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CDS information : Polk_00090


close this sectionLocation

Organism
StrainTü6028
Entry namePolyketomycin
Contig
Start / Stop / Direction10,263 / 11,291 / + [in whole cluster]
10,263 / 11,291 / + [in contig]
Location10263..11291 [in whole cluster]
10263..11291 [in contig]
TypeCDS
Length1,029 bp (342 aa)
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close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-4 ketoreductase
Product (GenBank)PokS6
Gene
Gene (GenBank)pokS6
EC number
Keyword
Note
Note (GenBank)
  • putative NDP-hexose 4-ketoreductase
Reference
ACC
PmId
[19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009)
Related Reference
ACC
O33708
NITE
Adria_00200
PmId
[9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997)
[10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999)
[9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998)

close this sectionSequence

selected fasta
>putative NDP-hexose C-4 ketoreductase [PokS6]
MTAPDVNGVVTAPEPNGVLTGPQLGGAASGPGPNAVVVGGSGFLGRHVCAALTAHGARVL
SVARRPAPGVASARIDLVGGPPAALADLLVEHGATVVVNAAGAVWDYDGRALDEANVTLV
ERLRDAVAKVPHRVRLVQLGSVFEYEMPARGRPLTEEAPTDPSTAYGRSKLRGSEIVLDA
TRAGLLDGVVLRATSCAGPGLPRSSLLGRVAARLRDAAVRGEPAVVTLAPLTAERDYVDC
RDLAAAVAAAATLPVVGRTVNIGSGTAVGVRRLVDLLVAVSGVPATVVEEGNATGRSAGI
DWLAVDGGLAARLLGWHPRHSLASTVRAMWSEVITNAEEGPD
selected fasta
>putative NDP-hexose C-4 ketoreductase [PokS6]
GTGACCGCGCCGGACGTGAACGGCGTCGTGACCGCGCCGGAGCCGAACGGCGTTCTGACC
GGGCCGCAGTTGGGCGGTGCCGCGTCCGGACCGGGGCCGAACGCCGTCGTGGTCGGCGGC
AGCGGCTTTTTGGGGCGGCACGTCTGCGCCGCGCTCACCGCGCACGGAGCCCGCGTCCTG
TCGGTCGCCCGGCGCCCCGCGCCCGGTGTGGCGTCCGCGCGGATCGACCTCGTCGGCGGA
CCTCCGGCCGCCCTCGCCGATCTCCTCGTCGAACACGGCGCGACCGTCGTCGTGAACGCC
GCCGGCGCGGTCTGGGACTACGACGGGCGGGCCCTGGACGAAGCCAACGTGACGCTCGTG
GAGCGGCTGCGCGACGCCGTCGCCAAGGTGCCCCACCGGGTCCGCCTGGTGCAGCTCGGC
TCGGTCTTCGAGTACGAGATGCCCGCGCGGGGACGGCCCCTGACGGAGGAGGCGCCGACG
GACCCGTCGACCGCATACGGCAGAAGCAAACTGAGGGGCAGTGAGATCGTCCTCGACGCC
ACCCGGGCGGGCCTGCTCGACGGGGTCGTGCTCCGCGCGACGAGCTGTGCGGGACCCGGG
CTGCCGCGCTCCAGCCTGCTGGGCCGGGTGGCCGCCCGGCTGCGCGACGCGGCGGTGCGG
GGCGAGCCGGCGGTGGTGACGCTCGCGCCCCTGACCGCCGAACGCGACTACGTGGACTGC
CGCGACCTCGCCGCCGCCGTGGCCGCCGCCGCGACCCTTCCCGTCGTCGGACGGACGGTC
AACATCGGCAGCGGCACGGCCGTCGGAGTGCGCCGGCTGGTGGACCTGCTCGTCGCCGTG
AGCGGAGTGCCCGCGACGGTCGTCGAGGAGGGCAACGCCACCGGGCGCAGCGCCGGGATC
GACTGGCTCGCCGTGGACGGCGGGCTCGCCGCGCGACTGCTCGGGTGGCACCCGCGGCAC
AGCCTGGCCTCGACGGTGCGGGCGATGTGGAGCGAGGTCATCACCAACGCCGAGGAAGGG
CCTGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
 [36-263]  5.49999999999995e-34 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
 [35-217]  7.59999999999998e-20 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit