCGAGTTCTCGCGTCAGCGCGCGCTGTCCCCGGACGGGCGGTGCAAGGCGTTC
GCCGCGGCGGCGGACGGCACGGGCTTCTCCGAGGGCGTCGGACTGGTGCTGCTGGAGCGG
CTCTCCGACGCACGGCGCAACGGACATCGGGTGCTGGCGGTGATCCGGGGGTCGGCCGTC
AACCAGGACGGGGCCAGCAACGGGCTCACGGCGCCCAACGGGCCCTCGCAGCAGCGGGTG
ATCCGGCAGGCGCTGGCCAACGCGGGACTGTCGCCGTCCGAGGTGGACGCGGTGGAGGCA
CACGGAACGGGCACGACACTGGGTGACCCCATCGAGGCGCAGGCGCTGCTGGCCACCTAC
GGACAGGACCGGCCGGAGGGACGTCCGCTGTGGCTCGGCTCGATCAAGTCCAATATCGGG
CATACGCAGGCCGCCGCCGGAGTGGCGAGCGTCATCAAGATGGTGCAGGCCCTGCGCCAC
GAGGTGCTGCCCGTCTCGCTCCACATCGACGCGCCGACACCGCATGTGGACTGGGGCGCG
GGCGAAGTGCGGCTGCTGTCGGAACCGGTCGAGTGGGCGGCGAACGAACAGCCGCGCCGT
GCGGGTGTGTCGTCGTTCGGCATCAGCGGTACGAACGCCCATCTGATCCTTGAGCAGGCA
CCCGCCCAGCCGGTGGAGGCCGCCCGCGCACCGGACAGTTCGCGGGGCGGTGTTCCGGCG
GGTGGTGTGGTGCCGTGGGTGGTGTCCGGGCGCGGCGCCAAGGCACTGCGCGGTCAGGCG
CGGGCGCTGGCCGAACGCGTGGCCACCGACCCCGACGCCTCCCCGGCGGAGGTCGGCTGG
TCGCTGATCAGCACCCGCTCCGTCTTCGACCACCGCGCCGTCATCGTCGGCGAACACCGC
GACGAACTCCTCGCCGGACTCAACGCCCTCGCCACCGGCGACACACACCCCACCCTCACC
GAACCAACCACCACCGGTCCCGGCGCGGGCGGCATGGGACCCGTCCTGGTCTTCCCCGGC
CAAGGCTCCCAATGGCTCGGCATGGGCGCCGGACTCCTGGACACCTCACCCGTCTTCGCC
ACCCGCATAGCCGAATGCGAACACGCCCTCACCCCCCACGTCGACTGGTCCCTCACCGAG
GTTCTGCGCGGCGGCGTCAACGCAGCCGACCTCGCCCGCGTCGACGTCGTCCAACCCGTC
CTATGGGCCGTCATGATCTCCCTGGCCGCCGTATGGGACCACCACGGCCTCACCCCCACC
GCCGTCGTCGGACACTCCCAAGGCGAAATCGCCGCAGCCTGCGTCGCCGGAGCCCTCTCC
CTCGACGAAGGCGCCCGCATCGTCGCACTCCGCGCCCACGCCCTCCGACGCCTCGCCGGA
CACGGCGCCATGGCCTCCCTCACCACAAGCCAAACCCACACCGAAGAACTCCTGACCAAC
CTCGGCGAGCAGGCCGACGCGGTGGGCGTGGCGGCGGTCAACGGACCGGGGTCCGTGGTG
ATCTCCGGCCCACCCGAACAAGTCACCCACGCCATCACCGCATGCGAACAAGCAGGCCAC
CGAGCCCGGCTCATCGACGTGGACTACGCCTCCCACAGCGCCCAAGTAGACGAAATCGCC
GAAGAGTTGAATCAGCTCCTGGCCGGGGTCAAGCCGGTGTCGGCCCAGGTGGCGTTCTAT
TCGACCGTCACCGGCGCCCGTATGGACACCAGCGGCCTCGACACCGCGTACTGGATCAAA
AACCTCCGCGAACGCGTACGCTTCGCCGACGCCGTACGAGCACTCCTGCACGACGGCCAC
CGCGTATTCATCGAGGCCAGCACCCACCCCGTCCTCACCATCGGCATGCAGGAGAGCATC
GAGGAGGCGAGGGTCGACGCGGTCACCATCCCGACCCTGCGGCGCGACCACGGTGGCCCG
GACCAGCTGGCGCGGTCCGTGGCGCGGGCGTTCACCGCCGGGTTGTCGGTCGACTGGACC
CGCTGGTTTCCCACCGACCCCGCACCCCGCACCATCGACCTGCCCACATACGCCTTCCAG
CGTGAGCGGTACTGGCTGGACGGAGAGGGCGGACCGGGTGGCGATCCGGCCGGTCTGGGG
CTGACGGCCGCCGGCCATCCGCTGCTCGGCGCGGCCGTGGAACTCGCCGACGGCACCACC
CACGTGCTCACCGGCCGGATCTCCGCACGGTCCCACCCGTGGCTCGCCGAGCATGTGGTG
GCGGGTGCGGTGCTGGCGCCGGGTGCGGTGCTGGTGGAGTGGGCGCTGCGGGCGGCCGAC
GCGGTCGGCTGCGGCGGTGTGGAGGAGCTCGCGCTCCAGGTTCCGCTGGCGCTGCCCGAG
TCCGGTGGGCTGCGGGTGCAGGTGGTGGTGGGTGCCGCGGCGGAGGACGGGCGGCGCGAG
GTGCGGATGTACTCCAGCCCCGACCACCCCAACTCCCTTGATCCGGGCCTTGATTCCGGC
GCGGACACGGGGTGGGTCTGCCATGCGGTGGGCGTGCTCGCGCCCGCGGCCGAGGCCCCC
GCACCGGTGGAGGGCCTGGGCGGGGCCTGGCCGCCACCGGGCGCGGAGCCGCTGGACGTC
GGCGCCTTCTATGAGCAGGTGCTGGCGGCGGGGTACGGATACGGCCCGGCATACCAGGGG
CTGAAGGCCGCGTGGCGCCAGGGCGGGGATGTCCTCGCCGAGGTGGCGCTGCCCGATGCG
GCGGGCGGCCGGGACGGATACGGCATCCATCCGGCGCTGCTGGACGCGGCCTTGCATCCG
TCGCTGCTGATGGACCGGCCCGAGGGCCAGGAGGACGACGGCCAGGTGTGGTTGCCGTTC
GCGTGGAACGGCGTGTCCCTGTGGGCCACCGAGGCGGCCACGGTACGGGTCCGGCTCTCG
CGGGACGAGGAGCGGCAGTCGCTGCGGCTGACAGTGGCCGACACGGTGGGCGCCCCGGTG
CTGACGGTCGATTCGCTGGTGACCCGTCCGGCCGCGGCGGACCAGCTGTGCTCGGCCGCG
GCCGCGCGCGCCGTGGACGGACTGTTCACCCTGGACTGGATGGCGGTGCCCGCTCCGGCC
ACCGCGCCGTCCCCGGCGTCGGTGGTCGGCGAAGGTAGTTGGGTGGTGCTGGGCGAGGAC
CGGCTGGGGCTGGCCGAGCCGGTCGCGGCCGGGTTGGGCGCCGATGCCACGGTGAGCCAT
CCGGACCTGGAGGCGCTGATCGCGGCTGTCGACGCGGGCGAGCCCGCCCCCGCCGTCGTC
CTGACCCATCCGTACACCGCTGAGGGCGGCCACGGGGACGGGCTGGGGGCGGTCGAGGAG
CTGTTGGGGCTGGTGCGGTCCTGGCTGCGGCGACCGGCGCTGGCCGACGCACGGCTGGTG
GTCGTCACGCGGGGCGCGGTGGCGGCGGGCCGTGCGGGCGACGCCACCGATGACGCCGAT
GACACGGCGCTGGATCCGTCCGGTGTGGATCCGTCCGGTGCCGGGGCGTGGGGGCTGATC
CGCAGCGCCCAGTCGGAGAACCCGGGCCGGTTTGTACTGCTCGACCTCGATGAAGGCCAC
GAGGGCCGTGCGGGCCTCGTGGAGGCCGTGGTGCGCGCGGTCGAGTCGCATGAGCCGCAG
ATGGCGGTGCGGGACGGGCGGGTGCTGGTGCCCCGGATGGTCCGCGCGATGGCCGCGCCG
GGCCCGACCACCGACGACCGGGGCGAGTCCACCACCGGACTGGACGAGAACACCACCGGA
CTGGACGGGGGCGCCACCGGGCTGGACACGGATGGCACCGTGCTCATCACGGGTGGCACC
GGCACGCTGGGCACCCTGGTCGCCGAGCATCTGGTGCGCGCCTGGGGTGTCCGGCATCTG
CTGTTGGTGAGCCGAAGCGGTCCGGAGGCCGCCGGGGCCGGTGAACTCACGGCCGCCCTT
ACGGAGTTGGGCGCTGAGGTACGGATCGCCGCGGTCGATGTGACCGACCCGGACGCGGTG
GCCGCTCTGGTGGCCGGGGTCGACTCCGCGCATCCGCTGACCGGTGTCGTCCACGCGGCC
GGGGTGCTGGACGACGGCGTTCTCACCACCCAGACCCCGGAGCGGCTGGCGCGGGTGTGG
CGTCCGAAGGCCACGGCCGCGGCCCATCTGCACGCGGCGACCGCGGAGCTGCCGCTGTCC
CTGTTCGTGATGTTCTCCTCCACGGCGGGCACGCTGGGCGCCTCGGGACAGTCCAACTAC
GCTGCGGCGAACGCCTATTGCGACGCCCTGGCCGCCCGGCGCCACGCACTCGGCCTGCCG
GGGCTTTCGGTGGCGTGGGGCCTGTGGGCGGACGCCAGCGGGATGACCGGGCATCTGGGG
GAGGCGGACCGGGCCAGGATGACCCGGTCGGGCATCGGGGCGATGTCCAGCGAGCGGGCG
CTGGGGCTGCTGGACGCCGCCATGCGCCATGGCCACCACCATCTGATCGCGATCGATCTG
GACGTACGGGCCCTGACGGGCCGGCCGGCGCTCACCCTGCCCGCTCCGCTGCGGGCGCTC
GCCGGTGGCGGTACGGCCCGGCGCACCGCGGCCACCGCCCGGCCGCACACCGACTGGGCC
GGTCATCTGGCCGCGCTGCCGCTGGAGGAGCAGCGCCGTACGCTGCTCAATCTGGTCCTC
AGCCATGCCGCGGCGGCGCTTGGCCACTCCGATCCGGGGCGGATCCAGACCGAGCGCGGA
TTCCTGGAGCTCGGTTTCGACTCGCTGACCGCGATCGAACTGCGGAACAGGCTGACCGCG
GTGACCGGGCTGCGGCTGGCCACCACCCTGATCTTCGACCATCCGAATCCGGCCGCGCTC
GCCGCCCATCTGCATTCGGAATTGGCGCCCGAGGATGCGGATCCGCTCGCCCCGATGCTC
GGTGAAATCGACAGGATGGAAAGCGCGCTGCTTTCCGTGGCCCAGGACGAGACGGCCCAT
GAAGCGCTGCTCGAGCGCCTTCGGACCACGCTGTCGAAACTGGGTGCCCTGCACGGCGGC
GGGACGGAGGAGGGCCCCGCTGCGGGTCGTATTCAGGACGCCACGGCCGACGAGATCTTC
CAGTTCATCGACGAGGACCTTGGCCGAAGTGAAAGCAATGGAGAGCATGCTCATGGGGGC
ACACGGTGA
[14] KS34..410
[14] AT575..896
[14] methylmalonyl-CoA769..773
[14] DH946..1121
[14] KR1492..1672
[14] ACP1775..1845
[14] KS100..1230
[14] AT1723..2688
[14] methylmalonyl-CoA2305..2319
[14] DH2836..3363
[14] KR4474..5016
[14] ACP5323..5535

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [572-698]  3.70000000000004e-70 G3DSA:3.40.366.10 [769-885]  3.70000000000004e-70 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
 [1803-1818]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1775-1845]  PS50075
PS50075   ACP_DOMAIN
 [1779-1844]  9.60000000000001e-11 PF00550
PF00550   PP-binding
 [1773-1849]  6.90000000000001e-20 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1767-1862]  4.00000300869883e-23 SSF47336
SSF47336   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
 [1492-1671]  5.10000000000004e-60 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [34-284]  5.30000000000001e-96 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [292-410]  1.6e-48 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [575-896]  2.20000000000002e-99 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [4-34]  9.39999974783881e-08 SSF101173
SSF101173   Polyketide_synth_docking
 [1-27]  2e-10 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [573-878]  3.29999077503323e-64 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [700-768]  2.79999889404114e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [33-295]  4.59999999999996e-91 G3DSA:3.40.47.10 [296-462]  3.99999999999998e-66 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [26-399]  4.50001022902825e-105 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1491-1671]  4.70000000000003e-34 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [577-877]  1.09999184471405e-115 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1776-1848]  1.79999754022375e-32 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
 [946-1121]  2.1000026783403e-77 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [36-462]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1492-1672]  1.3000049540733e-58 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit