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CDS information : Pimar_00060


close this sectionLocation

Organism
StrainATCC 27448 (=NBRC 13367)
Entry namePimaricin
Contig
Start / Stop / Direction21,942 / 16,516 / - [in whole cluster]
15,198 / 9,772 / - [in contig]
Locationcomplement(16516..21942) [in whole cluster]
complement(9772..15198) [in contig]
TypeCDS
Length5,427 bp (1,808 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)PimS3 protein
Gene
Gene (GenBank)pimS3
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000)

close this sectionPKS/NRPS Module

11 malonyl-CoA
KS35..408
AT552..857
DH905..1062
KR1371..1551
ACP1652..1722

close this sectionSequence

selected fasta
>polyketide synthase [PimS3 protein]
MTTSSETVVAALRASLKEAENLRRQNRKLVAAATEPIAIVAMACRYPGGVQSPEDLWELL
TAGKDAIGAFPTDRGWDLDALRDAGVDRRGNAVSQEGGFLDGVGDFDADFFGISPRESVT
MDPQQRLLLETSWEAIERAGIDPTTLRGSRTGVFIGTNGQDYAYLLVRSLADATGDIGTG
IAASATSGRLSYTLGLEGPAVTVDTACSSSLVALHTASHALRAGECSLALVGGVNVMSSP
GSLMEFSRQGGLAADGRCKAFSDTADGTGWSEGVGVLVVERLSDARRNGHPVLAVVRGSA
VNQDGASNGFTAPSGPSQQRVIRQALAAAGLSASDVDVVEAHGTGTPLGDPIEAQALLAT
YGQDRDPERPLLLGSVKSNLGHTQAAAGAAGLIKMILAMRHGVLPKTLYADVPSSHVDWT
SGEVELLTEPQEWPKADRPWRAGVSSFGISGTNAHVIVEQAEPDEEPVAEPGLAPAVVPW
LVSAKTEEALAAQLARISSRRGTASPVDVGFSLASGRSAFEHRAVLLAQCPDGELSEAAR
GRAETSRPLAVLFSGQGAQRVGMGRELYVRFPVFAEALDEVLAHFDAGLRDVMFGDAEGL
DETGFTQPALFAIEVALFRLAESLGVRPDFVGGHSIGEIAAAHVAGVFSLTDACALVAAR
ARLMQALPAGGAMVAVQASESEVAARLVDGVSIAAVNGPEAVVLAGDETAVLRIAEELAA
GGRKTQRLPVSHAFHSPLMEPMLEDFRRVAEGLSYGAPQIPLVSNVTGELAVEELVCSPD
YWVRHVRETVRFADGVSALEAEGASAFLELGPDGVLTAMARHTLDETKVAVPVLRKDRPE
ETALLTALAHLHVAGIGVDWSGIFAGTGARRVDLPTYPFQHERYWPRPAVLSGDVAGAGL
RSAEHPLLGATLTLAESGEVVLTGRLSLQTHPWLTDHAIGGNVFFPGTGFLELAMHAADQ
AGCDRVEDLVLMTPLVLAEENGTQVQVLVGPADEAGTRTISIHARPEDDPDQPWMQHAGG
TLTSGERVVDFGSTVWPPQDAVAVDLEGFYEGTGYGPAFQGLRSVWSCGDGAYAEVALPD
HVAEDAGAFGLHPALLDAVLQSHRIAGVGSADDQFLPFAWRGVSLHAGGASVLRVRVVKT
GDDSMSIAAVDAEGAPVLSAEAMVMRAYRDPGTGQAAARHGAERDELLSLEWVSGPEVPP
AGVRCVSLGADELGVGASVASLADLSGDEDFVVLPVSGVGGDVPAAAHELTARTLGLLQE
WSGREGSSGSRLVFVTRNAVSADEGESVRDVAAAAVWGLVRSAQSESPGRIALVDLEADS
GDLAEVLAGLPGLLAAGDAQFVVREGAVRVGRLVESNEGAGVSPREWDADGTVLVTGGTG
GLGRELARHLVVERGVRHLLLVSRSGPEAPGVSGLSDELAAHGAEVTVRACDVADRAAVH
ALVAAVPAEHPLTAVIHTAGVLDDGLVTSLTPERLSTVLRPKVDAAWNLHEATKDLDLAA
FVLYSSISGVLGAPGQANYAAGNVFLDALAWHRMGQGLPAQSLAWGAWTQDGGMTATLSD
TDMQRMASYGTAPLSRQRGLALFDAACARSAAHLVAVGRVGGPVRASAPVPSVLRGLVKG
ARRTAVNTTGETAVSLADRLRAARPEERPRLMTDLVRTEAAAVLGHASAKNVDARREFNE
LGFDSLTSVELRNRLSALTGLRLSAALVFDYPNPTALADHFVSELVEEAATGPALLAELE
RLDSALAAGAPDARTRAAVTARLAQILDDWRDAPAEEDGAEVAERLEAASTDEIFAFIDN
ELGRHSDR
selected fasta
>polyketide synthase [PimS3 protein]
ATGACCACATCCAGTGAAACCGTCGTCGCAGCCCTCCGCGCCTCGCTGAAGGAAGCCGAG
AACCTGCGCCGCCAGAACCGCAAGCTCGTCGCCGCGGCCACGGAGCCGATCGCGATCGTG
GCGATGGCCTGCCGCTACCCCGGCGGTGTCCAGTCGCCCGAGGACCTGTGGGAGCTGCTG
ACCGCGGGCAAGGACGCCATCGGCGCGTTCCCCACGGACCGCGGCTGGGACCTGGACGCC
CTGCGGGACGCCGGGGTCGACCGGCGCGGCAACGCCGTCAGCCAGGAAGGGGGCTTCCTC
GACGGCGTGGGCGACTTCGACGCGGACTTCTTCGGGATCTCACCTCGCGAATCCGTCACC
ATGGACCCGCAGCAGCGCCTCCTGCTGGAGACCTCCTGGGAGGCCATCGAACGCGCCGGC
ATCGACCCGACCACCCTGCGCGGCAGCCGCACCGGCGTGTTCATCGGCACCAACGGCCAG
GACTACGCCTACCTGCTCGTACGCTCCCTGGCCGACGCCACCGGCGACATCGGCACCGGA
ATCGCCGCCAGCGCCACCTCCGGGAGGCTGTCGTACACGCTGGGCCTGGAGGGCCCGGCG
GTCACCGTCGACACGGCGTGCTCGTCCTCGCTCGTGGCCCTGCACACGGCGAGCCACGCC
CTGCGGGCCGGTGAGTGCTCGCTGGCCCTGGTCGGCGGGGTGAACGTCATGTCGTCCCCC
GGTTCCCTGATGGAGTTCAGCAGGCAGGGCGGGCTCGCGGCGGACGGCCGGTGCAAGGCG
TTCTCCGACACGGCCGACGGCACCGGGTGGTCGGAGGGCGTGGGCGTTCTGGTCGTGGAG
CGCCTGTCCGACGCCCGCCGCAACGGGCATCCCGTCCTCGCGGTCGTACGGGGCTCGGCC
GTCAACCAGGACGGCGCCTCCAACGGCTTCACCGCGCCCAGCGGTCCCTCCCAGCAGCGG
GTGATCCGGCAGGCGCTGGCCGCCGCCGGTCTGTCGGCCTCGGACGTGGACGTGGTCGAG
GCCCACGGCACCGGCACCCCGCTCGGTGATCCGATCGAGGCGCAGGCGCTGCTGGCCACG
TACGGTCAGGACCGCGACCCCGAGCGGCCGCTGCTGCTGGGGTCGGTCAAGTCCAACCTC
GGCCATACCCAGGCGGCGGCCGGCGCGGCAGGGCTGATCAAGATGATCCTGGCGATGCGG
CACGGTGTCCTGCCCAAGACGCTGTACGCCGATGTGCCGTCCTCCCACGTGGACTGGACG
TCGGGGGAGGTGGAGCTGCTGACGGAGCCTCAGGAGTGGCCCAAGGCCGACCGCCCGTGG
CGGGCCGGCGTGTCCTCGTTCGGCATCAGCGGCACCAACGCCCATGTGATCGTGGAGCAG
GCGGAACCGGACGAAGAGCCGGTGGCGGAACCGGGGTTGGCGCCCGCGGTGGTGCCATGG
CTGGTGTCCGCCAAGACCGAGGAGGCGCTGGCGGCACAGCTCGCGCGGATCTCCTCGCGC
AGGGGCACGGCGTCACCGGTGGACGTGGGATTCTCGCTCGCCTCGGGCCGTTCGGCGTTC
GAGCACCGCGCGGTGCTGCTGGCCCAGTGCCCCGACGGCGAGCTGTCCGAGGCGGCCCGG
GGCCGCGCGGAGACGAGCCGCCCGCTGGCGGTGCTGTTCTCGGGGCAGGGTGCTCAACGG
GTGGGTATGGGCCGGGAGTTGTATGTCCGGTTCCCGGTGTTCGCCGAGGCGTTGGACGAG
GTGCTGGCGCACTTCGATGCCGGGTTGCGGGACGTGATGTTCGGTGATGCGGAGGGGTTG
GACGAGACCGGGTTCACGCAGCCCGCGCTGTTTGCCATTGAGGTGGCGTTGTTCCGGCTG
GCCGAGTCTCTGGGTGTGCGGCCGGATTTCGTGGGCGGGCACTCGATCGGTGAGATCGCG
GCGGCGCATGTCGCCGGGGTGTTCTCGCTGACGGATGCGTGTGCGTTGGTGGCGGCTCGG
GCGCGGCTGATGCAGGCCCTCCCGGCGGGCGGTGCCATGGTCGCCGTACAGGCGTCGGAG
AGCGAGGTGGCCGCACGGCTGGTCGACGGGGTGTCGATCGCGGCGGTCAACGGCCCGGAG
GCGGTGGTGCTCGCCGGGGACGAGACCGCGGTGCTCCGTATCGCGGAGGAGCTGGCGGCA
GGGGGACGCAAGACGCAGCGCCTGCCGGTCAGCCACGCCTTCCACTCGCCGCTGATGGAG
CCGATGCTGGAGGACTTCCGGCGGGTGGCCGAGGGGCTGTCGTACGGGGCTCCGCAGATC
CCGCTCGTCTCGAACGTGACCGGGGAGCTCGCGGTCGAGGAGCTGGTGTGCTCGCCGGAC
TATTGGGTGCGGCATGTACGGGAGACGGTGCGTTTCGCCGACGGTGTGAGCGCCCTGGAG
GCCGAAGGGGCCAGCGCCTTCCTGGAGTTGGGCCCGGACGGGGTACTGACCGCGATGGCC
CGGCACACTCTGGACGAGACGAAGGTGGCTGTCCCCGTATTGCGCAAGGACCGGCCCGAG
GAGACCGCGCTGCTCACCGCCCTCGCCCACCTGCATGTCGCGGGCATCGGGGTGGACTGG
TCCGGGATCTTCGCCGGCACCGGTGCCCGCCGCGTCGACCTGCCCACCTACCCCTTCCAG
CACGAGCGCTACTGGCCTCGTCCTGCCGTGCTCAGCGGAGATGTGGCCGGTGCGGGTCTG
CGGTCCGCGGAGCACCCGCTGCTGGGTGCGACGCTCACCCTCGCGGAGTCCGGTGAGGTG
GTGCTGACGGGCAGGCTGTCGCTGCAAACGCATCCGTGGCTGACCGACCACGCCATCGGC
GGCAACGTCTTCTTCCCGGGAACCGGCTTTCTGGAGCTGGCGATGCACGCCGCCGACCAG
GCCGGCTGCGACCGCGTAGAGGACCTCGTCCTGATGACTCCGCTGGTGCTCGCGGAGGAG
AACGGCACCCAGGTCCAGGTGCTGGTCGGACCCGCCGACGAGGCAGGCACCCGCACGATC
TCCATCCATGCCCGCCCGGAAGACGACCCCGACCAGCCGTGGATGCAGCACGCGGGCGGC
ACGCTGACCAGCGGCGAGCGCGTCGTGGACTTCGGTTCCACCGTGTGGCCGCCGCAGGAC
GCGGTGGCCGTCGACCTGGAGGGCTTCTACGAAGGGACCGGTTACGGCCCCGCGTTCCAG
GGCCTGCGTTCGGTGTGGAGCTGCGGGGACGGTGCCTACGCGGAAGTGGCCCTGCCGGAC
CACGTGGCCGAGGACGCCGGGGCCTTCGGGCTCCATCCGGCGCTGCTGGACGCGGTGTTG
CAGTCGCACCGCATCGCCGGGGTCGGCAGCGCGGACGACCAGTTCCTGCCCTTCGCCTGG
CGAGGCGTCTCGCTGCATGCGGGCGGCGCCTCGGTCCTGCGGGTCCGGGTCGTGAAGACC
GGCGACGACTCGATGTCCATCGCGGCCGTCGACGCCGAAGGCGCACCCGTACTGTCCGCC
GAGGCGATGGTGATGCGGGCCTACCGCGACCCCGGCACCGGCCAGGCGGCGGCACGGCAC
GGCGCGGAGCGCGATGAGCTGTTGTCGTTGGAGTGGGTGTCGGGTCCTGAGGTGCCGCCG
GCCGGGGTGCGGTGCGTGTCCCTGGGCGCGGATGAACTGGGTGTGGGCGCGTCGGTCGCC
TCGTTGGCGGACCTCTCCGGGGACGAGGATTTCGTGGTGCTTCCCGTGTCCGGTGTGGGT
GGTGATGTGCCTGCTGCCGCGCACGAGTTGACGGCCCGGACGCTCGGTCTGCTTCAGGAG
TGGTCCGGCCGCGAGGGCTCAAGTGGCTCGCGTTTGGTGTTCGTCACCCGTAACGCGGTC
TCCGCCGACGAAGGCGAGTCCGTACGCGATGTGGCCGCGGCTGCGGTGTGGGGTCTGGTG
CGCTCGGCGCAGTCGGAGAGTCCGGGGCGGATCGCGCTGGTGGACCTGGAGGCCGACTCC
GGCGATCTCGCGGAGGTGCTTGCGGGTCTGCCGGGTCTGTTGGCTGCCGGGGACGCTCAG
TTCGTAGTGCGCGAAGGTGCTGTGCGGGTGGGGCGGTTGGTCGAGTCGAACGAGGGCGCG
GGCGTGTCGCCGCGGGAGTGGGATGCGGACGGCACGGTGCTGGTCACCGGTGGTACCGGG
GGTCTGGGGCGTGAGTTGGCCCGGCATCTGGTGGTCGAGCGCGGTGTACGGCATCTGCTG
CTGGTCAGCCGGAGCGGACCCGAGGCGCCGGGCGTCTCCGGACTGAGCGACGAACTGGCC
GCCCACGGCGCGGAGGTCACCGTGCGCGCCTGCGACGTGGCCGACCGGGCGGCGGTGCAC
GCTCTCGTCGCTGCCGTACCGGCCGAACACCCGTTGACCGCCGTGATCCACACGGCCGGT
GTCCTCGATGACGGGCTGGTCACCTCGCTGACACCGGAGCGGCTGTCGACCGTACTGCGC
CCGAAGGTGGATGCGGCCTGGAATCTGCACGAGGCCACCAAGGACCTCGACCTGGCCGCG
TTCGTGCTGTACTCGTCCATCTCCGGGGTGCTCGGTGCTCCCGGACAGGCCAACTACGCG
GCAGGCAACGTGTTCCTCGATGCGCTCGCCTGGCACCGGATGGGGCAGGGGCTGCCCGCG
CAGTCCCTGGCCTGGGGCGCCTGGACCCAGGACGGCGGCATGACCGCCACCCTCTCCGAC
ACGGATATGCAGCGGATGGCCTCCTACGGCACCGCACCGCTGAGCCGGCAGCGCGGCCTG
GCCCTGTTCGACGCCGCCTGTGCGCGCAGCGCGGCCCACCTCGTCGCGGTCGGCCGGGTC
GGCGGGCCGGTGCGGGCCTCGGCCCCGGTCCCGTCGGTGCTGCGCGGCCTGGTCAAGGGC
GCCCGCCGCACGGCCGTGAACACGACGGGAGAGACGGCCGTGTCCCTCGCCGACCGGCTG
CGGGCCGCGCGCCCCGAGGAGCGCCCGCGCCTGATGACGGACCTGGTCCGCACCGAGGCC
GCCGCCGTCCTCGGACACGCCTCGGCGAAGAACGTCGACGCCCGGCGGGAGTTCAACGAA
CTCGGCTTCGACTCCCTGACCTCCGTCGAACTGCGCAACCGGCTCTCCGCCCTCACCGGA
CTGCGGCTGAGCGCCGCCCTCGTCTTCGACTACCCCAACCCCACCGCGCTCGCCGACCAC
TTCGTCTCGGAGCTCGTGGAAGAGGCCGCCACGGGACCGGCCCTCCTGGCAGAACTCGAA
CGGCTCGACTCCGCACTCGCCGCCGGCGCGCCCGACGCCCGAACCAGGGCCGCCGTCACC
GCCCGTCTGGCGCAGATCCTTGACGACTGGCGGGACGCCCCAGCGGAGGAGGACGGCGCA
GAGGTCGCCGAGCGGCTCGAAGCCGCCAGCACCGACGAGATCTTCGCCTTCATCGACAAC
GAGCTCGGCCGTCACAGCGATCGCTGA
[11] KS35..408
[11] AT552..857
[11] malonyl-CoA732..736
[11] DH905..1062
[11] KR1371..1551
[11] ACP1652..1722
[11] KS103..1224
[11] AT1654..2571
[11] malonyl-CoA2194..2208
[11] DH2713..3186
[11] KR4111..4653
[11] ACP4954..5166

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [542-667]  2.20000000000002e-79 G3DSA:3.40.366.10 [732-847]  2.20000000000002e-79 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
 [1371-1538]  9.69999999999989e-63 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
 [1680-1695]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1655-1720]  8.69999999999999e-11 PF00550
PF00550   PP-binding
 [1652-1722]  PS50075
PS50075   ACP_DOMAIN
 [1652-1725]  4.70000000000001e-19 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1644-1739]  1e-20 SSF47336
SSF47336   ACP_like
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [35-285]  1.79999999999997e-92 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [293-408]  1.40000000000001e-48 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [552-857]  7.20000000000003e-65 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [547-849]  4.80002938964645e-73 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [669-731]  1.29999924468179e-15 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [35-296]  2.50000000000001e-90 G3DSA:3.40.47.10 [297-463]  9.9e-66 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [27-407]  1.79999754022378e-104 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1370-1557]  9.8e-40 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [198-214]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [552-838]  2.89997807423187e-125 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1653-1725]  1.39999892049878e-32 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
 [905-1062]  5.20000089003336e-79 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [37-463]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1371-1551]  5.50001754266469e-67 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit