close all open/close all
first first   Pikro_00020 Pikro_00020

CDS information : Pikro_00010


close this sectionLocation

Organism
StrainATCC 15439
Entry namePikromycin
Contig
Start / Stop / Direction154 / 1,122 / + [in whole cluster]
154 / 1,122 / + [in contig]
Location154..1122 [in whole cluster]
154..1122 [in contig]
TypeCDS
Length969 bp (322 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Product23S rRNA adenine N-6-methyltransferase
Product (GenBank)rRNA methyltransferase PikR2
Gene
Gene (GenBank)pikR2
EC number2.1.1.-
Keyword
Note
Note (GenBank)
  • mls type resistance protein
Reference
ACC
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[26438831] Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides. (Proc Natl Acad Sci U S A. , 2015)
Related Reference
ACC
Q9R622
PmId
[1328813] Molecular cloning and characterization of two lincomycin-resistance genes, lmrA and lmrB, from Streptomyces lincolnensis 78-11. (Mol Microbiol. , 1992)
ACC
P07287
PmId
[7961464] Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase. (J Bacteriol. , 1994)
[7793855] Erythromycin resistance by ribosome modification. (Antimicrob Agents Chemother. , 1995)

close this sectionSequence

selected fasta
>23S rRNA adenine N-6-methyltransferase [rRNA methyltransferase PikR2]
MAFSPQGGRHELGQNFLVDRSVIDEIDGLVARTKGPILEIGPGDGALTLPLSRHGRPITA
VELDGRRAQRLGARTPGHVTVVHHDFLQYPLPRNPHVVVGNVPFHLTTAIMRRLLDAQHW
HTAVLLVQWEVARRRAGVGGSTLLTAGWAPWYEFDLHSRVPARAFRPMPGVDGGVLAIRR
RSAPLVGQVKTYQDFVRQVFTGKGNGLKEILRRTGRISQRDLATWLRRNEISPHALPKDL
KPGQWASLWELTGGTADGSFDGTAGGGAAGSHGAARVGAGHPGGRVSASRRGVPQARRGR
GHAVRSSTGTEPRWGRGRAESA
selected fasta
>23S rRNA adenine N-6-methyltransferase [rRNA methyltransferase PikR2]
ATGGCATTTTCCCCGCAGGGCGGCCGACACGAGCTCGGTCAGAACTTCCTCGTCGACCGG
TCAGTGATCGACGAGATCGACGGCCTGGTGGCCAGGACCAAGGGTCCGATACTGGAGATC
GGTCCGGGTGACGGCGCCCTGACCCTGCCGCTGAGCAGGCACGGCAGGCCGATCACCGCC
GTCGAGCTCGACGGCCGGCGCGCGCAGCGCCTCGGTGCCCGCACCCCCGGTCATGTGACC
GTGGTGCACCACGACTTCCTGCAGTACCCGCTGCCGCGCAACCCGCATGTGGTCGTCGGC
AACGTCCCCTTCCATCTGACGACGGCGATCATGCGGCGGCTGCTCGACGCCCAGCACTGG
CACACCGCCGTCCTCCTCGTCCAGTGGGAGGTCGCCCGGCGCCGGGCCGGCGTCGGCGGG
TCGACGCTGCTGACGGCCGGCTGGGCGCCCTGGTACGAGTTCGACCTGCACTCCCGGGTC
CCCGCGCGGGCCTTCCGTCCGATGCCGGGCGTGGACGGAGGAGTACTGGCCATCCGGCGG
CGGTCCGCGCCGCTCGTGGGCCAGGTGAAGACGTACCAGGACTTCGTACGCCAGGTGTTC
ACCGGCAAGGGGAACGGGCTGAAGGAGATCCTGCGGCGGACCGGGCGGATCTCGCAGCGG
GACCTGGCGACCTGGCTGCGGAGGAACGAGATCTCGCCGCACGCGCTGCCCAAGGACCTG
AAGCCCGGGCAGTGGGCGTCGCTGTGGGAGCTGACCGGCGGCACGGCCGACGGATCCTTC
GACGGTACGGCGGGCGGTGGCGCGGCCGGATCGCACGGGGCGGCTCGGGTCGGGGCCGGT
CACCCGGGCGGCCGGGTGTCCGCGAGCCGGCGGGGCGTGCCGCAGGCGCGGCGCGGCCGG
GGGCATGCGGTACGGAGCTCCACGGGGACCGAGCCGAGGTGGGGCAGGGGGCGGGCGGAG
AGCGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001737 Ribosomal RNA adenine methylase transferase (Family)
 [8-250]  3.09999999999998e-59 PF00398
PF00398   RrnaAD
IPR020596 Ribosomal RNA adenine methylase transferase, conserved site (Conserved_site)
 [37-64]  PS01131
PS01131   RRNA_A_DIMETH
IPR020598 Ribosomal RNA adenine methylase transferase, N-terminal (Domain)
 [22-182]  1.3000049540733e-69 SM00650
SM00650   rADc
SignalP No significant hit
TMHMM No significant hit
first first   Pikro_00020 Pikro_00020