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CDS information : Pladi_00040


close this sectionLocation

Organism
StrainMer-11107
Entry namePladienolide
Contig
Start / Stop / Direction60,269 / 65,695 / + [in whole cluster]
60,269 / 65,695 / + [in contig]
Location60269..65695 [in whole cluster]
60269..65695 [in contig]
TypeCDS
Length5,427 bp (1,808 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase
Gene
Gene (GenBank)pldAIV
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18997414] Organization of the biosynthetic gene cluster for the polyketide antitumor macrolide, pladienolide, in Streptomyces platensis Mer-11107. (Biosci Biotechnol Biochem. , 2008)

close this sectionPKS/NRPS Module

10 malonyl-CoA
KS55..431
AT591..914
KR1162..1342
ACP1444..1514
TE1589..1804

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase]
MSQHDDASDALRTGDVPMTQFPTNEDKLRDYLKRAVTDLHHTREQLAAAEAKNREPLAIV
SMSCRFPGGVRSPEALWQLVRAGEDVISSFPTDRGWDLDGLYNPDPGNSGTTYVREGGFL
SDATEFDPAVFGISPREALGMDPQQRLMLETSWEAFERAGIGPASARGSRTGVFIGASAQ
GYSLLFQNSREEAEGLLATGDSASVISGRVSYTFGLEGPAVTLDTACSSSLVALHLAVRS
VRQGECSMALVGGVSVMCTPAIFIEFSRQRGLAADGRCKPFAAAADGTSWGEGAGVVLIE
RLEDARRNGHPVLAVIRGSAINQDGASNGLTAPHGPSQRRLIQQALADAQLSPGQIDMVE
AHGTGTSLGDPIEAQALLETYGANRPADRPLWLGSVKSNIGHTQAAAGLASVIKTVQALR
HAHLARTLHVDRPTPRVDWSSGGVELLADDQPWPETGQPRRAAVSSFGVSGTNAHVVLEQ
APASENPPLRRPGGDRVAARRVLPLVISGKTPEALRAQAGNLVSHVREHPDLRLEDLGYS
LATTRSALGHRAVVVADTPDGFLRGCEAVERGETPASVDRGVVRGRGTTAFLFTGQGAQR
VGMGRQLYAAIPAFARFLDEACSHLDRFTKQPLRDVLFAAEGSAEAALLDRTGFAQPALF
ALEVALFRTLESWGVTPDYLAGHSIGELAAAHVAGVLSLGDATRLVTARGNLMEQLPAGG
GMLALQASEAGVLPLLDGADGLVSVAAVNSPRSTVVAGDSDALAALAGQARSQGIKARHL
TVSHAFHSPLMDPVLDAYRETAEQLSYHPPRIPIISTVTGRSVTTEMSEPGYWVRHAREA
VRFTDAVATLRQHGTTAYLELGPDAVLTAMTREHLAGDGTSGKESTFAAVMRRNRPEPEV
LTSAVSQLFARGTRVDWRAVFADVDGQVVQLPTYAFQRSRYWPQASLTRPAGGASATSLF
HLRWVPVTAQDTAPADDWALLGGADALPGQGFADLASLGETIDGGSAAPRTVCVPLLPPA
DGAQDSAATHDAAHRALALAQAWLADDRFTSSRLVFLTRGAVAVTDEEYPEDSVDAFAYA
SVWGLLRSAQTENPGRFGLVDLDPDADPDAAGQRCPVPAAALDGDEPQLAMRRGVVHAPR
LTRVTAAPKDPDRAPAGFDHGGTVLITGATGGLGPLLARHLVVEHGVRHLLLTSRRGAAA
SGAQALLDELADLGAEATVVSCDLADREAVAGLLAQVPPARPLTAVVHAAGVLDDGVIPS
LSPERVDGVLRPKADGALHLHELTKDLDLAHFILFSSTAGVLGSAGQGNYAAANTFLDAL
AQHRRAAGLAAVSLAWGTWEPSGGMTGGLTRADLERMTKGGMPPLSPRDGLALFDAAIAS
GRALVVPAVLDLDLLRSRIGTNVPALLRGLIEPRPVEPSAPGEAAEALALRMASCSAAER
TGVLLDLVRADAATVLGHDGPHAIDPERGLLEAGFDSLTTLELRNRLAEATGLAVPAGYL
YEYPTPNLLAEHLAAALAESPQSGAATGADGPAEPLSVLFQQAYDLGKVTEGMTLLRSAS
ALRPTYDTPSDLSELPQPTRLARGPERATLLCFSAIVALAGSHQYSRFASSFREERDVSV
LYAPGFFAGELLPTSLETVIDTQVETVRQQAADGPVVLVGASSGGWLAHAAAARLEALGT
PPAAVVLLDTYLPDDQFLARDQDRFIGGVFDRQDRFSIREDVSLSAMGWYLHLFDGWKPT
AISVPELLVRASEPLPSPSGRPPRAADWRTSWHVAQHSVEVPGDHFTMLEEFNDATADAV
RRWLLDID
selected fasta
>polyketide synthase [polyketide synthase]
ATGAGCCAACACGACGATGCTTCTGACGCGCTGAGGACGGGCGATGTTCCGATGACACAG
TTTCCGACGAACGAGGACAAGCTCCGCGACTATCTGAAGCGGGCGGTCACCGACCTGCAC
CACACCCGTGAGCAGCTGGCCGCGGCCGAGGCCAAGAACCGGGAACCGCTGGCGATCGTG
TCGATGAGCTGTCGCTTCCCTGGCGGAGTCAGGTCGCCCGAAGCCTTGTGGCAGCTGGTG
CGTGCCGGTGAAGACGTGATCTCGTCGTTTCCCACCGACCGTGGATGGGACCTCGACGGC
CTCTACAACCCGGATCCGGGGAACAGTGGCACCACCTACGTGCGAGAGGGCGGGTTCCTG
TCCGACGCGACGGAGTTCGACCCCGCCGTGTTCGGGATCTCCCCGCGTGAGGCGCTGGGA
ATGGACCCGCAGCAGCGGCTGATGCTGGAGACCTCGTGGGAGGCCTTCGAGCGGGCCGGC
ATCGGTCCGGCATCGGCACGCGGCAGCCGGACCGGTGTGTTCATCGGCGCCTCCGCCCAG
GGCTACAGCTTGCTGTTCCAGAACTCGCGGGAGGAGGCCGAGGGCCTCCTGGCCACCGGT
GACTCGGCCAGCGTGATCTCCGGCCGGGTCTCCTACACCTTCGGCCTCGAAGGACCTGCG
GTCACACTCGACACCGCGTGCTCCTCGTCCCTGGTCGCTCTTCACCTGGCCGTGCGCTCG
GTTCGGCAGGGCGAGTGCTCCATGGCGTTGGTGGGCGGCGTCTCGGTGATGTGCACGCCG
GCGATCTTCATCGAGTTCAGCCGCCAGCGAGGTCTCGCGGCGGACGGCCGGTGCAAGCCG
TTCGCCGCGGCGGCGGACGGCACCAGCTGGGGGGAAGGCGCCGGAGTCGTCCTCATCGAG
CGGCTGGAGGACGCCCGACGCAACGGGCACCCGGTGCTGGCCGTCATCCGCGGCAGTGCC
ATCAACCAGGACGGTGCCAGCAACGGCCTGACTGCCCCGCACGGGCCGTCGCAGCGGCGG
CTGATCCAGCAGGCGCTGGCGGACGCCCAGCTGTCGCCCGGCCAGATCGACATGGTCGAG
GCACACGGCACCGGCACCTCGCTGGGGGATCCGATCGAGGCGCAGGCACTGCTGGAAACG
TACGGTGCCAACCGCCCCGCGGACCGCCCGCTCTGGCTCGGTTCCGTCAAGTCCAACATC
GGACACACCCAGGCGGCGGCCGGTCTCGCGTCCGTCATCAAGACCGTACAGGCGCTGCGA
CACGCCCACCTGGCCAGGACACTGCACGTCGACCGGCCGACCCCGCGCGTGGACTGGTCG
TCGGGTGGGGTGGAACTGCTGGCCGACGACCAGCCGTGGCCCGAGACGGGGCAGCCCCGC
CGAGCCGCCGTGTCCTCGTTCGGGGTCAGCGGCACCAACGCGCACGTCGTCCTCGAACAG
GCGCCCGCCTCGGAGAACCCGCCCCTCCGCCGTCCGGGAGGGGACCGCGTCGCGGCGCGC
CGGGTACTCCCGCTGGTGATCTCCGGCAAGACGCCGGAAGCCCTGCGGGCTCAGGCGGGG
AACCTGGTGTCCCATGTGCGCGAGCACCCGGACCTCCGGCTGGAGGACCTCGGGTACTCG
CTGGCCACCACCAGGTCGGCCCTCGGACACCGGGCCGTCGTCGTGGCGGACACCCCCGAC
GGATTCCTCCGTGGCTGCGAGGCGGTGGAGCGCGGCGAGACCCCGGCGTCGGTGGACCGG
GGCGTGGTCCGGGGGCGCGGCACGACCGCGTTCCTGTTCACGGGGCAGGGCGCCCAGCGG
GTCGGCATGGGCCGGCAGCTCTACGCGGCGATCCCCGCGTTCGCGCGGTTCCTCGACGAG
GCCTGCTCCCATCTCGACCGCTTTACGAAGCAGCCCCTGAGGGACGTGCTGTTCGCTGCC
GAGGGCAGCGCCGAGGCAGCGCTCCTGGACCGTACCGGATTCGCCCAGCCGGCCCTGTTC
GCCCTGGAGGTGGCGCTGTTCCGCACCCTGGAGTCCTGGGGTGTGACCCCGGACTACCTC
GCCGGACACTCCATCGGTGAGCTCGCTGCCGCCCATGTGGCCGGTGTGCTCTCGCTGGGA
GACGCCACCCGGCTGGTGACCGCGCGTGGCAACCTCATGGAACAGCTCCCCGCGGGGGGC
GGCATGCTCGCCCTGCAAGCTTCCGAAGCCGGGGTGCTCCCGCTCCTCGACGGCGCCGAT
GGCCTGGTGTCCGTCGCCGCCGTCAACAGCCCCCGCTCCACCGTGGTTGCCGGAGACAGC
GACGCCCTCGCCGCCCTCGCCGGCCAGGCCCGCTCTCAGGGCATCAAGGCCCGCCACCTC
ACTGTCAGCCACGCCTTCCACTCCCCGCTGATGGACCCCGTCCTCGACGCCTACCGCGAG
ACCGCCGAGCAGCTCTCCTACCACCCGCCGCGTATCCCGATCATCTCGACCGTCACCGGC
CGGTCCGTCACCACCGAGATGTCCGAACCCGGCTACTGGGTCCGGCACGCCCGCGAGGCC
GTCCGGTTCACCGATGCCGTGGCCACGCTCCGGCAGCACGGCACCACCGCCTACCTGGAA
CTCGGCCCCGACGCCGTCCTCACTGCCATGACCCGCGAACACCTGGCGGGCGACGGCACC
TCGGGCAAGGAGTCCACCTTCGCGGCGGTGATGCGCAGGAACCGGCCGGAGCCGGAGGTC
CTGACCAGCGCCGTGTCCCAGCTGTTCGCCCGGGGCACCCGCGTCGACTGGCGGGCCGTG
TTCGCGGATGTGGATGGGCAGGTCGTCCAGCTGCCGACCTACGCCTTCCAGCGCAGCCGG
TACTGGCCGCAGGCATCACTGACCCGGCCGGCCGGGGGCGCCTCCGCGACGTCGCTGTTC
CACCTGCGCTGGGTGCCGGTGACGGCCCAGGACACGGCGCCGGCGGACGACTGGGCGTTG
CTCGGCGGGGCCGACGCGCTGCCCGGCCAGGGCTTCGCCGACCTGGCGTCCCTGGGGGAG
ACGATCGACGGCGGATCGGCCGCACCCCGCACGGTGTGTGTGCCGTTGCTGCCTCCGGCC
GACGGCGCCCAGGATTCCGCCGCCACGCACGACGCCGCCCACCGGGCGCTGGCGCTGGCT
CAGGCTTGGCTCGCCGACGATCGCTTCACCTCCTCCCGGCTGGTGTTCCTCACCCGTGGT
GCGGTGGCCGTGACCGACGAGGAATACCCCGAGGACTCCGTCGACGCCTTCGCATACGCC
TCCGTGTGGGGTCTGCTGCGTTCGGCCCAGACGGAGAACCCGGGCCGGTTCGGCCTGGTG
GACCTCGACCCCGACGCCGACCCGGACGCGGCCGGGCAGCGGTGCCCGGTCCCGGCCGCC
GCCCTGGACGGCGACGAACCGCAGCTGGCGATGCGCCGAGGCGTGGTCCACGCTCCCCGG
CTCACCCGGGTCACGGCCGCGCCCAAGGACCCGGACCGGGCACCCGCCGGGTTCGACCAC
GGCGGAACCGTGCTGATCACGGGCGCCACCGGTGGACTCGGACCGCTGCTGGCCCGCCAT
CTGGTCGTCGAGCACGGCGTACGCCACCTGCTGCTGACGAGCCGTCGCGGCGCGGCGGCG
AGCGGCGCCCAGGCACTGCTGGACGAGCTCGCCGACCTGGGTGCCGAGGCCACCGTGGTC
TCCTGCGACCTGGCTGACCGGGAGGCGGTGGCCGGCCTGCTGGCCCAGGTGCCGCCCGCG
CGTCCGCTGACCGCGGTGGTGCACGCCGCGGGCGTCCTGGACGACGGCGTGATCCCGTCC
CTGAGCCCGGAACGCGTCGACGGGGTACTGCGGCCGAAGGCGGACGGGGCCCTGCACCTG
CATGAGCTGACCAAGGATCTGGACCTGGCCCACTTCATCCTGTTCTCCTCGACCGCCGGT
GTCCTCGGCAGTGCCGGCCAGGGCAACTACGCGGCCGCGAACACGTTCCTGGACGCGCTG
GCCCAGCACCGGCGGGCAGCAGGGCTGGCCGCTGTCTCGCTCGCCTGGGGAACGTGGGAA
CCGAGCGGCGGCATGACCGGCGGGCTGACGCGCGCAGACCTGGAGCGCATGACGAAGGGA
GGCATGCCACCGTTGTCCCCCCGGGACGGGCTGGCGCTCTTCGATGCCGCCATCGCTTCG
GGGCGGGCCCTGGTGGTGCCGGCCGTGCTCGATCTCGACCTGCTGCGTTCCCGGATCGGG
ACGAACGTACCGGCGCTGCTGCGCGGCCTCATCGAGCCCCGGCCCGTGGAGCCGTCTGCC
CCAGGGGAGGCAGCCGAGGCACTCGCCCTGCGGATGGCCTCCTGCTCCGCCGCGGAGCGC
ACGGGCGTACTCCTGGACCTGGTCCGCGCCGACGCGGCCACGGTGCTGGGACATGACGGT
CCGCACGCCATCGACCCGGAGCGTGGACTGCTCGAAGCGGGCTTCGACTCCCTGACGACG
CTGGAGCTGCGCAACCGGCTGGCCGAGGCCACCGGACTGGCCGTCCCGGCCGGTTACCTC
TACGAGTACCCCACCCCGAACCTGCTTGCCGAACACCTGGCGGCCGCGTTGGCCGAGTCG
CCGCAGTCCGGCGCGGCGACCGGAGCCGACGGACCGGCCGAGCCGCTGAGCGTGCTCTTC
CAGCAGGCGTATGACCTCGGCAAGGTCACCGAGGGCATGACCCTGCTCAGGAGCGCGTCC
GCGCTCCGCCCGACCTACGACACCCCTTCGGACCTCAGTGAACTGCCGCAGCCCACTCGC
CTGGCCCGTGGCCCCGAACGTGCCACGCTGCTGTGCTTCTCCGCCATCGTGGCACTCGCG
GGCTCGCACCAGTACTCGCGCTTCGCCTCGTCCTTCCGCGAGGAACGGGACGTCTCGGTC
CTCTACGCGCCGGGGTTCTTCGCCGGGGAGCTCCTGCCGACCAGCCTCGAAACGGTCATC
GACACCCAGGTGGAAACCGTGCGGCAGCAGGCCGCGGACGGTCCGGTGGTGCTCGTCGGC
GCGTCTTCCGGCGGCTGGCTCGCCCATGCCGCCGCCGCCCGGCTGGAGGCGCTGGGAACA
CCACCGGCAGCCGTGGTCCTGCTGGACACCTACCTGCCGGACGACCAGTTCCTCGCCCGT
GACCAGGACCGTTTCATCGGCGGAGTCTTCGACCGGCAGGACCGGTTCTCCATCCGGGAG
GACGTCAGCCTGTCCGCGATGGGCTGGTATCTGCACCTGTTCGACGGCTGGAAGCCCACC
GCGATCTCCGTCCCGGAACTGCTGGTCCGGGCGAGTGAGCCGCTGCCCAGCCCTTCCGGC
CGCCCGCCGAGGGCCGCCGACTGGCGGACCTCATGGCATGTGGCACAGCACAGCGTCGAG
GTGCCCGGCGATCACTTCACGATGCTGGAGGAATTCAACGACGCCACGGCCGACGCCGTC
CGACGCTGGCTTCTCGACATTGACTGA
[10] KS55..431
[10] AT591..914
[10] malonyl-CoA784..788
[10] KR1162..1342
[10] ACP1444..1514
[10] TE1589..1804
[10] KS163..1293
[10] AT1771..2742
[10] malonyl-CoA2350..2364
[10] KR3484..4026
[10] ACP4330..4542
[10] TE4765..5412

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
 [1589-1804]  3.90000000000001e-25 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
 [586-716]  5.69999999999998e-77 G3DSA:3.40.366.10 [784-903]  5.69999999999998e-77 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
 [1472-1487]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1444-1518]  5.30000000000001e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
 [1436-1531]  6.90001478563507e-20 SSF47336
SSF47336   ACP_like
 [1444-1514]  PS50075
PS50075   ACP_DOMAIN
 [1447-1513]  4.9e-11 PF00550
PF00550   PP-binding
IPR013968 Polyketide synthase, KR (Domain)
 [1162-1340]  1.09999999999999e-63 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [55-305]  6.99999999999989e-96 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [313-431]  6.80000000000006e-46 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [591-914]  3.30000000000002e-69 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
 [25-55]  4.50000034739347e-06 SSF101173
SSF101173   Polyketide_synth_docking
 [23-47]  8.80000000000003e-11 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [588-905]  6.80004680300024e-71 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [718-783]  1.70000295590054e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
 [58-317]  2.30000000000004e-91 G3DSA:3.40.47.10 [318-483]  7.00000000000005e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
 [47-483]  2.0000139277205e-104 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1161-1378]  1.09999999999999e-39 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [218-234]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [592-895]  3.19999899046355e-126 SM00827
SM00827   PKS_AT
IPR020802 Polyketide synthase, thioesterase domain (Domain)
 [1591-1804]  2.19999900980707e-102 SM00824
SM00824   PKS_TE
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1445-1517]  1.99999636034521e-26 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [57-483]  SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1162-1342]  7.80005155399507e-67 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit