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CDS information : Pimar_00050


close this sectionLocation

Organism
StrainATCC 27448 (=NBRC 13367)
Entry namePimaricin
Contig
Start / Stop / Direction16,480 / 10,406 / - [in whole cluster]
9,736 / 3,662 / - [in contig]
Locationcomplement(10406..16480) [in whole cluster]
complement(3662..9736) [in contig]
TypeCDS
Length6,075 bp (2,024 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)PimS4 protein
Gene
Gene (GenBank)pimS4
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000)

close this sectionPKS/NRPS Module

12 malonyl-CoA
KS38..410
AT551..856
DH904..1061
KR1370..1550
ACP1651..1721
TE1809..2018

close this sectionSequence

selected fasta
>polyketide synthase [PimS4 protein]
MPNDKPNDKKLVDYLKWVTNDLHQTRQRLREVESAKQEPIAIVGMSCRLPGGVRSPEEFW
QLLKDGRDGISTFPTDRGWDLDLLAGDGSGSSATAEGGFIDAASFDAGFFGISPREAVTM
DPQQRILLESSWEALERAGIDPVSLRGTETGVFVGTSGIDYINTVMNSREDIEGHGSTGL
IASILSGRVSYTFGLEGPAVTVDTACSSSLVSLHLAAQALRVGECSLALAGGVTAISTPM
SFVGFSRQGGLAPDGRCKAFSDTADGTAWSEGVGVLVVERLSDARRNGHPVLAVVRGSAV
NQDGASNGLTAPSGPSQQRVIRQALAAAGLSASDVDVVEAHGTGTALGDPIEAQALLATY
GRDRDPERPLLLGSVKSNLGHTQAAAGAAGLIKMILAMRHGVLPRTLHVEAPSSHVDWSS
GALQLLTEPQEWPKADRPWRAGVSSFAISGTNAHIILEQPPAAEEPEDEEGPHTSPSAVP
WLVSAKSEEALTAQLDRISALTDRSPLDLGLSLATTRAHLEHRAVLLSEGGETVEVARGH
AEHTAGKLAVLFSGQGAQRVGMGRELYGRFPVFAEALDGVLAHFDAGLRDVMFGEAEGLD
ETGFTQPALFAIEVALFRLSESLGVRPDFVAGHSIGEIAAAHVAGVFSLADACALVAARA
RLMQALPAGGAMVAVQAAESEVAARLVDGVSIAAVNGPEAVVIAGEEREVLRIAEEFTGL
GRKTRRLPVSHAFHSPLMEPMLEDFRRVAEGLSYEAPRIALVSNLTGELATEDLVRSAEY
WVRHVRETVRFADGVSALAAEGASVFLELGPDGVLTAMAQHTLDGTATTVPALRKDRSEE
TALLTALAQLHVAGVGVDWSGIFAGTGARRVDLPTYPFQHERYWPRPAVLSGDVVGAGLR
SAEHPLLGATLTLAESGEVVLTGRLSLQAYPWLTDHTMGGSVLFPATGFLELAMRAADQV
GCDRVDEFVLMTPLVLAEESGTQVQVLVGPADEAGTRTISIHARPDGDPDQPWMQHAGGT
LTSGERIVDFGSTVWPPQGAEAVDLEGFYDTTGYGPAFQGLRSVWQCEDGAYVEVALPDQ
VAEDAGAFGLHPVLLDAVLQSRRMAGIGAAGDTFLPFAWRGVSLHAGGASVLRARVIKTG
EDTLSLVAVDAEGAPVLSVEELVLRTQLTHTDAPLTARRGAERDALLSLEWVSGPEVPPA
GVRCVSLGADELGVGASVATLADLSGDEDFVVVPVSGVGGDVPAAAHELTARTLGLLQEW
SGREGTSASRLVFVTRNAVSADGGESVRDVAAAAVWGLVRSAQSESPGRIALVDLEADSG
DLAEVLAGLPGLLAAGDAQFVVREGAVRVGRLVESNEGAGVSPREWDADGTVLVTGGTGG
LGRELARHLVVERGVRHLLLVSRSGPEASGVGELRDELAAHGAEIAVRACDVADRAAVEA
VVGAVDAAHPLTAVIHTAGVLDDGVIASLTPERLSAVLRPKVDAAWNLHEATKDLDLAAF
VLYSSISGVLGSLGQGNYAAGNVFLDALAGHRAGLGLPAQSLAWGAWTQGGGMTATLSDA
DMQRMASYGTAPLSPERGLALFDAATAGSATHLVAVGRVSGPVRAQGPVPSMLRGLVKGA
RRTAVNTTGETAVSLADRLRAARPEERTQLMTDVVRTEAAAVLGHASSQNVDARREFYEL
GFDSLTSVELRNRLSTVTGLRLSATVVFDSKNPTDLAARLYADLAAQSALDGSASQAGFL
PVAAPETDSLERMFLDALDSGKVPEAQRMLSALGALRPSFENTAELEDLPLPATLAEGPG
TPRLICVSTPTANGGVHEYARLAASFRGERHVSALPLVGFATGERLPATAETAVRVIAES
ALRASDGNPFVLVGHSSAGAFAYLAAALLENTWGIRPEAVVLLDTLSLRHEQNESIDYAG
LMRRHFMVDEVSPVRMTNSRLSAMARWMGMLNQLEVRHTTVPVLIIRATKETFGIGTDTG
IYGEDHGSPVDVRSVDADHFSMVRDDAPETARIVREWLDSLGNA
selected fasta
>polyketide synthase [PimS4 protein]
ATGCCGAACGACAAGCCGAACGACAAAAAGCTCGTTGACTACCTCAAGTGGGTCACGAAC
GACTTGCACCAGACCCGCCAACGGCTTCGGGAAGTCGAGTCCGCCAAGCAGGAACCGATA
GCCATCGTGGGCATGTCCTGCCGACTACCGGGTGGCGTGCGGTCGCCGGAAGAATTCTGG
CAGTTGTTGAAGGACGGCCGGGACGGCATCTCGACCTTCCCCACGGACCGCGGCTGGGAC
CTCGACCTGCTCGCCGGCGACGGCAGTGGCAGCAGTGCCACCGCCGAAGGCGGCTTCATC
GACGCCGCCTCGTTCGACGCGGGCTTCTTCGGCATCTCGCCCCGCGAGGCCGTGACGATG
GACCCCCAGCAGCGCATCCTGCTGGAGTCCTCCTGGGAGGCCCTGGAGCGCGCCGGAATC
GACCCCGTGTCCCTGCGGGGCACCGAGACCGGTGTCTTCGTGGGCACGAGCGGTATCGAC
TACATCAACACCGTGATGAACTCCCGCGAGGACATCGAAGGCCACGGCAGCACCGGCCTC
ATCGCCAGCATCCTGTCCGGCCGGGTCTCGTACACCTTCGGCCTGGAGGGCCCGGCCGTC
ACCGTCGACACCGCATGCTCCTCCTCCCTGGTCTCACTGCACCTGGCGGCCCAGGCGCTG
CGTGTGGGGGAGTGCTCGCTGGCGCTGGCCGGAGGGGTGACGGCGATATCGACGCCGATG
AGCTTCGTCGGATTCAGCAGGCAGGGCGGGCTGGCGCCGGACGGCCGGTGCAAGGCGTTC
TCCGACACGGCCGACGGCACCGCATGGTCCGAGGGCGTGGGCGTTCTGGTCGTGGAGCGC
CTGTCCGACGCCCGCCGCAACGGGCATCCGGTCCTCGCGGTCGTACGGGGCTCGGCCGTC
AACCAGGACGGCGCCTCCAACGGCCTGACGGCCCCCAGCGGTCCCTCCCAGCAGCGGGTG
ATCCGGCAGGCGCTGGCCGCCGCCGGTCTGTCGGCCTCGGACGTGGACGTGGTCGAGGCC
CATGGCACCGGCACCGCGCTCGGTGATCCGATCGAGGCGCAGGCGCTGCTGGCCACGTAC
GGCCGGGACCGGGACCCCGAGCGGCCGCTGCTGCTGGGGTCGGTCAAGTCCAACCTCGGT
CACACGCAGGCGGCGGCCGGCGCGGCCGGGCTGATCAAGATGATCCTGGCGATGCGGCAC
GGCGTCCTGCCCAGGACGCTGCATGTGGAGGCGCCGTCGTCACATGTGGACTGGTCGTCC
GGTGCGCTGCAACTGCTGACGGAGCCTCAGGAGTGGCCCAAGGCCGACCGGCCGTGGCGG
GCCGGCGTGTCGTCCTTCGCCATCAGCGGCACCAACGCCCACATCATCCTTGAGCAGCCA
CCGGCCGCCGAGGAGCCCGAGGACGAGGAAGGGCCGCATACCTCGCCGTCGGCCGTGCCC
TGGCTGGTCTCCGCGAAGTCCGAGGAGGCACTGACCGCGCAGCTCGACCGGATCTCCGCG
CTGACCGACCGGTCGCCTCTCGACCTGGGCCTCTCACTGGCGACCACCCGGGCGCATCTG
GAGCACCGTGCGGTGCTGCTGTCCGAGGGCGGCGAAACCGTCGAGGTGGCACGCGGGCAC
GCCGAGCACACGGCCGGGAAGCTGGCGGTGCTGTTCTCGGGGCAGGGTGCTCAACGCGTC
GGGATGGGCCGGGAGTTGTACGGTCGGTTCCCGGTGTTCGCCGAGGCGTTGGACGGGGTG
CTGGCGCACTTCGATGCCGGGTTGCGGGATGTGATGTTCGGCGAGGCGGAGGGCCTGGAC
GAGACCGGGTTCACCCAGCCCGCGCTGTTCGCCATCGAGGTGGCGTTGTTCCGGCTGTCT
GAGTCTCTGGGTGTGCGGCCGGATTTCGTGGCCGGGCACTCGATCGGTGAGATCGCGGCG
GCGCATGTCGCCGGGGTGTTCTCGCTGGCGGATGCGTGTGCGTTGGTGGCGGCCCGTGCG
CGGCTGATGCAGGCCCTCCCGGCGGGCGGCGCGATGGTCGCCGTTCAGGCGGCAGAGAGC
GAGGTGGCTGCACGGCTGGTCGACGGGGTGTCGATCGCGGCGGTCAACGGCCCGGAGGCG
GTGGTGATCGCAGGGGAGGAGCGCGAAGTCCTGCGGATCGCCGAGGAGTTCACCGGCCTG
GGGCGTAAGACACGGCGCCTGCCGGTCAGCCACGCCTTCCACTCGCCGCTGATGGAGCCG
ATGCTGGAGGACTTCCGACGGGTCGCCGAGGGACTGTCGTACGAAGCCCCGCGGATCGCG
CTGGTGTCGAACCTCACCGGCGAACTCGCCACCGAGGACCTCGTCCGCTCGGCCGAGTAC
TGGGTCCGGCATGTGCGCGAGACCGTGCGCTTCGCCGACGGTGTGAGTGCCCTGGCCGCC
GAAGGGGCCTCCGTCTTCCTGGAGTTGGGCCCTGACGGTGTGCTGACGGCGATGGCCCAG
CACACCCTGGACGGCACCGCGACCACCGTACCGGCCCTGCGTAAGGACCGGTCCGAGGAG
ACCGCGCTGCTCACCGCCCTCGCCCAGCTGCATGTCGCGGGCGTCGGGGTGGACTGGTCC
GGGATCTTCGCCGGCACCGGTGCCCGCCGCGTCGACCTGCCCACCTACCCCTTCCAGCAC
GAGCGCTACTGGCCTCGTCCCGCCGTGCTCAGCGGCGATGTGGTCGGTGCGGGTCTGCGG
TCCGCCGAGCACCCGCTGCTGGGCGCGACGCTCACCCTCGCGGAGTCCGGTGAGGTGGTG
CTGACGGGCAGGCTGTCGCTTCAGGCATACCCGTGGCTGACCGACCACACCATGGGCGGC
AGCGTCCTCTTCCCGGCCACCGGCTTTCTGGAGCTGGCGATGCGCGCCGCCGATCAGGTC
GGCTGCGACCGGGTCGACGAGTTCGTCCTGATGACTCCGCTGGTGCTCGCGGAGGAGAGC
GGCACCCAGGTCCAGGTGCTGGTCGGACCCGCCGACGAGGCAGGCACCCGCACGATCTCC
ATCCATGCCCGCCCCGACGGCGACCCCGACCAGCCGTGGATGCAGCACGCGGGCGGCACG
CTGACCAGCGGTGAACGGATCGTGGACTTCGGGTCCACGGTCTGGCCGCCGCAGGGCGCC
GAGGCCGTCGACCTGGAGGGCTTCTACGACACGACGGGTTACGGCCCGGCCTTCCAGGGC
CTGCGTTCGGTGTGGCAGTGCGAGGACGGTGCCTACGTGGAGGTGGCGCTGCCGGACCAG
GTCGCCGAGGACGCCGGGGCCTTCGGGCTCCATCCGGTGCTGCTGGACGCGGTGCTCCAG
TCGCGCCGGATGGCCGGGATCGGCGCTGCGGGGGACACGTTCCTGCCCTTCGCCTGGCGC
GGTGTGTCGCTGCATGCGGGCGGCGCCTCGGTCCTGCGGGCCCGGGTGATCAAAACCGGC
GAGGACACGCTGTCTCTTGTGGCCGTCGACGCCGAAGGCGCCCCCGTCCTGTCCGTCGAG
GAACTCGTTCTGCGCACCCAACTCACCCATACAGACGCACCGTTGACGGCGCGACGTGGC
GCGGAGCGCGACGCACTGTTGTCGTTGGAGTGGGTGTCGGGTCCTGAGGTGCCGCCTGCC
GGGGTGCGGTGCGTGTCCCTGGGTGCGGATGAGCTGGGTGTGGGCGCATCGGTTGCCACT
CTGGCGGACCTCTCCGGCGATGAGGATTTCGTGGTGGTTCCCGTGTCCGGTGTGGGTGGT
GATGTGCCTGCTGCCGCGCACGAGTTGACGGCCCGGACGCTCGGTCTGCTTCAGGAGTGG
TCCGGCCGGGAGGGGACGAGCGCTTCGCGTTTGGTGTTCGTCACCCGTAACGCGGTCTCC
GCCGACGGAGGCGAGTCCGTACGCGATGTGGCCGCGGCTGCGGTGTGGGGTCTGGTGCGC
TCGGCGCAGTCGGAGAGTCCGGGGCGGATCGCGCTGGTGGACCTGGAGGCCGACTCCGGA
GACCTGGCGGAGGTGCTTGCGGGTCTGCCGGGTCTGTTGGCTGCCGGGGACGCTCAGTTC
GTAGTGCGCGAAGGCGCTGTGCGGGTGGGGCGGTTGGTCGAGTCGAACGAGGGCGCGGGC
GTGTCGCCGCGGGAGTGGGATGCGGACGGCACGGTGCTGGTCACCGGTGGTACCGGGGGT
CTGGGGCGTGAGTTGGCCCGGCATCTGGTGGTCGAGCGCGGTGTACGGCATCTGCTGCTG
GTCAGCCGGAGCGGACCCGAGGCATCCGGCGTAGGGGAGCTGCGCGACGAACTGGCCGCC
CACGGCGCCGAGATAGCGGTCCGTGCCTGCGATGTAGCGGACCGGGCGGCGGTGGAGGCC
GTCGTGGGTGCGGTGGATGCCGCGCATCCGCTGACCGCGGTGATCCACACGGCCGGTGTT
CTCGATGACGGTGTGATCGCCTCGCTGACGCCGGAGCGGCTGTCGGCCGTACTGCGCCCG
AAGGTGGATGCGGCCTGGAATCTGCACGAGGCCACCAAGGACCTCGACCTGGCCGCGTTC
GTCCTGTACTCGTCCATCTCCGGAGTGCTCGGTTCTCTGGGACAGGGCAACTACGCGGCG
GGCAACGTGTTCCTCGATGCGCTGGCCGGGCACCGGGCGGGCCTGGGGCTGCCCGCGCAG
TCCCTGGCCTGGGGCGCCTGGACCCAGGGTGGCGGTATGACCGCCACCCTCTCCGATGCG
GACATGCAGCGGATGGCCTCGTACGGGACCGCACCGCTGAGCCCCGAGCGCGGCCTGGCG
CTCTTCGACGCCGCTACCGCCGGCAGCGCGACCCACCTCGTCGCGGTCGGCCGCGTCTCC
GGACCGGTGCGGGCCCAGGGCCCCGTCCCGTCGATGCTGCGCGGCCTGGTCAAGGGCGCC
CGCCGCACGGCCGTGAACACGACGGGAGAGACGGCCGTGTCCCTCGCCGACCGGCTGCGG
GCCGCGCGCCCCGAGGAGCGCACGCAGCTGATGACCGATGTCGTACGGACCGAGGCCGCC
GCCGTCCTCGGACACGCCTCATCCCAGAACGTCGACGCCCGCAGGGAGTTCTACGAGCTG
GGCTTCGACTCCCTGACCTCCGTCGAACTGCGCAACCGGCTCTCCACCGTCACCGGACTA
CGGCTGTCGGCAACCGTGGTCTTCGACAGCAAGAACCCCACCGACCTCGCGGCCCGGCTC
TACGCCGACCTGGCCGCCCAGTCGGCCCTGGACGGCTCCGCGTCGCAAGCGGGCTTCCTC
CCGGTCGCCGCACCGGAGACCGACTCGCTGGAGCGGATGTTCCTCGACGCCCTGGACAGC
GGCAAGGTCCCGGAGGCCCAGCGCATGCTCTCCGCCCTCGGCGCCCTGCGTCCGAGCTTC
GAGAACACCGCCGAACTGGAGGATCTGCCGCTCCCGGCAACCCTCGCCGAGGGCCCCGGC
ACGCCGCGGCTGATCTGTGTGAGCACACCCACCGCCAACGGTGGCGTACACGAATACGCC
AGGCTCGCCGCGTCCTTCCGAGGTGAGCGGCACGTCAGCGCGCTGCCGCTGGTCGGCTTT
GCCACGGGGGAGCGTCTGCCGGCCACTGCGGAGACCGCGGTGCGGGTCATCGCGGAGAGC
GCGCTGCGGGCGAGCGACGGCAACCCGTTCGTGCTGGTCGGGCACTCGTCCGCCGGCGCC
TTCGCCTACCTGGCTGCGGCCCTGCTGGAGAACACCTGGGGCATCCGGCCCGAGGCCGTC
GTGCTGCTGGACACCCTCAGCCTCCGGCACGAGCAGAACGAGAGCATCGACTACGCCGGC
CTGATGCGACGCCACTTCATGGTCGACGAGGTCTCGCCGGTACGGATGACGAACTCCAGG
CTGTCGGCGATGGCGCGCTGGATGGGCATGCTGAACCAGCTCGAAGTGCGGCACACGACC
GTTCCCGTACTGATCATCCGTGCCACCAAGGAGACCTTCGGCATCGGGACCGACACCGGA
ATCTACGGCGAGGACCACGGCAGCCCGGTCGACGTCCGCAGCGTGGACGCGGACCACTTC
TCCATGGTCCGCGACGACGCCCCGGAGACCGCGCGGATCGTCCGGGAATGGCTCGACTCC
CTCGGCAACGCGTGA
[12] KS38..410
[12] AT551..856
[12] malonyl-CoA731..735
[12] DH904..1061
[12] KR1370..1550
[12] ACP1651..1721
[12] TE1809..2018
[12] KS112..1230
[12] AT1651..2568
[12] malonyl-CoA2191..2205
[12] DH2710..3183
[12] KR4108..4650
[12] ACP4951..5163
[12] TE5425..6054

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
 [1809-2018]  1.69999999999999e-17 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
 [541-666]  1.20000000000001e-80 G3DSA:3.40.366.10 [731-846]  1.20000000000001e-80 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
 [1679-1694]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1654-1720]  4.40000000000001e-10 PF00550
PF00550   PP-binding
 [1651-1724]  7.4e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1644-1758]  1.79999754022375e-18 SSF47336
SSF47336   ACP_like
 [1651-1721]  PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
 [1370-1549]  4.10000000000004e-64 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [38-284]  3.10000000000005e-93 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [292-410]  3.40000000000004e-49 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [551-856]  8.10000000000001e-68 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [6-31]  2.7e-14 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [546-848]  7.0999832511256e-73 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [668-730]  1.80000149287196e-15 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [40-296]  1.60000000000002e-92 G3DSA:3.40.47.10 [297-462]  1.10000000000001e-65 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [30-408]  5.50001754266469e-102 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1369-1556]  2.99999999999998e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [197-213]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [551-837]  2.39999798157263e-119 SM00827
SM00827   PKS_AT
IPR020802 Polyketide synthase, thioesterase domain (Domain)
 [1805-2018]  6.29998179434741e-102 SM00824
SM00824   PKS_TE
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1652-1724]  1.29999924468179e-28 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
 [904-1061]  4.20003460493095e-76 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [40-462]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1370-1550]  9.69997533354407e-65 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit