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CDS information : Rubra_00390


close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction77,467 / 75,929 / - [in whole cluster]
77,467 / 75,929 / - [in contig]
Locationcomplement(75929..77467) [in whole cluster]
complement(75929..77467) [in contig]
TypeCDS
Length1,539 bp (512 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Productputative hydroxylase
Product (GenBank)putative hydroxyphenylpropionate
Gene
Gene (GenBank)rubP1
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
Related Reference
ACC
Q9AE02
NITE
Rifam_00520
PmId
[10430893] Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively. (Proc Natl Acad Sci U S A. , 1999)
[16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005)
ACC
Q84G20
NITE
Gelda2_00120
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
ACC
P77397
PmId
[9603882] Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12. (J Bacteriol. , 1998)

close this sectionSequence

selected fasta
>putative hydroxylase [putative hydroxyphenylpropionate]
MAKRPGASVGSPDMASLTPRADVLVVGCGPSGAVLASLLARRNRRVIVLERHREVFGLPR
ATSFDGETARLLAAAGIGAGLPGISVPATGYQWHGADGQVLLDIAFSDTGRYGWPDANTM
HQPALERLLGAAVAAEPTVEVRRGLTVVGVDQTDGDVTATAEDETGATHTFRAGWLVGCD
GANSVVRELAAVPMTDLGFSFEWLLCDVLLHETREFAPTNLQLCDPARPTTLVGSGPDRR
RWEFMCLPGESAADMDSAETAWRLLAPHGVTPQTAKLLRSGVYRSHARWAQQWRQGRVLL
AGDAAHLMPPFAGQGMCSGIRDASNLAWKLDLVLGGRADERLLDTYTLERRSHTKAAVLA
SVKLGRVICVTDPAAAADRDAAILANGGGRRTPPPEATALATGLLRPGAGDVIGTGRFDD
LIPAGFLLITTEEPGTVSETRRTLLSRLGATVVHIGADEQTDSGYLGYLRDRGAASVLVR
PDYHVYGTAPAGAMTALLEELLDRLAPHRPSD
selected fasta
>putative hydroxylase [putative hydroxyphenylpropionate]
ATGGCCAAGCGCCCGGGCGCATCCGTAGGGTCGCCGGACATGGCCTCCCTGACTCCGCGC
GCCGACGTTCTGGTGGTCGGCTGCGGCCCTAGCGGTGCCGTGCTGGCGAGCCTGCTGGCC
CGCCGGAACCGCCGGGTGATCGTGCTCGAGCGGCATCGCGAGGTCTTCGGGCTGCCCCGG
GCCACCAGCTTCGACGGGGAGACCGCCCGGCTGCTGGCGGCGGCGGGGATCGGCGCCGGC
CTGCCCGGCATCTCGGTGCCCGCGACCGGCTACCAGTGGCACGGCGCGGACGGGCAGGTC
CTCCTGGACATCGCCTTCAGCGACACCGGCCGGTACGGGTGGCCGGACGCCAACACGATG
CACCAGCCCGCGCTGGAGCGGCTTCTGGGCGCCGCGGTGGCGGCCGAACCGACGGTTGAG
GTGCGCCGTGGCCTGACCGTCGTAGGCGTCGACCAGACCGACGGCGACGTCACCGCGACC
GCCGAGGACGAAACCGGCGCCACCCACACCTTCCGGGCCGGCTGGCTGGTCGGCTGCGAC
GGGGCGAACAGCGTCGTCCGCGAACTGGCCGCCGTGCCGATGACCGACCTGGGGTTCTCC
TTCGAGTGGCTGCTGTGCGACGTCCTGCTGCACGAGACACGCGAGTTCGCCCCGACCAAC
CTGCAGCTGTGCGACCCGGCCCGGCCCACGACACTGGTGGGCAGCGGACCCGACCGGCGG
CGCTGGGAGTTCATGTGCCTGCCGGGGGAGTCCGCCGCCGACATGGACAGCGCCGAGACG
GCGTGGCGGCTTCTGGCACCGCACGGCGTCACGCCGCAGACGGCGAAACTGCTGCGCAGC
GGGGTCTACCGGTCCCATGCCCGGTGGGCCCAGCAGTGGCGGCAGGGCCGGGTCCTCCTC
GCCGGGGACGCGGCCCATCTGATGCCACCGTTCGCCGGCCAGGGCATGTGCTCGGGCATC
CGCGACGCCAGCAACCTGGCATGGAAACTGGACCTGGTGCTCGGCGGCCGGGCCGACGAG
CGGCTGCTCGACACCTACACGCTGGAGCGGCGCAGCCACACCAAGGCCGCCGTCCTCGCC
TCCGTCAAGCTCGGCCGGGTCATCTGCGTGACCGACCCCGCCGCCGCGGCCGACCGCGAC
GCGGCGATCCTGGCCAACGGCGGCGGCCGCCGGACGCCACCGCCCGAGGCGACAGCGCTG
GCCACCGGGCTGCTCCGACCCGGAGCCGGTGACGTGATCGGCACCGGCCGGTTCGACGAC
CTGATCCCGGCGGGCTTCCTGCTGATCACCACTGAGGAACCCGGCACCGTCAGCGAGACC
CGGCGCACCCTGCTGTCCAGGCTCGGCGCCACCGTCGTCCACATCGGAGCCGACGAACAG
ACGGACAGCGGATACCTCGGCTACCTGCGGGACCGGGGCGCGGCCTCCGTTCTGGTCCGG
CCGGACTACCACGTCTACGGCACCGCCCCCGCCGGCGCGATGACCGCGCTGCTGGAGGAA
CTGCTGGACCGGCTGGCGCCGCACCGCCCGTCAGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR002938 Monooxygenase, FAD-binding (Domain)
 [21-358]  1.2e-61 PF01494
PF01494   FAD_binding_3
IPR003042 Aromatic-ring hydroxylase-like (Domain)
 [22-44]  1.10000150671643e-34 PR00420 [172-187]  1.10000150671643e-34 PR00420 [295-310]  1.10000150671643e-34 PR00420 [310-326]  1.10000150671643e-34 PR00420 [328-346]  1.10000150671643e-34 PR00420
PR00420   RNGMNOXGNASE
SignalP
 [1-36]  0.186 Signal
Bacteria, Gram-negative   
TMHMM No significant hit