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CDS information : Oxzol_00100


close this sectionLocation

Organism
StrainJA3453
Entry nameOxazolomycin
Contig
Start / Stop / Direction17,620 / 16,520 / - [in whole cluster]
17,620 / 16,520 / - [in contig]
Locationcomplement(16520..17620) [in whole cluster]
complement(16520..17620) [in contig]
TypeCDS
Length1,101 bp (366 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)acyl ACP dehydrogenase
Gene
Gene (GenBank)ozmD
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
Reference
ACC
PmId
[16707707] Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from Streptomyces albus JA3453. (J Bacteriol. , 2006)
[20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010)
Related Reference
ACC
Q8KUG3
NITE
Ansam_00170
PmId
[11960423] Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis. (J Am Chem Soc. , 2002)
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
ACC
Q9KIE5
NITE
Asco_00090
PmId
[14623185] Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis. (J Mol Biol. , 2003)
[15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004)

close this sectionSequence

selected fasta
>putative oxidoreductase [acyl ACP dehydrogenase]
MSDELAALVSAEAGDRAAEWDLAGAIPPDVLRGLGAKGALCAEVPAEYGGRGLTGRANGA
LTAHAGALCSSLRSVMTSQGMAAWTIQRFGTRRQRTEELTRLTSGGLAAVAFSEPQAGSD
LSAIATTIVDDGDEVVLNGHKVWSTAAAYADVVIVFGRYQDGAAAVVVPTDAPGVTVRPV
AQPLGCRAAGHADVDLDDVRLPAGRVLSGASQSPGLLTTIALTYGRLSVAWGCVGIIRGC
LRAAATHARHREQFGKPLAQHQLVARHLSELLVAEQTATRVCEHASDCWDSGSPELSGAA
VLAKHVSAGRAAHAAATAVQVLASAGSVDGEPVARAYRDAKLMQIIEGSDEICQLILAER
ALEGVA
selected fasta
>putative oxidoreductase [acyl ACP dehydrogenase]
GTGAGTGACGAACTCGCGGCCCTGGTGAGCGCGGAGGCGGGTGACCGGGCCGCGGAGTGG
GACCTGGCCGGGGCCATTCCGCCGGACGTGCTGCGCGGGCTCGGCGCCAAGGGCGCGCTG
TGCGCCGAGGTGCCCGCCGAGTACGGCGGGCGCGGCCTGACGGGCCGGGCCAACGGTGCG
CTGACCGCGCACGCGGGGGCGTTGTGCAGCTCGCTGCGGTCGGTGATGACCTCGCAGGGC
ATGGCCGCCTGGACCATCCAGCGCTTCGGCACCCGCCGCCAGCGGACCGAAGAACTCACC
CGGCTCACCTCGGGCGGCCTCGCCGCCGTGGCGTTCAGCGAACCGCAGGCCGGCAGCGAC
CTCTCGGCGATCGCCACCACCATCGTGGACGACGGCGACGAGGTGGTCCTGAACGGTCAC
AAGGTCTGGTCGACCGCCGCCGCCTACGCCGACGTCGTCATCGTCTTCGGCCGCTACCAG
GACGGCGCGGCGGCCGTGGTGGTCCCCACCGACGCCCCGGGCGTCACGGTGCGCCCCGTC
GCCCAGCCGCTCGGCTGCCGCGCCGCGGGCCACGCCGACGTGGACCTCGACGACGTGCGC
CTGCCCGCCGGTCGCGTGCTGTCCGGCGCGAGCCAGTCGCCCGGGCTGCTGACCACCATC
GCCCTGACCTACGGCCGCCTGTCGGTGGCCTGGGGGTGTGTCGGCATCATCCGCGGCTGT
CTGCGGGCCGCCGCCACCCATGCCCGCCACCGCGAACAGTTCGGCAAGCCGCTGGCACAG
CACCAGCTCGTCGCCCGGCACCTGTCGGAGCTGCTGGTCGCCGAGCAGACCGCGACACGG
GTGTGCGAACACGCCTCGGACTGCTGGGACAGCGGCTCGCCCGAGCTGTCCGGCGCGGCG
GTGCTCGCCAAGCACGTCTCCGCAGGCCGTGCGGCACACGCCGCCGCCACCGCGGTGCAG
GTACTCGCGTCGGCGGGCTCGGTGGACGGCGAGCCGGTGGCCCGCGCCTACCGGGACGCC
AAGTTGATGCAGATCATCGAGGGAAGCGACGAGATCTGCCAGTTGATCCTCGCGGAACGC
GCGCTGGAGGGTGTGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain)
 [221-362]  4.80000000000001e-31 PF00441
PF00441   Acyl-CoA_dh_1
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain)
 [109-199]  1.8e-29 G3DSA:2.40.110.10
G3DSA:2.40.110.10   Acyl_CoA_DH/ox_M
 [109-158]  2.4e-16 PF02770
PF02770   Acyl-CoA_dh_M
IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain)
 [3-105]  9.20000000000002e-14 PF02771
PF02771   Acyl-CoA_dh_N
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain)
 [221-363]  1.29999924468179e-31 SSF47203
SSF47203   AcylCoADH_C_like
 [200-363]  2.1e-37 G3DSA:1.20.140.10
G3DSA:1.20.140.10   AcylCoA_DH_1/2_C
IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain)
 [4-212]  1.40000506907309e-52 SSF56645
SSF56645   AcylCoA_dehyd_NM
IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain)
 [4-105]  1.3e-22 G3DSA:1.10.540.10
G3DSA:1.10.540.10   AcylCoA_DH/ox_N
SignalP No significant hit
TMHMM No significant hit