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CDS information : Rifam_00230


close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction47,174 / 52,465 / + [in whole cluster]
47,174 / 52,465 / + [in contig]
Location47174..52465 [in whole cluster]
47174..52465 [in contig]
TypeCDS
Length5,292 bp (1,763 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)RifC
Gene
Gene (GenBank)rifC
EC number
Keyword
Note
Note (GenBank)
  • rifamycin polyketide synthase
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[10430893] Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively. (Proc Natl Acad Sci U S A. , 1999)
[23659177] Stereochemistry of reductions catalyzed by methyl-epimerizing ketoreductase domains of polyketide synthases. (J Am Chem Soc. , 2013)
[24161343] Coupled methyl group epimerization and reduction by polyketide synthase ketoreductase domains. Ketoreductase-catalyzed equilibrium isotope exchange. (J Am Chem Soc. , 2013)
[26568113] Substrate structure-activity relationships guide rational engineering of modular polyketide synthase ketoreductases. (Chem Commun (Camb). , 2016)
[27246853] Protein-Protein Interactions, Not Substrate Recognition, Dominate the Turnover of Chimeric Assembly Line Polyketide Synthases. (J Biol Chem. , 2016)

close this sectionPKS/NRPS Module

7 methylmalonyl-CoA
KS36..408
AT555..868
dh911..1070
KR1334..1509
ACP1617..1687

close this sectionSequence

selected fasta
>polyketide synthase [RifC]
MVSASYEKVVEALRKSLEEVGTLKKRNRQLADAAGEPIAIVGMACRLPGGVTGPGDLWRL
VAEGGDAVSGFPTDRCWDLDTLFDPDPDHAGTSYTDQGGFLHDAALFDPGFFGISPREAL
AMDPQQRLLLEASWEALEGVGLDPASLQGTDVGVFTGAGGSGYGGGLTGPEMQSFAGTGL
ASSVASGRVSYVFGFEGPAVTIDTACSSSLVAMHLAAQALRQGDCSMALAGGAMVMSGPD
SFVVFSRQRGLATDGRCKAFASGADGMVLAEGISVVVLERLSVARERGHRVLAVLRGSAV
NQDGASNGLTAPNGPSQQRVIRAALANAGIGPSDVDLVEAHGTGTSLGDPIEAQALLATY
GQDRETPLWLGSLKSNIGHTQAAAGVASVIKVVQALRHGVMPPTLHVDEPSSQVDWSEGA
VELLTGSRDWPRGDRPRRAGVSSFGVSGTNVHLIIEEAPEEPAAAVPTSADVVPLVVSAR
STGSLAGQADRLTEVDVPLGHLAGALVAGRAVLEERAVVVAGSAEEARAGLGALARGEAA
PGVVTGTAGKPGKVVWVFPGQGTQWVGMGRELLDASPVFAERIKECAAALDQWTDWSLLD
VLRGDGDLDSVEVLQPACFAVMVGLAAVWESAGVRPDAVVGHSQGEIAAACVSGALTLDD
AAKVVALRSQAIAARLSGRGGMASVALSEDEANARLGLWDGRIEVAAVNGPASVVIAGDA
QALDEALEVLAGDGVRVRQVAVDYASHTRHVEDIRDTLAETLAGITAQAPDVPFRSTVTG
GWVRDADVLDGGYWYRNLRNQVRFGPAVAELLEQGHGVFVEVSAHPVLVQPISELTDAVV
TGTLRRDDGGLRRLLTSMAELFVRGVRVDWATLVPPARVDLPTYAFDHQHFWLRPAAQAD
AVSLGQAAAEHPLLGAVVRLPQSDGLVFTSRLSLRTHPWLADHTIGGVVLFPGTGLVELA
VRAGDEAGCPVLDELVTEAPLVVPGQGGVNVQVTVSGPDQNGLRTVDIHSQRDDVWTRHA
TGTVSATPASSPGFDFTAWPPPDGQRVEIGDFYADLAERGYAYGPLFQGVRAVWQRGEDV
FAEVALPEDRREDAARFGLHPALLDAALQTGTIAAAASGQPGKSVMPFSWNRLALHAVGA
AGLRVRVAPGGPDALTVEAADETGAPVLTMDSLILREVALDQLDTARAGSLYRVDWTPLP
TVDSAVPAGRAEVLEAFGEEPLDLTGRVLAALQAWLSDAAEEARLVVVTRGAVPAGDGVV
SDPAGAAVWGLVRAAQAENPDRFVLLDTDGEVPLEAVLATGEPQLALRGTTFSVPRLARV
TEPAEAPLTFRPDGTVLVSGAGTLGALAARDLVTRHGVRRLVLASRRGRAAEGIDDLVAE
LTGHGAEVTVAACDVSDRDQVAALLKEHALTAVVHTAGVFDAGVTGALTRERLAKVFAPK
VDAANHLDELTRDLDLDAFIVYSSASSIFMGAGSGGYAAANAYLDGLMAARRAAGLPGLS
LAWGPWEQLTGMADTIDDLTLARMSRREGRGGVRALGSADGMELFDAALAAGQALLVPIE
LDLREVRADAAGGGTVPHLLRGLVRAGRQAARTAATEDGGLERRLAGLTVAEQEALLLDL
VRGQVAVVLGHADSSGVRADAAFKDAGFDSLTSVELRNRLRETTGLKLPATLVFDHPNPL
ALARHLRAELAVDEASPADAVLAGLAGLEAAIAAAGAPDGDRITARLRELLKAAEAAEAR
PGTSGDLDTASDEELFALVDGLD
selected fasta
>polyketide synthase [RifC]
GTGGTGAGTGCGTCGTATGAAAAGGTCGTCGAGGCGCTGCGGAAGTCGCTCGAAGAGGTC
GGCACGCTGAAGAAGCGGAACCGGCAGCTCGCCGACGCGGCCGGCGAGCCGATCGCCATC
GTCGGCATGGCCTGCCGGCTGCCCGGTGGCGTCACCGGGCCCGGTGACCTCTGGCGGCTG
GTGGCCGAGGGCGGCGACGCCGTCTCGGGGTTCCCCACCGACCGCTGCTGGGACCTGGAC
ACCCTGTTCGACCCGGATCCCGACCACGCGGGGACGTCGTACACCGACCAGGGCGGCTTC
CTCCACGACGCGGCCCTGTTCGACCCGGGCTTCTTCGGGATTTCGCCGCGCGAGGCGCTG
GCCATGGACCCGCAGCAGCGGTTGCTGCTGGAGGCGTCCTGGGAGGCGCTGGAAGGTGTC
GGCCTCGACCCGGCTTCGTTGCAGGGCACCGACGTCGGCGTGTTCACCGGCGCGGGCGGG
TCGGGCTACGGCGGCGGCCTCACCGGGCCGGAGATGCAGAGTTTCGCGGGCACCGGGCTG
GCCTCGAGCGTGGCTTCGGGCCGGGTGTCCTACGTCTTCGGGTTCGAGGGACCGGCGGTC
ACGATCGACACGGCGTGCTCGTCGTCGCTGGTGGCGATGCACCTCGCCGCGCAGGCCCTG
CGCCAAGGCGACTGCTCGATGGCACTGGCCGGCGGCGCGATGGTGATGTCGGGCCCCGAC
TCCTTCGTCGTCTTCTCCCGGCAGCGGGGGCTGGCCACCGACGGGCGGTGCAAGGCGTTC
GCGTCGGGCGCCGACGGCATGGTGCTCGCCGAGGGCATCAGCGTGGTCGTGCTGGAGCGG
CTTTCGGTCGCGCGGGAACGCGGGCACCGGGTGCTGGCCGTGCTGCGCGGCAGCGCGGTG
AACCAGGATGGCGCGTCGAACGGCCTGACCGCCCCGAACGGCCCTTCCCAGCAGCGCGTG
ATCCGCGCCGCGCTGGCCAACGCCGGAATCGGACCGTCCGATGTGGACCTCGTCGAGGCG
CACGGGACCGGGACGAGCCTGGGTGATCCCATCGAGGCGCAGGCCTTGCTGGCGACCTAC
GGCCAGGACCGGGAGACGCCGTTGTGGCTCGGCTCGCTGAAGTCGAACATCGGGCACACG
CAGGCGGCCGCGGGCGTGGCGAGCGTGATCAAGGTCGTGCAGGCGCTGCGGCACGGCGTC
ATGCCGCCGACCCTGCACGTCGACGAGCCCAGCTCGCAGGTCGACTGGTCCGAAGGCGCG
GTGGAACTGCTGACCGGGAGCCGGGACTGGCCGCGCGGGGACCGGCCGCGCCGGGCCGGG
GTGTCGTCGTTCGGCGTCAGCGGGACGAACGTGCACCTGATCATCGAGGAAGCCCCCGAG
GAGCCCGCTGCGGCCGTGCCGACGTCCGCGGACGTCGTGCCGCTGGTGGTTTCCGCACGC
AGCACGGGTTCCCTGGCCGGTCAGGCCGACCGGCTGACCGAGGTGGACGTCCCCCTCGGA
CACCTCGCCGGGGCGCTGGTGGCCGGGCGCGCGGTGCTCGAGGAACGCGCGGTCGTGGTC
GCCGGTTCGGCCGAAGAAGCCCGCGCGGGGCTGGGTGCGCTGGCTCGCGGTGAAGCCGCG
CCCGGCGTCGTGACCGGGACCGCGGGCAAGCCGGGCAAGGTCGTCTGGGTGTTCCCGGGA
CAGGGGACGCAGTGGGTGGGCATGGGCCGGGAGCTCCTCGACGCGTCCCCGGTGTTCGCC
GAGCGGATCAAGGAGTGCGCGGCGGCACTGGACCAGTGGACCGACTGGTCGCTGCTGGAC
GTCCTGCGTGGTGACGGTGACCTGGATTCTGTCGAGGTGCTGCAGCCCGCGTGCTTCGCG
GTGATGGTGGGGCTGGCCGCGGTCTGGGAGTCGGCGGGGGTCCGGCCGGACGCCGTCGTC
GGCCACTCGCAGGGCGAGATCGCCGCGGCCTGCGTGTCCGGCGCGCTCACCCTCGACGAC
GCCGCGAAGGTGGTGGCCCTGCGCAGCCAGGCGATCGCGGCGCGGCTGTCCGGCCGCGGC
GGGATGGCGTCGGTCGCGTTGAGCGAGGACGAGGCGAACGCACGGCTGGGTTTGTGGGAC
GGCCGGATCGAGGTGGCCGCGGTCAACGGCCCCGCCTCCGTGGTGATCGCGGGGGACGCC
CAAGCCCTCGACGAGGCTTTGGAGGTGCTGGCCGGGGACGGCGTCCGCGTCCGGCAGGTC
GCGGTCGACTACGCCTCCCACACCCGGCACGTCGAGGACATCCGCGACACCCTCGCCGAG
ACGCTGGCCGGGATCACCGCGCAGGCCCCGGACGTGCCGTTCCGCTCCACCGTCACCGGC
GGCTGGGTGCGGGACGCCGACGTCCTGGACGGCGGGTACTGGTACCGCAACCTGCGCAAC
CAGGTCCGGTTCGGCCCGGCCGTGGCCGAGCTGCTCGAGCAGGGCCACGGGGTGTTCGTC
GAGGTCAGCGCCCACCCCGTGCTGGTGCAGCCGATCAGCGAGCTCACCGACGCGGTCGTC
ACCGGGACGCTGCGGCGCGACGACGGCGGCCTGCGCCGCCTGCTGACGTCGATGGCCGAG
CTGTTCGTCCGCGGTGTTCGCGTCGACTGGGCCACGCTGGTGCCGCCCGCGCGCGTGGAC
CTCCCGACGTACGCCTTCGACCACCAGCACTTCTGGCTCCGGCCGGCCGCGCAGGCGGAC
GCCGTCTCGCTCGGCCAGGCCGCGGCGGAGCACCCGCTGCTCGGCGCGGTCGTCCGGCTG
CCGCAGTCGGACGGCCTGGTCTTCACCTCGCGGCTGTCGCTGCGGACGCACCCGTGGCTG
GCCGACCACACCATCGGCGGCGTGGTGCTGTTCCCCGGCACCGGGCTGGTCGAACTGGCC
GTGCGGGCCGGCGACGAGGCCGGGTGCCCGGTCCTGGACGAACTCGTGACCGAGGCGCCG
CTGGTCGTGCCCGGGCAGGGCGGAGTGAACGTCCAGGTCACGGTGAGCGGCCCGGACCAG
AACGGCTTGCGCACGGTGGACATCCACTCCCAGCGCGACGACGTGTGGACCCGGCACGCG
ACCGGAACGGTCTCGGCGACCCCGGCGAGCAGCCCCGGCTTCGACTTCACCGCGTGGCCG
CCGCCGGACGGGCAGCGCGTCGAGATCGGCGACTTCTACGCCGACCTCGCCGAGCGCGGG
TACGCGTACGGGCCCTTGTTCCAGGGCGTGCGGGCGGTGTGGCAGCGCGGCGAAGACGTG
TTCGCCGAGGTCGCGCTGCCCGAAGACCGGCGGGAGGACGCCGCCCGGTTCGGCCTGCAC
CCGGCGTTGCTGGACGCGGCCCTGCAGACCGGGACGATCGCCGCGGCCGCGTCCGGTCAG
CCGGGCAAGTCCGTGATGCCGTTCTCGTGGAACCGGCTGGCGCTGCACGCCGTCGGGGCC
GCGGGCCTCCGGGTCCGCGTGGCCCCCGGCGGACCGGACGCGCTGACCGTCGAGGCCGCC
GACGAGACCGGCGCCCCGGTCCTCACCATGGACTCGCTGATCCTGCGTGAAGTCGCCCTC
GACCAGCTGGACACTGCGCGCGCCGGCTCGCTCTACCGGGTGGACTGGACGCCACTGCCC
ACTGTGGACAGTGCGGTGCCCGCTGGTCGGGCCGAGGTGCTGGAAGCTTTCGGCGAGGAG
CCCCTGGACCTGACCGGCCGGGTGCTGGCCGCCCTGCAGGCGTGGCTTTCCGACGCGGCG
GAGGAAGCCCGCCTGGTCGTGGTGACCCGGGGTGCGGTGCCCGCCGGAGACGGTGTGGTG
AGCGATCCGGCGGGTGCCGCGGTGTGGGGCCTGGTCCGGGCCGCGCAGGCGGAGAACCCG
GACCGGTTCGTCCTGCTCGACACCGACGGCGAGGTGCCGCTGGAAGCGGTGCTGGCGACC
GGTGAGCCGCAGCTCGCGCTGCGCGGCACGACGTTCTCGGTGCCCCGGCTCGCCCGCGTC
ACCGAACCGGCGGAAGCCCCGCTGACGTTCCGTCCGGACGGGACGGTCCTGGTCTCCGGC
GCCGGGACGCTGGGTGCGCTCGCCGCCCGCGACCTCGTCACCCGGCACGGCGTCCGGCGG
CTCGTGCTGGCCAGCCGGCGCGGCCGGGCCGCCGAGGGCATCGACGACCTCGTCGCCGAG
CTGACCGGGCACGGCGCCGAAGTGACGGTCGCCGCCTGCGACGTCTCCGACCGCGACCAG
GTGGCGGCGCTGCTCAAGGAACACGCGCTGACCGCGGTGGTGCACACGGCGGGCGTGTTC
GACGCCGGTGTCACCGGCGCGCTGACCCGGGAGCGGCTGGCCAAGGTGTTCGCGCCCAAG
GTCGACGCGGCCAACCACCTCGACGAGCTGACCCGCGACCTGGACCTCGACGCGTTCATC
GTCTACTCGTCCGCCTCCTCGATCTTCATGGGCGCGGGCAGCGGCGGGTACGCGGCGGCG
AACGCCTACCTCGACGGCCTGATGGCCGCCCGGCGCGCGGCGGGCCTGCCGGGGCTGTCG
CTGGCCTGGGGCCCGTGGGAGCAGCTCACCGGCATGGCCGACACCATCGACGACCTCACC
CTGGCCCGGATGAGCCGGCGCGAAGGCCGCGGCGGCGTCCGCGCGCTCGGCTCCGCCGAC
GGCATGGAGCTGTTCGACGCCGCGCTCGCGGCCGGGCAGGCGCTGCTGGTGCCGATCGAG
CTCGACCTGCGCGAGGTGCGGGCCGACGCGGCCGGCGGCGGCACGGTGCCGCACCTGCTG
CGCGGGCTGGTCCGCGCGGGCCGGCAGGCGGCGCGGACGGCGGCCACCGAGGACGGCGGC
CTGGAACGCCGGCTGGCCGGGCTCACCGTGGCCGAACAGGAAGCGCTGCTGCTCGACCTC
GTCCGCGGTCAGGTCGCCGTCGTGCTCGGGCACGCCGACAGCTCCGGCGTCCGCGCCGAC
GCGGCGTTCAAGGACGCCGGGTTCGACTCGCTGACGTCGGTGGAGCTGCGCAACCGGCTG
CGCGAGACGACCGGCCTGAAACTGCCCGCGACGCTGGTCTTCGACCATCCGAACCCGCTG
GCACTGGCCCGGCACCTGCGGGCGGAACTCGCCGTCGACGAGGCATCCCCGGCCGATGCG
GTGCTGGCCGGGCTCGCCGGGCTGGAGGCGGCCATCGCGGCCGCCGGCGCCCCGGACGGC
GACCGGATCACCGCGCGGCTGCGGGAACTGCTCAAGGCCGCCGAGGCGGCCGAGGCCCGG
CCGGGCACCTCCGGCGATCTCGACACGGCCAGCGACGAGGAACTGTTCGCCCTCGTCGAC
GGGCTCGACTGA
[7] KS36..408
[7] AT555..868
[7] methylmalonyl-CoA744..748
[7] dh911..1070
[7] KR1334..1509
[7] ACP1617..1687
[7] KS106..1224
[7] AT1663..2604
[7] methylmalonyl-CoA2230..2244
[7] dh2731..3210
[7] KR4000..4527
[7] ACP4849..5061

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
 [743-856]  4e-73 G3DSA:3.40.366.10
G3DSA:3.40.366.10   no description
IPR006162 Phosphopantetheine attachment site (PTM)
 [1645-1660]  PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
 [1617-1690]  9.7e-20 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   no description
 [1617-1687]  19.705 PS50075
PS50075   ACP_DOMAIN
 [1609-1704]  8.9e-22 SSF47336
SSF47336   ACP-like
 [1621-1686]  3.8e-12 PF00550
PF00550   PP-binding
IPR013968 Polyketide synthase, KR (Domain)
 [1334-1508]  1.1e-49 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
 [36-283]  1e-92 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
 [292-408]  3.6e-50 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
 [555-868]  2.5e-102 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
 [553-854]  1.2e-67 SSF52151
SSF52151   FabD/lysophospholipase-like
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
 [678-743]  6.5e-18 SSF55048
SSF55048   Probable ACP-binding domain of malonyl-CoA ACP transacylase
IPR016038 Thiolase-like, subgroup (Domain)
 [38-296]  2.2e-86 G3DSA:3.40.47.10 [297-462]  6.2e-66 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
 [28-406]  4.6e-101 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
 [1547-1548]  1.4e-24 G3DSA:3.40.50.720
G3DSA:3.40.50.720   no description
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
 [197-213]  PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
 [557-848]  4.5e-120 SM00827
SM00827   Acyl transferase domain in polyketide syntha
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
 [1618-1690]  6.2e-32 SM00823
SM00823   Phosphopantetheine attachment site
IPR020807 Polyketide synthase, dehydratase domain (Domain)
 [911-1070]  3.9e-81 SM00826
SM00826   no description
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
 [38-460]  2.8e-204 SM00825
SM00825   Beta-ketoacyl synthase
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
 [1334-1509]  2.3e-43 SM00822
SM00822   no description
SignalP No significant hit
TMHMM No significant hit