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CDS information : Spino_00010

Location

Organism	Saccharopolyspora spinosa
Strain	NRRL 18538
Entry name	Spinosyn
Contig	AY007564
Start / Stop / Direction	1,971 / 1,135 / - [in whole cluster] 1,971 / 1,135 / - [in contig]
Location	complement(1135..1971) [in whole cluster] complement(1135..1971) [in contig]
Type	CDS
Length	837 bp (278 aa)

Annotation

Category	5.1 general function
Product	putative LysR family transcriptional regulator
Product (GenBank)	probable lysR-type transcriptional regulator
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in spinosyn biosynthesis.
Note (GenBank)	ORF L16
Reference	ACC Q9ALP2 PmId [11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001) [11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000)
Family/Domain	FD IPR000847, IPR005119

Sequence

>putative LysR family transcriptional regulator [probable lysR-type transcriptional regulator]
METRELRYFVAVAEELHFGRAAQRLGIAQPPLSRTIAQLEQRLGVVLLQRTSRKVSLTEA
GAMLLTEGRAILGALAAAERRTQRAATSQPSLVLAAKAGASGELLAKLLDAYAAEPGAVA
VDLLLCESQPQKTLHDGRADVALLHQPFDPTAELDIEILNTEQQVAILPTSHPLASEPHV
RMADVSSLPDLPLARWPGPDGVYPDGPGVEVRNQTQLFQMIALGRTTVVMPESSRVNLLE
GLAAVPVLDAPDVTTVIAWPPHSRSRALAGLVRVATLL

>putative LysR family transcriptional regulator [probable lysR-type transcriptional regulator]
ATGGAGACGCGGGAGTTGCGGTACTTCGTTGCAGTCGCCGAGGAGTTGCACTTCGGCCGG
GCCGCCCAGCGCCTGGGCATCGCCCAGCCGCCGCTGTCGCGGACGATCGCCCAGCTCGAG
CAACGACTCGGAGTCGTGTTGCTGCAACGCACCAGCCGCAAAGTCTCGCTCACCGAAGCC
GGGGCAATGCTGCTGACCGAAGGCCGGGCGATCCTCGGCGCGCTGGCAGCAGCCGAGCGA
CGCACCCAGCGTGCCGCGACGAGCCAGCCCTCGCTAGTCCTGGCTGCCAAGGCCGGCGCC
TCCGGTGAGCTGCTGGCGAAGTTGCTCGACGCGTACGCCGCCGAGCCGGGAGCCGTGGCC
GTCGACCTGCTGCTCTGCGAATCCCAGCCCCAGAAAACGCTGCATGACGGCCGGGCCGAC
GTGGCGCTGTTGCATCAACCCTTCGACCCGACGGCCGAACTCGACATCGAAATTCTGAAC
ACCGAGCAACAAGTCGCCATTCTTCCGACCTCGCATCCGCTTGCCAGCGAGCCCCATGTA
CGGATGGCGGATGTCAGCTCACTGCCGGATCTCCCGCTTGCGCGCTGGCCCGGCCCCGAC
GGCGTCTATCCAGATGGCCCCGGCGTGGAAGTACGCAACCAGACGCAACTGTTCCAAATG
ATCGCACTCGGCCGCACTACCGTGGTCATGCCCGAATCCAGTCGCGTCAACCTGCTCGAA
GGCCTCGCCGCCGTACCGGTTCTAGACGCGCCGGACGTGACGACAGTCATCGCCTGGCCG
CCCCACAGCCGCTCCCGAGCACTCGCCGGCTTGGTCCGCGTGGCCACACTCCTCTAA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro Database:interpro:26.0	IPR000847 HTH transcriptional regulator, LysR (Domain) PF00126 HTH_1 PR00039 HTHLYSR PS50931 HTH_LYSR IPR005119 LysR, substrate-binding (Domain) PF03466 LysR_substrate IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd
SignalP	Eukaryota
TMHMM	No significant hit

first Spino_00020

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