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CDS information : Salino3_00300


close this sectionLocation

Organism
StrainXM211
Entry nameSalinomycin
Contig
Start / Stop / Direction100,004 / 99,543 / - [in whole cluster]
100,004 / 99,543 / - [in contig]
Locationcomplement(99543..100004) [in whole cluster]
complement(99543..100004) [in contig]
TypeCDS
Length462 bp (153 aa)
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close this sectionAnnotation

Category3.4 other modification
Productputative epoxide hydrolase/cyclase
Product (GenBank)putative epoxide hydrolase
Gene
Gene (GenBank)slnBIII
EC number
Keyword
Note
  • Belongs to polyether epoxide hydrolase(PEH)-B group.
Note (GenBank)
  • SlnBIII
Reference
ACC
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
Related Reference
ACC
H1ZZV0
NITE
Salino_01080
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
ACC
B6ZK72
NITE
Lasal_00210
PmId
[18710235] Epoxide hydrolase Lsd19 for polyether formation in the biosynthesis of lasalocid A: direct experimental evidence on polyene-polyepoxide hypothesis in polyether biosynthesis. (J Am Chem Soc. , 2008)
[21375229] Enzymatic epoxide-opening cascades catalyzed by a pair of epoxide hydrolases in the ionophore polyether biosynthesis. (Org Lett. , 2011)
[22388816] Enzymatic catalysis of anti-Baldwin ring closure in polyether biosynthesis. (Nature. , 2012)
ACC
Q846W7
NITE
Monen_00240
PmId
[16632258] Evidence for the role of the monB genes in polyether ring formation during monensin biosynthesis. (Chem Biol. , 2006)

close this sectionSequence

selected fasta
>putative epoxide hydrolase/cyclase [putative epoxide hydrolase]
MLDEAALKELILEHTRRVNAGDVEGLLKLYSPEVRFEDPVGAGAQQGHEALRAHATGAVT
SNAVEIAGRPVAAQDGLHAAVPVTATMDYLPLGPVLTRYGVLPTPADPTGQRMQFQFLMV
IRAGADGLIEDMRAYWGTSDVALVDAAQGGAHA
selected fasta
>putative epoxide hydrolase/cyclase [putative epoxide hydrolase]
ATGCTGGATGAGGCGGCACTGAAAGAGCTGATCCTCGAACACACCAGGCGGGTCAATGCC
GGTGATGTCGAGGGCCTGCTCAAGCTGTACTCCCCCGAGGTCCGTTTCGAGGATCCGGTC
GGAGCCGGCGCCCAGCAGGGCCACGAGGCGCTGCGTGCCCATGCCACCGGCGCGGTGACC
AGCAACGCCGTGGAGATCGCGGGCAGGCCGGTGGCCGCCCAGGACGGGCTGCACGCCGCG
GTCCCGGTCACCGCGACGATGGACTACCTGCCGCTGGGGCCGGTCCTCACCCGGTACGGG
GTGCTGCCCACGCCGGCCGATCCCACCGGCCAGCGCATGCAATTCCAGTTCCTGATGGTG
ATCCGGGCGGGCGCCGACGGCCTCATCGAGGACATGCGGGCCTATTGGGGCACGAGTGAT
GTCGCTCTCGTCGACGCGGCACAAGGAGGAGCACATGCTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR009959 Polyketide cyclase SnoaL-like domain (Domain)
 [15-130]  2.2e-11 PF12680
PF12680   SnoaL_2
SignalP
 [1-20]  0.081 Signal
Eukaryota   
 [1-36]  0.052 Signal
Bacteria, Gram-negative   
TMHMM No significant hit